Effect of benzylaminopurine (BAP) on in vitro proliferation and growth of pineapple (Ananas comosus L. Merr.) cv. smooth cayenne

The effect of 6-benzylaminopurine (BAP) at concentration range of 0 to 3.75 mg L-1 applied at concentration difference of a fixed increment of 0.25 mg L-1 on the in vitro shoot formation and average and total of shoot length and weight per explant of smooth cayenne pineapple were evaluated. BAP sign...

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Main Authors: Hamad, A.M., Taha, R.M.
Format: Article
Published: 2008
Subjects:
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author Hamad, A.M.
Taha, R.M.
author_facet Hamad, A.M.
Taha, R.M.
author_sort Hamad, A.M.
collection UM
description The effect of 6-benzylaminopurine (BAP) at concentration range of 0 to 3.75 mg L-1 applied at concentration difference of a fixed increment of 0.25 mg L-1 on the in vitro shoot formation and average and total of shoot length and weight per explant of smooth cayenne pineapple were evaluated. BAP significantly affected the average of shoot number, length and weight but did not affect the total length and total weight per explant. The maximum shoot formation (12 shoots/explant) occurred at 4 concentrations of BAP (1.75, 2.0, 2.25 and 3.5 mg L-1) while tallest (25 mm) and heaviest (0.60 g) shoots obtained on MS hormone free medium. Shoot formation pattern showed two sudden increases in shoot formation. The first occurred at 1.25 and second at 3.50 mg L-1 and two sharp declines at 2.5 and 3.75 mg L-1. Identifying of the concentrations with contrasting effect where an increase and decline in the shoot formation pattern occur provide an essential base from which an informative successful histological and biochemical studies could be designed to elucidate shoot formation mechanism. © 2008 Asian Network for Scientific Information.
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spelling um.eprints-107202014-07-21T01:17:08Z http://eprints.um.edu.my/10720/ Effect of benzylaminopurine (BAP) on in vitro proliferation and growth of pineapple (Ananas comosus L. Merr.) cv. smooth cayenne Hamad, A.M. Taha, R.M. QH301 Biology The effect of 6-benzylaminopurine (BAP) at concentration range of 0 to 3.75 mg L-1 applied at concentration difference of a fixed increment of 0.25 mg L-1 on the in vitro shoot formation and average and total of shoot length and weight per explant of smooth cayenne pineapple were evaluated. BAP significantly affected the average of shoot number, length and weight but did not affect the total length and total weight per explant. The maximum shoot formation (12 shoots/explant) occurred at 4 concentrations of BAP (1.75, 2.0, 2.25 and 3.5 mg L-1) while tallest (25 mm) and heaviest (0.60 g) shoots obtained on MS hormone free medium. Shoot formation pattern showed two sudden increases in shoot formation. The first occurred at 1.25 and second at 3.50 mg L-1 and two sharp declines at 2.5 and 3.75 mg L-1. Identifying of the concentrations with contrasting effect where an increase and decline in the shoot formation pattern occur provide an essential base from which an informative successful histological and biochemical studies could be designed to elucidate shoot formation mechanism. © 2008 Asian Network for Scientific Information. 2008 Article PeerReviewed Hamad, A.M. and Taha, R.M. (2008) Effect of benzylaminopurine (BAP) on in vitro proliferation and growth of pineapple (Ananas comosus L. Merr.) cv. smooth cayenne. Journal of Applied Sciences, 8 (22). pp. 4180-4185. ISSN 1812-5654,
spellingShingle QH301 Biology
Hamad, A.M.
Taha, R.M.
Effect of benzylaminopurine (BAP) on in vitro proliferation and growth of pineapple (Ananas comosus L. Merr.) cv. smooth cayenne
title Effect of benzylaminopurine (BAP) on in vitro proliferation and growth of pineapple (Ananas comosus L. Merr.) cv. smooth cayenne
title_full Effect of benzylaminopurine (BAP) on in vitro proliferation and growth of pineapple (Ananas comosus L. Merr.) cv. smooth cayenne
title_fullStr Effect of benzylaminopurine (BAP) on in vitro proliferation and growth of pineapple (Ananas comosus L. Merr.) cv. smooth cayenne
title_full_unstemmed Effect of benzylaminopurine (BAP) on in vitro proliferation and growth of pineapple (Ananas comosus L. Merr.) cv. smooth cayenne
title_short Effect of benzylaminopurine (BAP) on in vitro proliferation and growth of pineapple (Ananas comosus L. Merr.) cv. smooth cayenne
title_sort effect of benzylaminopurine bap on in vitro proliferation and growth of pineapple ananas comosus l merr cv smooth cayenne
topic QH301 Biology
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