Taxonomic Status of Three Types of Fejervarya cancrivora from Indonesia and Other Asian Countries Based on Morphological Observations and Crossing Experiments
Although the crab-eating frog Fejervarya cancrivora is one of the most widely distributed species in Asian region, taxonomic relationships among different populations remain unclarified. In this study, we attempted to elucidate the taxonomic status of F. cancrivora from Indonesian and other Asian po...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Zoological Society of Japan
2011
|
| Subjects: |
| _version_ | 1825720333755219968 |
|---|---|
| author | Kurniawan, N. Djong, T.H. Islam, M.M. Nishizawa, T. Belabut, D.M. Yong, Hoi Sen Yasir, I. Sumida, M. |
| author_facet | Kurniawan, N. Djong, T.H. Islam, M.M. Nishizawa, T. Belabut, D.M. Yong, Hoi Sen Yasir, I. Sumida, M. |
| author_sort | Kurniawan, N. |
| collection | UM |
| description | Although the crab-eating frog Fejervarya cancrivora is one of the most widely distributed species in Asian region, taxonomic relationships among different populations remain unclarified. In this study, we attempted to elucidate the taxonomic status of F. cancrivora from Indonesian and other Asian populations. Five populations of F. cancrivora from Selangor (Malaysia), Cianjur (Java, Indonesia), Trat (Thailand), Khulna (Bangladesh), and Makassar (Sulawesi, Indonesia) were morphologically observed and subjected to crossing experiments. Principal component and clustering analyses revealed that these five populations could be organized into three groups corresponding to three observed morphological types: a Selangor and Cianjur group (large-type), a Trat and Khulna group (mangrove-type), and a Makassar group (Sulawesi-type). The limited crossing experiments revealed that hybrids between Selangor females and Cianjur and Trat males developed normally, whereas hybrids between Selangor females and Khulna males showed incomplete gametic isolation. Histological observations of the testes of mature males revealed the presence of pycnotic nuclei in the hybrids between Selangor females and Khulna males in addition to normal bundles of spermatozoa. In contrast, no pycnotic nuclei were observed in the Selangor controls. Although meiotic metaphases in the controls were normal, those in hybrids showed several abnormalities, such as the appearance of univalents and an increase in rod-shaped bivalents. Based on our findings from the morphological observations and crossing experiments, we conclude that each of three identified types represents a distinct species. We propose that the large-type is F. cancrivora, the mangrove-type is F. moodiei, and the Sulawesi-type represents an undescribed species. |
| first_indexed | 2024-03-06T05:33:32Z |
| format | Article |
| id | um.eprints-13336 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:33:32Z |
| publishDate | 2011 |
| publisher | Zoological Society of Japan |
| record_format | dspace |
| spelling | um.eprints-133362019-12-05T04:27:43Z http://eprints.um.edu.my/13336/ Taxonomic Status of Three Types of Fejervarya cancrivora from Indonesia and Other Asian Countries Based on Morphological Observations and Crossing Experiments Kurniawan, N. Djong, T.H. Islam, M.M. Nishizawa, T. Belabut, D.M. Yong, Hoi Sen Yasir, I. Sumida, M. Q Science (General) QH Natural history Although the crab-eating frog Fejervarya cancrivora is one of the most widely distributed species in Asian region, taxonomic relationships among different populations remain unclarified. In this study, we attempted to elucidate the taxonomic status of F. cancrivora from Indonesian and other Asian populations. Five populations of F. cancrivora from Selangor (Malaysia), Cianjur (Java, Indonesia), Trat (Thailand), Khulna (Bangladesh), and Makassar (Sulawesi, Indonesia) were morphologically observed and subjected to crossing experiments. Principal component and clustering analyses revealed that these five populations could be organized into three groups corresponding to three observed morphological types: a Selangor and Cianjur group (large-type), a Trat and Khulna group (mangrove-type), and a Makassar group (Sulawesi-type). The limited crossing experiments revealed that hybrids between Selangor females and Cianjur and Trat males developed normally, whereas hybrids between Selangor females and Khulna males showed incomplete gametic isolation. Histological observations of the testes of mature males revealed the presence of pycnotic nuclei in the hybrids between Selangor females and Khulna males in addition to normal bundles of spermatozoa. In contrast, no pycnotic nuclei were observed in the Selangor controls. Although meiotic metaphases in the controls were normal, those in hybrids showed several abnormalities, such as the appearance of univalents and an increase in rod-shaped bivalents. Based on our findings from the morphological observations and crossing experiments, we conclude that each of three identified types represents a distinct species. We propose that the large-type is F. cancrivora, the mangrove-type is F. moodiei, and the Sulawesi-type represents an undescribed species. Zoological Society of Japan 2011 Article PeerReviewed Kurniawan, N. and Djong, T.H. and Islam, M.M. and Nishizawa, T. and Belabut, D.M. and Yong, Hoi Sen and Yasir, I. and Sumida, M. (2011) Taxonomic Status of Three Types of Fejervarya cancrivora from Indonesia and Other Asian Countries Based on Morphological Observations and Crossing Experiments. Zoological Science, 28 (1). pp. 12-24. ISSN 0289-0003, DOI https://doi.org/10.2108/zsj.28.12 <https://doi.org/10.2108/zsj.28.12>. http://www.bioone.org/doi/abs/10.2108/zsj.28.12?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed&#aff1 http://dx.doi.org/10.2108/zsj.28.12 |
| spellingShingle | Q Science (General) QH Natural history Kurniawan, N. Djong, T.H. Islam, M.M. Nishizawa, T. Belabut, D.M. Yong, Hoi Sen Yasir, I. Sumida, M. Taxonomic Status of Three Types of Fejervarya cancrivora from Indonesia and Other Asian Countries Based on Morphological Observations and Crossing Experiments |
| title | Taxonomic Status of Three Types of Fejervarya cancrivora from Indonesia and Other Asian Countries Based on Morphological Observations and Crossing Experiments |
| title_full | Taxonomic Status of Three Types of Fejervarya cancrivora from Indonesia and Other Asian Countries Based on Morphological Observations and Crossing Experiments |
| title_fullStr | Taxonomic Status of Three Types of Fejervarya cancrivora from Indonesia and Other Asian Countries Based on Morphological Observations and Crossing Experiments |
| title_full_unstemmed | Taxonomic Status of Three Types of Fejervarya cancrivora from Indonesia and Other Asian Countries Based on Morphological Observations and Crossing Experiments |
| title_short | Taxonomic Status of Three Types of Fejervarya cancrivora from Indonesia and Other Asian Countries Based on Morphological Observations and Crossing Experiments |
| title_sort | taxonomic status of three types of fejervarya cancrivora from indonesia and other asian countries based on morphological observations and crossing experiments |
| topic | Q Science (General) QH Natural history |
| work_keys_str_mv | AT kurniawann taxonomicstatusofthreetypesoffejervaryacancrivorafromindonesiaandotherasiancountriesbasedonmorphologicalobservationsandcrossingexperiments AT djongth taxonomicstatusofthreetypesoffejervaryacancrivorafromindonesiaandotherasiancountriesbasedonmorphologicalobservationsandcrossingexperiments AT islammm taxonomicstatusofthreetypesoffejervaryacancrivorafromindonesiaandotherasiancountriesbasedonmorphologicalobservationsandcrossingexperiments AT nishizawat taxonomicstatusofthreetypesoffejervaryacancrivorafromindonesiaandotherasiancountriesbasedonmorphologicalobservationsandcrossingexperiments AT belabutdm taxonomicstatusofthreetypesoffejervaryacancrivorafromindonesiaandotherasiancountriesbasedonmorphologicalobservationsandcrossingexperiments AT yonghoisen taxonomicstatusofthreetypesoffejervaryacancrivorafromindonesiaandotherasiancountriesbasedonmorphologicalobservationsandcrossingexperiments AT yasiri taxonomicstatusofthreetypesoffejervaryacancrivorafromindonesiaandotherasiancountriesbasedonmorphologicalobservationsandcrossingexperiments AT sumidam taxonomicstatusofthreetypesoffejervaryacancrivorafromindonesiaandotherasiancountriesbasedonmorphologicalobservationsandcrossingexperiments |