Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression

Candida albicans is an opportunistic human pathogen with the ability to differentiate and grow in filamentous forms and exist as biofilms. The biofilms are a barrier to treatment as they are often resistant to the antifungal drugs. In this study, we investigated the antifungal activity of allicin, a...

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Main Authors: Khodavandi, A., Harmal, N.S., Alizadeh, F., Scully, O.J., Sidik, S.M., Othman, Fauziah, Sekawi, Z., Ng, Kee Peng, Chong, P.P.
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Published: Elsevier 2011
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author Khodavandi, A.
Harmal, N.S.
Alizadeh, F.
Scully, O.J.
Sidik, S.M.
Othman, Fauziah
Sekawi, Z.
Ng, Kee Peng
Chong, P.P.
author_facet Khodavandi, A.
Harmal, N.S.
Alizadeh, F.
Scully, O.J.
Sidik, S.M.
Othman, Fauziah
Sekawi, Z.
Ng, Kee Peng
Chong, P.P.
author_sort Khodavandi, A.
collection UM
description Candida albicans is an opportunistic human pathogen with the ability to differentiate and grow in filamentous forms and exist as biofilms. The biofilms are a barrier to treatment as they are often resistant to the antifungal drugs. In this study, we investigated the antifungal activity of allicin, an active compound of garlic on various isolates of C. albicans. The effect of allicin on biofilm production in C. albicans as compared to fluconazole, an antifungal drug, was investigated using the tetrazolium (XXT) reduction-dependent growth and crystal violet assays as well as scanning electron microscopy (SEM). Allicin-treated cells exhibited significant reduction in biofilm growth (p<0.05) compared to fluconazole-treated and also growth control cells. Moreover, observation by SEM of allicin and fluconazole-treated cells confirmed a dose-dependent membrane disruption and decreased production of organisms. Finally, the expression of selected genes involved in biofilm formation such as HWP1 was evaluated by semi-quantitative RT-PCR and relative real time RT-PCR. Allicin was shown to down-regulate the expression of HWP1. (C) 2011 Elsevier GmbH. All rights reserved.
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spelling um.eprints-145412019-12-23T08:06:51Z http://eprints.um.edu.my/14541/ Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression Khodavandi, A. Harmal, N.S. Alizadeh, F. Scully, O.J. Sidik, S.M. Othman, Fauziah Sekawi, Z. Ng, Kee Peng Chong, P.P. Q Science (General) R Medicine Candida albicans is an opportunistic human pathogen with the ability to differentiate and grow in filamentous forms and exist as biofilms. The biofilms are a barrier to treatment as they are often resistant to the antifungal drugs. In this study, we investigated the antifungal activity of allicin, an active compound of garlic on various isolates of C. albicans. The effect of allicin on biofilm production in C. albicans as compared to fluconazole, an antifungal drug, was investigated using the tetrazolium (XXT) reduction-dependent growth and crystal violet assays as well as scanning electron microscopy (SEM). Allicin-treated cells exhibited significant reduction in biofilm growth (p<0.05) compared to fluconazole-treated and also growth control cells. Moreover, observation by SEM of allicin and fluconazole-treated cells confirmed a dose-dependent membrane disruption and decreased production of organisms. Finally, the expression of selected genes involved in biofilm formation such as HWP1 was evaluated by semi-quantitative RT-PCR and relative real time RT-PCR. Allicin was shown to down-regulate the expression of HWP1. (C) 2011 Elsevier GmbH. All rights reserved. Elsevier 2011 Article PeerReviewed Khodavandi, A. and Harmal, N.S. and Alizadeh, F. and Scully, O.J. and Sidik, S.M. and Othman, Fauziah and Sekawi, Z. and Ng, Kee Peng and Chong, P.P. (2011) Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression. Phytomedicine, 19 (1). pp. 56-63. https://doi.org/10.1016/j.phymed.2011.08.060
spellingShingle Q Science (General)
R Medicine
Khodavandi, A.
Harmal, N.S.
Alizadeh, F.
Scully, O.J.
Sidik, S.M.
Othman, Fauziah
Sekawi, Z.
Ng, Kee Peng
Chong, P.P.
Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression
title Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression
title_full Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression
title_fullStr Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression
title_full_unstemmed Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression
title_short Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression
title_sort comparison between allicin and fluconazole in candida albicans biofilm inhibition and in suppression of hwp1 gene expression
topic Q Science (General)
R Medicine
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