Mutations in rpoB and fusA cause resistance to rifampicin and fusidic acid in methicillin-resistant Staphylococcus aureus strains from a tertiary hospital in Malaysia
Abstract Background The prevalence of resistance to rifampicin and fusidic acid among Malaysian strains of methicillin-resistant Staphylococcus aureus (MRSA) is increasing. This study aimed to determine the mechanisms of rifampicin and fusidic acid resistance and the genetic diversity of MRSA st...
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| Format: | Article |
| Sprog: | English English |
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Oxford University Press
2013
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| Fag: | |
| Online adgang: | http://eprints.um.edu.my/10649/1/Lim%2C_2013.pdf http://eprints.um.edu.my/10649/2/Lim%2C_2013.pdf |
| _version_ | 1825719894104080384 |
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| author | Lim, K.T. Teh, C.S.J. Mohd Yasim, M.Y. Thong, Kwai Lin |
| author_facet | Lim, K.T. Teh, C.S.J. Mohd Yasim, M.Y. Thong, Kwai Lin |
| author_sort | Lim, K.T. |
| collection | UM |
| description | Abstract
Background
The prevalence of resistance to rifampicin and fusidic acid among Malaysian strains of methicillin-resistant Staphylococcus aureus (MRSA) is increasing. This study aimed to determine the mechanisms of rifampicin and fusidic acid resistance and the genetic diversity of MRSA strains from a Malaysian tertiary hospital.
Methods
Minimum inhibitory concentrations (MIC) for 21 MRSA strains were determined by agar dilution test and Etest. The resistance genes, staphylococcal chromosome cassette mec (SCCmec) types, multilocus-sequence typing (MLST) types and spa types, were determined by PCR and DNA sequencing.
Results
MIC for rifampicin and fusidic acid resistance ranged from <1 to 8 µg/ml and from <1 to 256 µg/ml, respectively. A double mutation (484Arg/His and 517Glu/Gln) in rpoB causes high rifampicin resistance while a mutational change (461Leu/Lys) in fusA was observed in seven strains highly resistant to fusidic acid. Five of the seven were also resistant to rifampicin (MIC 8 µg/ml) and carried a mutated rpoB gene (484Arg/His). No other acquired fusidic acid resistance gene (fusB, fusC or fusD) was detected. Most (14/21) of the strains belonged to clone ST239-III-t037. Three belonged to ST22-IV-t1378 and the remaining four to ST239-III-t2029, ST239-III-t421, ST1178-IV-t1107 and ST241-III-t363, respectively.
Conclusions
The study showed that both rifampicin and fusidic acid resistance was associated with mutational change in rpoB and fusA, respectively. All rifampicin-resistant strains were from the same clone ST239-III-t037 whereas strains resistant to fusidic acid were genetically more diverse. |
| first_indexed | 2024-03-06T05:26:41Z |
| format | Article |
| id | um.eprints-10649 |
| institution | Universiti Malaya |
| language | English English |
| last_indexed | 2024-03-06T05:26:41Z |
| publishDate | 2013 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | um.eprints-106492019-03-18T08:16:34Z http://eprints.um.edu.my/10649/ Mutations in rpoB and fusA cause resistance to rifampicin and fusidic acid in methicillin-resistant Staphylococcus aureus strains from a tertiary hospital in Malaysia Lim, K.T. Teh, C.S.J. Mohd Yasim, M.Y. Thong, Kwai Lin QR Microbiology Abstract Background The prevalence of resistance to rifampicin and fusidic acid among Malaysian strains of methicillin-resistant Staphylococcus aureus (MRSA) is increasing. This study aimed to determine the mechanisms of rifampicin and fusidic acid resistance and the genetic diversity of MRSA strains from a Malaysian tertiary hospital. Methods Minimum inhibitory concentrations (MIC) for 21 MRSA strains were determined by agar dilution test and Etest. The resistance genes, staphylococcal chromosome cassette mec (SCCmec) types, multilocus-sequence typing (MLST) types and spa types, were determined by PCR and DNA sequencing. Results MIC for rifampicin and fusidic acid resistance ranged from <1 to 8 µg/ml and from <1 to 256 µg/ml, respectively. A double mutation (484Arg/His and 517Glu/Gln) in rpoB causes high rifampicin resistance while a mutational change (461Leu/Lys) in fusA was observed in seven strains highly resistant to fusidic acid. Five of the seven were also resistant to rifampicin (MIC 8 µg/ml) and carried a mutated rpoB gene (484Arg/His). No other acquired fusidic acid resistance gene (fusB, fusC or fusD) was detected. Most (14/21) of the strains belonged to clone ST239-III-t037. Three belonged to ST22-IV-t1378 and the remaining four to ST239-III-t2029, ST239-III-t421, ST1178-IV-t1107 and ST241-III-t363, respectively. Conclusions The study showed that both rifampicin and fusidic acid resistance was associated with mutational change in rpoB and fusA, respectively. All rifampicin-resistant strains were from the same clone ST239-III-t037 whereas strains resistant to fusidic acid were genetically more diverse. Oxford University Press 2013-12-13 Article PeerReviewed application/pdf en http://eprints.um.edu.my/10649/1/Lim%2C_2013.pdf application/pdf en http://eprints.um.edu.my/10649/2/Lim%2C_2013.pdf Lim, K.T. and Teh, C.S.J. and Mohd Yasim, M.Y. and Thong, Kwai Lin (2013) Mutations in rpoB and fusA cause resistance to rifampicin and fusidic acid in methicillin-resistant Staphylococcus aureus strains from a tertiary hospital in Malaysia. Transactions of the Royal Society of Tropical Medicine and Hygiene, 108 (2). pp. 112-118. ISSN 0035-9203, https://doi.org/10.1093/trstmh/trt111 |
| spellingShingle | QR Microbiology Lim, K.T. Teh, C.S.J. Mohd Yasim, M.Y. Thong, Kwai Lin Mutations in rpoB and fusA cause resistance to rifampicin and fusidic acid in methicillin-resistant Staphylococcus aureus strains from a tertiary hospital in Malaysia |
| title | Mutations in rpoB and fusA cause resistance to rifampicin and fusidic acid in methicillin-resistant Staphylococcus aureus strains from a tertiary hospital in Malaysia |
| title_full | Mutations in rpoB and fusA cause resistance to rifampicin and fusidic acid in methicillin-resistant Staphylococcus aureus strains from a tertiary hospital in Malaysia |
| title_fullStr | Mutations in rpoB and fusA cause resistance to rifampicin and fusidic acid in methicillin-resistant Staphylococcus aureus strains from a tertiary hospital in Malaysia |
| title_full_unstemmed | Mutations in rpoB and fusA cause resistance to rifampicin and fusidic acid in methicillin-resistant Staphylococcus aureus strains from a tertiary hospital in Malaysia |
| title_short | Mutations in rpoB and fusA cause resistance to rifampicin and fusidic acid in methicillin-resistant Staphylococcus aureus strains from a tertiary hospital in Malaysia |
| title_sort | mutations in rpob and fusa cause resistance to rifampicin and fusidic acid in methicillin resistant staphylococcus aureus strains from a tertiary hospital in malaysia |
| topic | QR Microbiology |
| url | http://eprints.um.edu.my/10649/1/Lim%2C_2013.pdf http://eprints.um.edu.my/10649/2/Lim%2C_2013.pdf |
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