2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans
Candida albicans is capable of undergoing yeast-hypha transition to attain pathogenicity in humans. In this study, we investigated the differential expression of CaSIR2 via quantitative real-time PCR (qPCR), during yeast-hypha transition with and without the presence of 2-dodecanol. SIR2 transcript...
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| Format: | Article |
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John Wiley & Sons
2009
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| _version_ | 1796946497459191808 |
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| author | Lim, C.S.Y. Wong, W.F. Rosli, R. Ng, K.P. Seow, H.F. Chong, P.P. |
| author_facet | Lim, C.S.Y. Wong, W.F. Rosli, R. Ng, K.P. Seow, H.F. Chong, P.P. |
| author_sort | Lim, C.S.Y. |
| collection | UM |
| description | Candida albicans is capable of undergoing yeast-hypha transition to attain pathogenicity in humans. In this study, we investigated the differential expression of CaSIR2 via quantitative real-time PCR (qPCR), during yeast-hypha transition with and without the presence of 2-dodecanol. SIR2 transcript levels were found to be significantly enhanced after hyphal induction as compared to the yeast form. This study found that 2-dodecanol is able to inhibit hyphal development and block SIR2 up-regulation, even in hyphal-inducing growth conditions. We suggest that SIR2 may be involved in Candida albicans quorum-sensing and serum-induced yeast-hyphae transition via the Ras1-cAMP-Efg1 signalling cascade. |
| first_indexed | 2024-03-06T05:32:41Z |
| format | Article |
| id | um.eprints-13022 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:32:41Z |
| publishDate | 2009 |
| publisher | John Wiley & Sons |
| record_format | dspace |
| spelling | um.eprints-130222015-03-13T01:14:29Z http://eprints.um.edu.my/13022/ 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans Lim, C.S.Y. Wong, W.F. Rosli, R. Ng, K.P. Seow, H.F. Chong, P.P. R Medicine Candida albicans is capable of undergoing yeast-hypha transition to attain pathogenicity in humans. In this study, we investigated the differential expression of CaSIR2 via quantitative real-time PCR (qPCR), during yeast-hypha transition with and without the presence of 2-dodecanol. SIR2 transcript levels were found to be significantly enhanced after hyphal induction as compared to the yeast form. This study found that 2-dodecanol is able to inhibit hyphal development and block SIR2 up-regulation, even in hyphal-inducing growth conditions. We suggest that SIR2 may be involved in Candida albicans quorum-sensing and serum-induced yeast-hyphae transition via the Ras1-cAMP-Efg1 signalling cascade. John Wiley & Sons 2009-11 Article PeerReviewed Lim, C.S.Y. and Wong, W.F. and Rosli, R. and Ng, K.P. and Seow, H.F. and Chong, P.P. (2009) 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans. Journal of Basic Microbiology, 49 (6). pp. 579-583. ISSN 0233-111X, DOI https://doi.org/10.1002/jobm.200900035 <https://doi.org/10.1002/jobm.200900035>. http://onlinelibrary.wiley.com/doi/10.1002/jobm.200900035/abstract;jsessionid=FF932426565968444C3570783479C42F.f04t04 http://dx.doi.org/10.1002/jobm.200900035 |
| spellingShingle | R Medicine Lim, C.S.Y. Wong, W.F. Rosli, R. Ng, K.P. Seow, H.F. Chong, P.P. 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans |
| title | 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans |
| title_full | 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans |
| title_fullStr | 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans |
| title_full_unstemmed | 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans |
| title_short | 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans |
| title_sort | 2 dodecanol decyl methyl carbinol inhibits hyphal formation and sir2 expression in c albicans |
| topic | R Medicine |
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