Investigation of extraction and transesterification of algae by immobilized lipase as biofuel
In this study, three different methods were investigated to study the disruption of algae cells walls such as ultrasonication, soxhlet and hexane (solvent) extraction. Chlorella sp. and Spirulina sp. were two selected algae species to analyze the effect of each method for biodiesel production. The o...
| Main Authors: | , , , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2015
|
| Subjects: | |
| Online Access: | http://eprints.um.edu.my/13878/1/0001.pdf |
| _version_ | 1796946652157706240 |
|---|---|
| author | Mohd Yusof, Noraini Ong, Hwai Chyuan Jan, Badrul Mohamed Chong, Wen Tong Masjuki, Haji Hassan |
| author_facet | Mohd Yusof, Noraini Ong, Hwai Chyuan Jan, Badrul Mohamed Chong, Wen Tong Masjuki, Haji Hassan |
| author_sort | Mohd Yusof, Noraini |
| collection | UM |
| description | In this study, three different methods were investigated to study the disruption of algae cells walls such as ultrasonication, soxhlet and hexane (solvent) extraction. Chlorella sp. and Spirulina sp. were two selected algae species to analyze the effect of each method for biodiesel production. The oil yield is calculated to determine the best effective method for oil extraction. The highest oil yield obtained by ultrasonication followed by Soxhlet and hexane extraction. Moreover, acid value for Chlorella is the lowest for all extraction method. Then, the crude algae oil was transesterified by Novozyme 435, an immobilized enzyme, at 60°C, 300 rpm for 24 hours to hydrolyze triglycerides into free fatty acids. The ester content was analyzed by FTIR and found at 1735, 1731, 1214, 1162, 1161, 1064, 1060, 1059 cm-1 frequency. Fatty acid derivatives were measured by gas chromatography and palmitic acid was among ofthe major component in microalgae biodiesel. |
| first_indexed | 2024-03-06T05:35:02Z |
| format | Conference or Workshop Item |
| id | um.eprints-13878 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:35:02Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | um.eprints-138782018-10-18T01:06:27Z http://eprints.um.edu.my/13878/ Investigation of extraction and transesterification of algae by immobilized lipase as biofuel Mohd Yusof, Noraini Ong, Hwai Chyuan Jan, Badrul Mohamed Chong, Wen Tong Masjuki, Haji Hassan TJ Mechanical engineering and machinery TP Chemical technology In this study, three different methods were investigated to study the disruption of algae cells walls such as ultrasonication, soxhlet and hexane (solvent) extraction. Chlorella sp. and Spirulina sp. were two selected algae species to analyze the effect of each method for biodiesel production. The oil yield is calculated to determine the best effective method for oil extraction. The highest oil yield obtained by ultrasonication followed by Soxhlet and hexane extraction. Moreover, acid value for Chlorella is the lowest for all extraction method. Then, the crude algae oil was transesterified by Novozyme 435, an immobilized enzyme, at 60°C, 300 rpm for 24 hours to hydrolyze triglycerides into free fatty acids. The ester content was analyzed by FTIR and found at 1735, 1731, 1214, 1162, 1161, 1064, 1060, 1059 cm-1 frequency. Fatty acid derivatives were measured by gas chromatography and palmitic acid was among ofthe major component in microalgae biodiesel. 2015-06 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.um.edu.my/13878/1/0001.pdf Mohd Yusof, Noraini and Ong, Hwai Chyuan and Jan, Badrul Mohamed and Chong, Wen Tong and Masjuki, Haji Hassan (2015) Investigation of extraction and transesterification of algae by immobilized lipase as biofuel. In: 5th International Conference on Algal Biomass, Biofuels & Bioproducts, 7-10 June 2015, San Diego, USA. (Submitted) |
| spellingShingle | TJ Mechanical engineering and machinery TP Chemical technology Mohd Yusof, Noraini Ong, Hwai Chyuan Jan, Badrul Mohamed Chong, Wen Tong Masjuki, Haji Hassan Investigation of extraction and transesterification of algae by immobilized lipase as biofuel |
| title | Investigation of extraction and transesterification of algae by immobilized lipase as biofuel
|
| title_full | Investigation of extraction and transesterification of algae by immobilized lipase as biofuel
|
| title_fullStr | Investigation of extraction and transesterification of algae by immobilized lipase as biofuel
|
| title_full_unstemmed | Investigation of extraction and transesterification of algae by immobilized lipase as biofuel
|
| title_short | Investigation of extraction and transesterification of algae by immobilized lipase as biofuel
|
| title_sort | investigation of extraction and transesterification of algae by immobilized lipase as biofuel |
| topic | TJ Mechanical engineering and machinery TP Chemical technology |
| url | http://eprints.um.edu.my/13878/1/0001.pdf |
| work_keys_str_mv | AT mohdyusofnoraini investigationofextractionandtransesterificationofalgaebyimmobilizedlipaseasbiofuel AT onghwaichyuan investigationofextractionandtransesterificationofalgaebyimmobilizedlipaseasbiofuel AT janbadrulmohamed investigationofextractionandtransesterificationofalgaebyimmobilizedlipaseasbiofuel AT chongwentong investigationofextractionandtransesterificationofalgaebyimmobilizedlipaseasbiofuel AT masjukihajihassan investigationofextractionandtransesterificationofalgaebyimmobilizedlipaseasbiofuel |