Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture
The possibility of using in situ addition of anion-exchange resin for the removal of acetate in the culture aimed at improving growth of E. coli and expression of periplasmic human interferon-alpha ab (PrIFN-alpha 2b) was studied in shake flask culture and stirred tank bioreactor. Different types of...
Main Authors: | Tan, J.S., Ramanan, R.N., Ling, T.C., Shuhaimi, M., Ariff, A.B. |
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Format: | Article |
Published: |
Elsevier
2011
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Subjects: |
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