Assessment of Polymerase Chain Reaction in the Detection of Pseudomonas aeruginosa in Contact Lens-Induced Severe Infectious Keratitis
Purpose: The aim of this study is to evaluate the role of real-time polymerase chain reaction (PCR) and conventional bacterial culture methods in the detection of Pseudomonas aeruginosa in contact lens-induced severe, partially treated corneal ulcers referred to a tertiary center. Methods: The study...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Published: |
Lippincott, Williams & Wilkins
2010
|
| Subjects: |
| _version_ | 1796946514166153216 |
|---|---|
| author | Subrayan, Visvaraja Peyman, Mohammadreza Yap, Soon Lek Ali, Nadir Ali Mohamed Devi, Shamala |
| author_facet | Subrayan, Visvaraja Peyman, Mohammadreza Yap, Soon Lek Ali, Nadir Ali Mohamed Devi, Shamala |
| author_sort | Subrayan, Visvaraja |
| collection | UM |
| description | Purpose: The aim of this study is to evaluate the role of real-time polymerase chain reaction (PCR) and conventional bacterial culture methods in the detection of Pseudomonas aeruginosa in contact lens-induced severe, partially treated corneal ulcers referred to a tertiary center. Methods: The study duration was 6 months. All patients with contact lens-related corneal ulcer, requiring admission during the study period were recruited. Samples from corneal scrapings were simultaneously sent at the time of admission for PCR and culture testing. An in-house real-time PCR was developed to detect the P. aeruginosa lasA gene. The results of PCR and culture were compared using McNemar's chi(2) test. Results: Ten patients were recruited. The mean age was 33 years (20-45 years). All the patients had contact lens-related keratitis (> 4 mm) of which eight (80%) were found positive for P. aeruginosa by PCR or culture. There was no significant difference between PCR and culture in detecting P. aeruginosa (P < 0.05). Conclusions: PCR is, at least, as good as conventional cultures in detecting P. aeruginosa. It is a rapid assay as compared with culture, and early detection enables prompt treatment thus reducing the destructive effect of the organism on the cornea. |
| first_indexed | 2024-03-06T05:32:56Z |
| format | Article |
| id | um.eprints-13107 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:32:56Z |
| publishDate | 2010 |
| publisher | Lippincott, Williams & Wilkins |
| record_format | dspace |
| spelling | um.eprints-131072019-02-13T03:32:12Z http://eprints.um.edu.my/13107/ Assessment of Polymerase Chain Reaction in the Detection of Pseudomonas aeruginosa in Contact Lens-Induced Severe Infectious Keratitis Subrayan, Visvaraja Peyman, Mohammadreza Yap, Soon Lek Ali, Nadir Ali Mohamed Devi, Shamala R Medicine Purpose: The aim of this study is to evaluate the role of real-time polymerase chain reaction (PCR) and conventional bacterial culture methods in the detection of Pseudomonas aeruginosa in contact lens-induced severe, partially treated corneal ulcers referred to a tertiary center. Methods: The study duration was 6 months. All patients with contact lens-related corneal ulcer, requiring admission during the study period were recruited. Samples from corneal scrapings were simultaneously sent at the time of admission for PCR and culture testing. An in-house real-time PCR was developed to detect the P. aeruginosa lasA gene. The results of PCR and culture were compared using McNemar's chi(2) test. Results: Ten patients were recruited. The mean age was 33 years (20-45 years). All the patients had contact lens-related keratitis (> 4 mm) of which eight (80%) were found positive for P. aeruginosa by PCR or culture. There was no significant difference between PCR and culture in detecting P. aeruginosa (P < 0.05). Conclusions: PCR is, at least, as good as conventional cultures in detecting P. aeruginosa. It is a rapid assay as compared with culture, and early detection enables prompt treatment thus reducing the destructive effect of the organism on the cornea. Lippincott, Williams & Wilkins 2010 Article PeerReviewed Subrayan, Visvaraja and Peyman, Mohammadreza and Yap, Soon Lek and Ali, Nadir Ali Mohamed and Devi, Shamala (2010) Assessment of Polymerase Chain Reaction in the Detection of Pseudomonas aeruginosa in Contact Lens-Induced Severe Infectious Keratitis. Eye and Contact Lens, 36 (4). pp. 201-203. ISSN 1542-2321, DOI https://doi.org/10.1097/ICL.0b013e3181e3efa3 <https://doi.org/10.1097/ICL.0b013e3181e3efa3>. https://insights.ovid.com/pubmed?pmid=20531205 doi:10.1097/ICL.0b013e3181e3efa3 |
| spellingShingle | R Medicine Subrayan, Visvaraja Peyman, Mohammadreza Yap, Soon Lek Ali, Nadir Ali Mohamed Devi, Shamala Assessment of Polymerase Chain Reaction in the Detection of Pseudomonas aeruginosa in Contact Lens-Induced Severe Infectious Keratitis |
| title | Assessment of Polymerase Chain Reaction in the Detection of Pseudomonas aeruginosa in Contact Lens-Induced Severe Infectious Keratitis |
| title_full | Assessment of Polymerase Chain Reaction in the Detection of Pseudomonas aeruginosa in Contact Lens-Induced Severe Infectious Keratitis |
| title_fullStr | Assessment of Polymerase Chain Reaction in the Detection of Pseudomonas aeruginosa in Contact Lens-Induced Severe Infectious Keratitis |
| title_full_unstemmed | Assessment of Polymerase Chain Reaction in the Detection of Pseudomonas aeruginosa in Contact Lens-Induced Severe Infectious Keratitis |
| title_short | Assessment of Polymerase Chain Reaction in the Detection of Pseudomonas aeruginosa in Contact Lens-Induced Severe Infectious Keratitis |
| title_sort | assessment of polymerase chain reaction in the detection of pseudomonas aeruginosa in contact lens induced severe infectious keratitis |
| topic | R Medicine |
| work_keys_str_mv | AT subrayanvisvaraja assessmentofpolymerasechainreactioninthedetectionofpseudomonasaeruginosaincontactlensinducedsevereinfectiouskeratitis AT peymanmohammadreza assessmentofpolymerasechainreactioninthedetectionofpseudomonasaeruginosaincontactlensinducedsevereinfectiouskeratitis AT yapsoonlek assessmentofpolymerasechainreactioninthedetectionofpseudomonasaeruginosaincontactlensinducedsevereinfectiouskeratitis AT alinadiralimohamed assessmentofpolymerasechainreactioninthedetectionofpseudomonasaeruginosaincontactlensinducedsevereinfectiouskeratitis AT devishamala assessmentofpolymerasechainreactioninthedetectionofpseudomonasaeruginosaincontactlensinducedsevereinfectiouskeratitis |