Exochelin production in Mycobacterium neoaurum.
Mycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37 masculineC within 3 - 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most my...
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| Format: | Article |
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MDPI
2009
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| _version_ | 1796944690955681792 |
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| author | Chan, K. |
| author_facet | Chan, K. |
| author_sort | Chan, K. |
| collection | UM |
| description | Mycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37 masculineC within 3 - 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most mycobacteria produce siderophores. One example is exochelin, which is extracellular and water-soluble. In this report, the production of exochelin in M. neoaurum was induced in iron-deficiency, but repressed under ironsufficiency growth conditions. It is however not induced under zinc-deficiency growth conditions. The growth of this mycobacterium was correlated with exochelin secretion under iron-deficiency culture conditions. When M. neoaurum was grown in defined medium containing 0.04 microg Fe(III)/mL (final concentration), the production of exochelin reached a maximum and the corresponding cell growth was comparable to that under iron-sufficiency conditions. In this study, exochelin was purified from spent supernatant of M. neoaurum by semi-preparative chromatography. When saturated ferric chloride solution was added into the purified exochelin, a ferri-exochelin complex was formed. It is proposed that iron uptake in M. neoaurum is exochelin-mediated. |
| first_indexed | 2024-03-06T05:05:28Z |
| format | Article |
| id | um.eprints-1081 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:05:28Z |
| publishDate | 2009 |
| publisher | MDPI |
| record_format | dspace |
| spelling | um.eprints-10812017-07-08T05:42:28Z http://eprints.um.edu.my/1081/ Exochelin production in Mycobacterium neoaurum. Chan, K. RB Pathology Mycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37 masculineC within 3 - 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most mycobacteria produce siderophores. One example is exochelin, which is extracellular and water-soluble. In this report, the production of exochelin in M. neoaurum was induced in iron-deficiency, but repressed under ironsufficiency growth conditions. It is however not induced under zinc-deficiency growth conditions. The growth of this mycobacterium was correlated with exochelin secretion under iron-deficiency culture conditions. When M. neoaurum was grown in defined medium containing 0.04 microg Fe(III)/mL (final concentration), the production of exochelin reached a maximum and the corresponding cell growth was comparable to that under iron-sufficiency conditions. In this study, exochelin was purified from spent supernatant of M. neoaurum by semi-preparative chromatography. When saturated ferric chloride solution was added into the purified exochelin, a ferri-exochelin complex was formed. It is proposed that iron uptake in M. neoaurum is exochelin-mediated. MDPI 2009-01 Article PeerReviewed Chan, K. (2009) Exochelin production in Mycobacterium neoaurum. International Journal of Molecular Sciences, 10 (1). pp. 345-53. ISSN 1661-6596, DOI 19333449. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2662466/ 19333449 |
| spellingShingle | RB Pathology Chan, K. Exochelin production in Mycobacterium neoaurum. |
| title | Exochelin production in Mycobacterium neoaurum. |
| title_full | Exochelin production in Mycobacterium neoaurum. |
| title_fullStr | Exochelin production in Mycobacterium neoaurum. |
| title_full_unstemmed | Exochelin production in Mycobacterium neoaurum. |
| title_short | Exochelin production in Mycobacterium neoaurum. |
| title_sort | exochelin production in mycobacterium neoaurum |
| topic | RB Pathology |
| work_keys_str_mv | AT chank exochelinproductioninmycobacteriumneoaurum |