Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture
The possibility of using in situ addition of anion-exchange resin for the removal of acetate in the culture aimed at improving growth of E. coli and expression of periplasmic human interferon-alpha ab (PrIFN-alpha 2b) was studied in shake flask culture and stirred tank bioreactor. Different types of...
| Hoofdauteurs: | , , , , |
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| Formaat: | Artikel |
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Elsevier
2011
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| Onderwerpen: |
| _version_ | 1825720543022678016 |
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| author | Tan, J.S. Ramanan, R.N. Ling, T.C. Shuhaimi, M. Ariff, A.B. |
| author_facet | Tan, J.S. Ramanan, R.N. Ling, T.C. Shuhaimi, M. Ariff, A.B. |
| author_sort | Tan, J.S. |
| collection | UM |
| description | The possibility of using in situ addition of anion-exchange resin for the removal of acetate in the culture aimed at improving growth of E. coli and expression of periplasmic human interferon-alpha ab (PrIFN-alpha 2b) was studied in shake flask culture and stirred tank bioreactor. Different types of anion-exchange resin were evaluated and the concentration of anion-exchange resin was optimized using response surface methodology. The addition of anion-exchange resins reduced acetate accumulation in the culture, which in turn, improved growth of E. coli and enhanced PrIFN-alpha 2b expression. The presence of anion-exchange resins did not influence the physiology of the cells. The weak base anion-exchange resins, which have higher affinity towards acetate, yielded higher PrIFN-alpha 2b expression as compared to strong anion-exchange resins. High concentrations of anion-exchange resin showed inhibitory effect towards growth of E. coli as well as the expression of PrIFN-alpha 2b. The maximum yield of PrIFN-alpha 2b in shake flask culture (501.8 mu g/L) and stirred tank bioreactor (578.8 mu g/L) was obtained at ion exchange resin (WA 30) concentration of 12.2 g/L. The production of PrIFN-alpha 2b in stirred tank bioreactor with the addition of ion exchange resin was about 1.8-fold higher than that obtained in fermentation without ion exchange resin (318.4 mu g/L). (C) 2011 Elsevier By. All rights reserved. |
| first_indexed | 2024-03-06T05:36:43Z |
| format | Article |
| id | um.eprints-14545 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:36:43Z |
| publishDate | 2011 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | um.eprints-145452015-11-05T10:53:01Z http://eprints.um.edu.my/14545/ Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture Tan, J.S. Ramanan, R.N. Ling, T.C. Shuhaimi, M. Ariff, A.B. Q Science (General) The possibility of using in situ addition of anion-exchange resin for the removal of acetate in the culture aimed at improving growth of E. coli and expression of periplasmic human interferon-alpha ab (PrIFN-alpha 2b) was studied in shake flask culture and stirred tank bioreactor. Different types of anion-exchange resin were evaluated and the concentration of anion-exchange resin was optimized using response surface methodology. The addition of anion-exchange resins reduced acetate accumulation in the culture, which in turn, improved growth of E. coli and enhanced PrIFN-alpha 2b expression. The presence of anion-exchange resins did not influence the physiology of the cells. The weak base anion-exchange resins, which have higher affinity towards acetate, yielded higher PrIFN-alpha 2b expression as compared to strong anion-exchange resins. High concentrations of anion-exchange resin showed inhibitory effect towards growth of E. coli as well as the expression of PrIFN-alpha 2b. The maximum yield of PrIFN-alpha 2b in shake flask culture (501.8 mu g/L) and stirred tank bioreactor (578.8 mu g/L) was obtained at ion exchange resin (WA 30) concentration of 12.2 g/L. The production of PrIFN-alpha 2b in stirred tank bioreactor with the addition of ion exchange resin was about 1.8-fold higher than that obtained in fermentation without ion exchange resin (318.4 mu g/L). (C) 2011 Elsevier By. All rights reserved. Elsevier 2011 Article PeerReviewed Tan, J.S. and Ramanan, R.N. and Ling, T.C. and Shuhaimi, M. and Ariff, A.B. (2011) Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture. Biochemical Engineering Journal, 58-59. pp. 124-132. |
| spellingShingle | Q Science (General) Tan, J.S. Ramanan, R.N. Ling, T.C. Shuhaimi, M. Ariff, A.B. Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture |
| title | Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture |
| title_full | Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture |
| title_fullStr | Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture |
| title_full_unstemmed | Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture |
| title_short | Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture |
| title_sort | enhanced production of periplasmic interferon alpha 2b by escherichia coli using ion exchange resin for in situ removal of acetate in the culture |
| topic | Q Science (General) |
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