Helminth Colonization Is Associated with Increased Diversity of the Gut Microbiota
Soil-transmitted helminths colonize more than 1.5 billion people worldwide, yet little is known about how they interact with bacterial communities in the gut microbiota. Differences in the gut microbiota between individuals living in developed and developing countries may be partly due to the presen...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Public Library of Science
2014
|
| Subjects: |
| _version_ | 1796946346722197504 |
|---|---|
| author | Lee, S.C. Tang, M.S. Lim, Y.A.L. Choy, S.H. Kurtz, Z.D. Cox, L.M. Gundra, U.M. Cho, I. Bonneau, R. Blaser, M.J. Chua, K.H. Loke, P. |
| author_facet | Lee, S.C. Tang, M.S. Lim, Y.A.L. Choy, S.H. Kurtz, Z.D. Cox, L.M. Gundra, U.M. Cho, I. Bonneau, R. Blaser, M.J. Chua, K.H. Loke, P. |
| author_sort | Lee, S.C. |
| collection | UM |
| description | Soil-transmitted helminths colonize more than 1.5 billion people worldwide, yet little is known about how they interact with bacterial communities in the gut microbiota. Differences in the gut microbiota between individuals living in developed and developing countries may be partly due to the presence of helminths, since they predominantly infect individuals from developing countries, such as the indigenous communities in Malaysia we examine in this work. We compared the composition and diversity of bacterial communities from the fecal microbiota of 51 people from two villages in Malaysia, of which 36 (70.6%) were infected by helminths. The 16S rRNA V4 region was sequenced at an average of nineteen thousand sequences per samples. Helminth-colonized individuals had greater species richness and number of observed OTUs with enrichment of Paraprevotellaceae, especially with Trichuris infection. We developed a new approach of combining centered log-ratio (clr) transformation for OTU relative abundances with sparse Partial Least Squares Discriminant Analysis (sPLS-DA) to enable more robust predictions of OTU interrelationships. These results suggest that helminths may have an impact on the diversity, bacterial community structure and function of the gut microbiota. |
| first_indexed | 2024-03-06T05:30:19Z |
| format | Article |
| id | um.eprints-12085 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:30:19Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | um.eprints-120852015-01-15T03:41:36Z http://eprints.um.edu.my/12085/ Helminth Colonization Is Associated with Increased Diversity of the Gut Microbiota Lee, S.C. Tang, M.S. Lim, Y.A.L. Choy, S.H. Kurtz, Z.D. Cox, L.M. Gundra, U.M. Cho, I. Bonneau, R. Blaser, M.J. Chua, K.H. Loke, P. R Medicine Soil-transmitted helminths colonize more than 1.5 billion people worldwide, yet little is known about how they interact with bacterial communities in the gut microbiota. Differences in the gut microbiota between individuals living in developed and developing countries may be partly due to the presence of helminths, since they predominantly infect individuals from developing countries, such as the indigenous communities in Malaysia we examine in this work. We compared the composition and diversity of bacterial communities from the fecal microbiota of 51 people from two villages in Malaysia, of which 36 (70.6%) were infected by helminths. The 16S rRNA V4 region was sequenced at an average of nineteen thousand sequences per samples. Helminth-colonized individuals had greater species richness and number of observed OTUs with enrichment of Paraprevotellaceae, especially with Trichuris infection. We developed a new approach of combining centered log-ratio (clr) transformation for OTU relative abundances with sparse Partial Least Squares Discriminant Analysis (sPLS-DA) to enable more robust predictions of OTU interrelationships. These results suggest that helminths may have an impact on the diversity, bacterial community structure and function of the gut microbiota. Public Library of Science 2014-05 Article PeerReviewed Lee, S.C. and Tang, M.S. and Lim, Y.A.L. and Choy, S.H. and Kurtz, Z.D. and Cox, L.M. and Gundra, U.M. and Cho, I. and Bonneau, R. and Blaser, M.J. and Chua, K.H. and Loke, P. (2014) Helminth Colonization Is Associated with Increased Diversity of the Gut Microbiota. PLoS Neglected Tropical Diseases, 8 (5). e2880. ISSN 1935-2727, DOI https://doi.org/10.1371/journal.pntd.0002880 <https://doi.org/10.1371/journal.pntd.0002880>. http://www.plosntds.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pntd.0002880&representation=PDF http://dx.doi.org/10.1371/journal.pntd.0002880 |
| spellingShingle | R Medicine Lee, S.C. Tang, M.S. Lim, Y.A.L. Choy, S.H. Kurtz, Z.D. Cox, L.M. Gundra, U.M. Cho, I. Bonneau, R. Blaser, M.J. Chua, K.H. Loke, P. Helminth Colonization Is Associated with Increased Diversity of the Gut Microbiota |
| title | Helminth Colonization Is Associated with Increased Diversity of the Gut Microbiota |
| title_full | Helminth Colonization Is Associated with Increased Diversity of the Gut Microbiota |
| title_fullStr | Helminth Colonization Is Associated with Increased Diversity of the Gut Microbiota |
| title_full_unstemmed | Helminth Colonization Is Associated with Increased Diversity of the Gut Microbiota |
| title_short | Helminth Colonization Is Associated with Increased Diversity of the Gut Microbiota |
| title_sort | helminth colonization is associated with increased diversity of the gut microbiota |
| topic | R Medicine |
| work_keys_str_mv | AT leesc helminthcolonizationisassociatedwithincreaseddiversityofthegutmicrobiota AT tangms helminthcolonizationisassociatedwithincreaseddiversityofthegutmicrobiota AT limyal helminthcolonizationisassociatedwithincreaseddiversityofthegutmicrobiota AT choysh helminthcolonizationisassociatedwithincreaseddiversityofthegutmicrobiota AT kurtzzd helminthcolonizationisassociatedwithincreaseddiversityofthegutmicrobiota AT coxlm helminthcolonizationisassociatedwithincreaseddiversityofthegutmicrobiota AT gundraum helminthcolonizationisassociatedwithincreaseddiversityofthegutmicrobiota AT choi helminthcolonizationisassociatedwithincreaseddiversityofthegutmicrobiota AT bonneaur helminthcolonizationisassociatedwithincreaseddiversityofthegutmicrobiota AT blasermj helminthcolonizationisassociatedwithincreaseddiversityofthegutmicrobiota AT chuakh helminthcolonizationisassociatedwithincreaseddiversityofthegutmicrobiota AT lokep helminthcolonizationisassociatedwithincreaseddiversityofthegutmicrobiota |