Quantification of Mesenchymal Stem Cell Growth Rates through Secretory and Excretory Biomolecules in Conditioned Media via Fresnel Reflection
An efficient and low cost optical method for directly measuring the concentration of homogenous biological solutes is proposed and demonstrated. The proposed system operates by Fresnel reflection, with a flat-cleaved single-mode fiber serving as the sensor probe. A laser provides a 12.9 dBm sensor s...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
MDPI
2013
|
| Subjects: |
| _version_ | 1825720513458077696 |
|---|---|
| author | Ahmad, Harith Thambiratnam, K. Zulkifli, Ahmad Zarif Lawrence, A. Jasim, A.A. Kunasekaran, W. Musa, S. Gnanasegaran, N. Vasanthan, P. Jayaraman, P. Kasim, N.H.A. Govindasamy, V. Shahrir, M.S. Harun, Sulaiman Wadi |
| author_facet | Ahmad, Harith Thambiratnam, K. Zulkifli, Ahmad Zarif Lawrence, A. Jasim, A.A. Kunasekaran, W. Musa, S. Gnanasegaran, N. Vasanthan, P. Jayaraman, P. Kasim, N.H.A. Govindasamy, V. Shahrir, M.S. Harun, Sulaiman Wadi |
| author_sort | Ahmad, Harith |
| collection | UM |
| description | An efficient and low cost optical method for directly measuring the concentration of homogenous biological solutes is proposed and demonstrated. The proposed system operates by Fresnel reflection, with a flat-cleaved single-mode fiber serving as the sensor probe. A laser provides a 12.9 dBm sensor signal at 1,550 nm, while a computer-controlled optical power meter measures the power of the signal returned by the probe. Three different mesenchymal stem cell (MSC) lines were obtained, sub-cultured and trypsinized daily over 9 days. Counts were measured using a haemocytometer and the conditioned media (CM) was collected daily and stored at −80 °C. MSCs release excretory biomolecules proportional to their growth rate into the CM, which changes the refractive index of the latter. The sensor is capable of detecting changes in the number of stem cells via correlation to the change in the refractive index of the CM, with the measured power loss decreasing approximately 0.4 dB in the CM sample per average 1,000 cells in the MSC subculture. The proposed system is highly cost-effective, simple to deploy, operate, and maintain, is non-destructive, and allows reliable real-time measurement of various stem cell proliferation parameters. |
| first_indexed | 2024-03-06T05:36:18Z |
| format | Article |
| id | um.eprints-14387 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:36:18Z |
| publishDate | 2013 |
| publisher | MDPI |
| record_format | dspace |
| spelling | um.eprints-143872019-12-20T07:11:39Z http://eprints.um.edu.my/14387/ Quantification of Mesenchymal Stem Cell Growth Rates through Secretory and Excretory Biomolecules in Conditioned Media via Fresnel Reflection Ahmad, Harith Thambiratnam, K. Zulkifli, Ahmad Zarif Lawrence, A. Jasim, A.A. Kunasekaran, W. Musa, S. Gnanasegaran, N. Vasanthan, P. Jayaraman, P. Kasim, N.H.A. Govindasamy, V. Shahrir, M.S. Harun, Sulaiman Wadi Q Science (General) RK Dentistry An efficient and low cost optical method for directly measuring the concentration of homogenous biological solutes is proposed and demonstrated. The proposed system operates by Fresnel reflection, with a flat-cleaved single-mode fiber serving as the sensor probe. A laser provides a 12.9 dBm sensor signal at 1,550 nm, while a computer-controlled optical power meter measures the power of the signal returned by the probe. Three different mesenchymal stem cell (MSC) lines were obtained, sub-cultured and trypsinized daily over 9 days. Counts were measured using a haemocytometer and the conditioned media (CM) was collected daily and stored at −80 °C. MSCs release excretory biomolecules proportional to their growth rate into the CM, which changes the refractive index of the latter. The sensor is capable of detecting changes in the number of stem cells via correlation to the change in the refractive index of the CM, with the measured power loss decreasing approximately 0.4 dB in the CM sample per average 1,000 cells in the MSC subculture. The proposed system is highly cost-effective, simple to deploy, operate, and maintain, is non-destructive, and allows reliable real-time measurement of various stem cell proliferation parameters. MDPI 2013 Article PeerReviewed Ahmad, Harith and Thambiratnam, K. and Zulkifli, Ahmad Zarif and Lawrence, A. and Jasim, A.A. and Kunasekaran, W. and Musa, S. and Gnanasegaran, N. and Vasanthan, P. and Jayaraman, P. and Kasim, N.H.A. and Govindasamy, V. and Shahrir, M.S. and Harun, Sulaiman Wadi (2013) Quantification of Mesenchymal Stem Cell Growth Rates through Secretory and Excretory Biomolecules in Conditioned Media via Fresnel Reflection. Sensors, 13 (10). pp. 13276-13288. ISSN 1424-8220, DOI https://doi.org/10.3390/s131013276 <https://doi.org/10.3390/s131013276>. http://www.mdpi.com/1424-8220/13/10/13276 doi:10.3390/s131013276 |
| spellingShingle | Q Science (General) RK Dentistry Ahmad, Harith Thambiratnam, K. Zulkifli, Ahmad Zarif Lawrence, A. Jasim, A.A. Kunasekaran, W. Musa, S. Gnanasegaran, N. Vasanthan, P. Jayaraman, P. Kasim, N.H.A. Govindasamy, V. Shahrir, M.S. Harun, Sulaiman Wadi Quantification of Mesenchymal Stem Cell Growth Rates through Secretory and Excretory Biomolecules in Conditioned Media via Fresnel Reflection |
| title | Quantification of Mesenchymal Stem Cell Growth Rates through Secretory and Excretory Biomolecules in Conditioned Media via Fresnel Reflection |
| title_full | Quantification of Mesenchymal Stem Cell Growth Rates through Secretory and Excretory Biomolecules in Conditioned Media via Fresnel Reflection |
| title_fullStr | Quantification of Mesenchymal Stem Cell Growth Rates through Secretory and Excretory Biomolecules in Conditioned Media via Fresnel Reflection |
| title_full_unstemmed | Quantification of Mesenchymal Stem Cell Growth Rates through Secretory and Excretory Biomolecules in Conditioned Media via Fresnel Reflection |
| title_short | Quantification of Mesenchymal Stem Cell Growth Rates through Secretory and Excretory Biomolecules in Conditioned Media via Fresnel Reflection |
| title_sort | quantification of mesenchymal stem cell growth rates through secretory and excretory biomolecules in conditioned media via fresnel reflection |
| topic | Q Science (General) RK Dentistry |
| work_keys_str_mv | AT ahmadharith quantificationofmesenchymalstemcellgrowthratesthroughsecretoryandexcretorybiomoleculesinconditionedmediaviafresnelreflection AT thambiratnamk quantificationofmesenchymalstemcellgrowthratesthroughsecretoryandexcretorybiomoleculesinconditionedmediaviafresnelreflection AT zulkifliahmadzarif quantificationofmesenchymalstemcellgrowthratesthroughsecretoryandexcretorybiomoleculesinconditionedmediaviafresnelreflection AT lawrencea quantificationofmesenchymalstemcellgrowthratesthroughsecretoryandexcretorybiomoleculesinconditionedmediaviafresnelreflection AT jasimaa quantificationofmesenchymalstemcellgrowthratesthroughsecretoryandexcretorybiomoleculesinconditionedmediaviafresnelreflection AT kunasekaranw quantificationofmesenchymalstemcellgrowthratesthroughsecretoryandexcretorybiomoleculesinconditionedmediaviafresnelreflection AT musas quantificationofmesenchymalstemcellgrowthratesthroughsecretoryandexcretorybiomoleculesinconditionedmediaviafresnelreflection AT gnanasegarann quantificationofmesenchymalstemcellgrowthratesthroughsecretoryandexcretorybiomoleculesinconditionedmediaviafresnelreflection AT vasanthanp quantificationofmesenchymalstemcellgrowthratesthroughsecretoryandexcretorybiomoleculesinconditionedmediaviafresnelreflection AT jayaramanp quantificationofmesenchymalstemcellgrowthratesthroughsecretoryandexcretorybiomoleculesinconditionedmediaviafresnelreflection AT kasimnha quantificationofmesenchymalstemcellgrowthratesthroughsecretoryandexcretorybiomoleculesinconditionedmediaviafresnelreflection AT govindasamyv quantificationofmesenchymalstemcellgrowthratesthroughsecretoryandexcretorybiomoleculesinconditionedmediaviafresnelreflection AT shahrirms quantificationofmesenchymalstemcellgrowthratesthroughsecretoryandexcretorybiomoleculesinconditionedmediaviafresnelreflection AT harunsulaimanwadi quantificationofmesenchymalstemcellgrowthratesthroughsecretoryandexcretorybiomoleculesinconditionedmediaviafresnelreflection |