Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells
Background: Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction. Methods: In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfolia...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BioMed Central
2015
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| Online Access: | http://eprints.um.edu.my/14035/1/s12906-015-0749-6.pdf |
| _version_ | 1825720460141133824 |
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| author | Aziz, J. Abu Kassim, N.L. Abu Kasim, N.H. Haque, N. Rahman, M.R. |
| author_facet | Aziz, J. Abu Kassim, N.L. Abu Kasim, N.H. Haque, N. Rahman, M.R. |
| author_sort | Aziz, J. |
| collection | UM |
| description | Background: Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction. Methods: In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papaya pulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF). Results: In vitro scratch gap closure was significantly faster (p < .05) in SHED culture treated with UPJ. IL-6 concentration was significantly increased (p < .05) in SHED and PBL culture supernatant when treated with UPJ. SCF synthesis in SHED culture was also significantly increased (p < .05) when treated with UPJ. Conclusion: In vitro upregulated synthesis of IL-6 and SCF both in PBL and SHED reveals the potential mechanism of unripe papaya to induce thrombopoietic cytokines synthesis in cells of hematopoietic and mesenchymal origin. |
| first_indexed | 2024-03-06T05:35:29Z |
| format | Article |
| id | um.eprints-14035 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:35:29Z |
| publishDate | 2015 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | um.eprints-140352015-09-22T01:09:13Z http://eprints.um.edu.my/14035/ Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells Aziz, J. Abu Kassim, N.L. Abu Kasim, N.H. Haque, N. Rahman, M.R. RK Dentistry Background: Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction. Methods: In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papaya pulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF). Results: In vitro scratch gap closure was significantly faster (p < .05) in SHED culture treated with UPJ. IL-6 concentration was significantly increased (p < .05) in SHED and PBL culture supernatant when treated with UPJ. SCF synthesis in SHED culture was also significantly increased (p < .05) when treated with UPJ. Conclusion: In vitro upregulated synthesis of IL-6 and SCF both in PBL and SHED reveals the potential mechanism of unripe papaya to induce thrombopoietic cytokines synthesis in cells of hematopoietic and mesenchymal origin. BioMed Central 2015 Article PeerReviewed application/pdf en http://eprints.um.edu.my/14035/1/s12906-015-0749-6.pdf Aziz, J. and Abu Kassim, N.L. and Abu Kasim, N.H. and Haque, N. and Rahman, M.R. (2015) Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells. BMC Complementary and Alternative Medicine, 15 (215). ISSN 1472-6882, DOI https://doi.org/10.1186/s12906-015-0749-6 <https://doi.org/10.1186/s12906-015-0749-6>. http://www.biomedcentral.com/1472-6882/15/215 10.1186/s12906-015-0749-6 |
| spellingShingle | RK Dentistry Aziz, J. Abu Kassim, N.L. Abu Kasim, N.H. Haque, N. Rahman, M.R. Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells |
| title | Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells |
| title_full | Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells |
| title_fullStr | Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells |
| title_full_unstemmed | Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells |
| title_short | Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells |
| title_sort | carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells |
| topic | RK Dentistry |
| url | http://eprints.um.edu.my/14035/1/s12906-015-0749-6.pdf |
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