Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense

Basal stem rot is a common disease that affects oil palm, causing loss of yield and finally killing the trees. The disease, caused by fungus Ganoderma boninense, devastates thousands of hectares of oil palm plantings in Southeast Asia every year. In the present study, root proteins of healthy oil pa...

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Main Authors: Al-Obaidi, J.R., Mohd-Yusuf, Yusmin, Razali, N., Jayapalan, J.J., Chin-Chong, T., Md-Noh, N., Junit, Sarni Mat, Othman, Rofina Yasmin, Hashim, Onn Haji
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Published: MDPI 2014
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author Al-Obaidi, J.R.
Mohd-Yusuf, Yusmin
Razali, N.
Jayapalan, J.J.
Chin-Chong, T.
Md-Noh, N.
Junit, Sarni Mat
Othman, Rofina Yasmin
Hashim, Onn Haji
author_facet Al-Obaidi, J.R.
Mohd-Yusuf, Yusmin
Razali, N.
Jayapalan, J.J.
Chin-Chong, T.
Md-Noh, N.
Junit, Sarni Mat
Othman, Rofina Yasmin
Hashim, Onn Haji
author_sort Al-Obaidi, J.R.
collection UM
description Basal stem rot is a common disease that affects oil palm, causing loss of yield and finally killing the trees. The disease, caused by fungus Ganoderma boninense, devastates thousands of hectares of oil palm plantings in Southeast Asia every year. In the present study, root proteins of healthy oil palm seedlings, and those infected with G. boninense, were analyzed by 2-dimensional gel electrophoresis (2-DE). When the 2-DE profiles were analyzed for proteins, which exhibit consistent significant change of abundance upon infection with G. boninense, 21 passed our screening criteria. Subsequent analyses by mass spectrometry and database search identified caffeoyl-CoA O-methyltransferase, caffeic acid O-methyltransferase, enolase, fructokinase, cysteine synthase, malate dehydrogenase, and ATP synthase as among proteins of which abundances were markedly altered.
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spelling um.eprints-123762019-10-26T04:43:20Z http://eprints.um.edu.my/12376/ Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense Al-Obaidi, J.R. Mohd-Yusuf, Yusmin Razali, N. Jayapalan, J.J. Chin-Chong, T. Md-Noh, N. Junit, Sarni Mat Othman, Rofina Yasmin Hashim, Onn Haji Q Science (General) Basal stem rot is a common disease that affects oil palm, causing loss of yield and finally killing the trees. The disease, caused by fungus Ganoderma boninense, devastates thousands of hectares of oil palm plantings in Southeast Asia every year. In the present study, root proteins of healthy oil palm seedlings, and those infected with G. boninense, were analyzed by 2-dimensional gel electrophoresis (2-DE). When the 2-DE profiles were analyzed for proteins, which exhibit consistent significant change of abundance upon infection with G. boninense, 21 passed our screening criteria. Subsequent analyses by mass spectrometry and database search identified caffeoyl-CoA O-methyltransferase, caffeic acid O-methyltransferase, enolase, fructokinase, cysteine synthase, malate dehydrogenase, and ATP synthase as among proteins of which abundances were markedly altered. MDPI 2014 Article PeerReviewed Al-Obaidi, J.R. and Mohd-Yusuf, Yusmin and Razali, N. and Jayapalan, J.J. and Chin-Chong, T. and Md-Noh, N. and Junit, Sarni Mat and Othman, Rofina Yasmin and Hashim, Onn Haji (2014) Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense. International Journal of Molecular Sciences, 15 (3). pp. 51755-5192. ISSN 1661-6596, DOI https://doi.org/10.3390/ijms15035175 <https://doi.org/10.3390/ijms15035175>. http://www.mdpi.com/1422-0067/15/3/5175 http://doi.org/10.3390/ijms15035175
spellingShingle Q Science (General)
Al-Obaidi, J.R.
Mohd-Yusuf, Yusmin
Razali, N.
Jayapalan, J.J.
Chin-Chong, T.
Md-Noh, N.
Junit, Sarni Mat
Othman, Rofina Yasmin
Hashim, Onn Haji
Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense
title Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense
title_full Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense
title_fullStr Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense
title_full_unstemmed Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense
title_short Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense
title_sort identification of proteins of altered abundance in oil palm infected with ganoderma boninense
topic Q Science (General)
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