Using SERS-based microfluidic paper-based device (μPAD) for calibration-free quantitative measurement of AMI cardiac biomarkers
Recently, surface enhanced Raman scattering (SERS) has attracted much attention in medical diagnosis applications owing to better detection sensitivity and lower limit of detection (LOD) than colorimetric detection. In this paper, a novel calibration-free SERS−based μPAD with multi-reaction zones fo...
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Elsevier
2020
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author | Lim, Wei Yin Goh, Choon Hian Thevarajah, T. Malathi Goh, Boon Tong Khor, Sook Mei |
author_facet | Lim, Wei Yin Goh, Choon Hian Thevarajah, T. Malathi Goh, Boon Tong Khor, Sook Mei |
author_sort | Lim, Wei Yin |
collection | UM |
description | Recently, surface enhanced Raman scattering (SERS) has attracted much attention in medical diagnosis applications owing to better detection sensitivity and lower limit of detection (LOD) than colorimetric detection. In this paper, a novel calibration-free SERS−based μPAD with multi-reaction zones for simultaneous quantitative detection of multiple cardiac biomarkers — GPBB, CK−MB and cTnT for early diagnosis and prognosis of acute myocardial infarction (AMI) are presented. Three distinct Raman probes were synthesised, subsequently conjugated with respective detecting antibodies and used as SERS nanotags for cardiac biomarker detection. Using a conventional calibration curve, quantitative simultaneous measurement of multiple cardiac biomarkers on SERS-based μPAD was performed based on the characteristic Raman spectral features of each reporter used in different nanotags. However, a calibration free point-of-care testing device is required for fast screening to rule-in and rule-out AMI patients. Partial least squares predictive models were developed and incorporated into the immunosensing system, to accurately quantify the three unknown cardiac biomarkers levels in serum based on the previously obtained Raman spectral data. This method allows absolute quantitative measurement when conventional calibration curve fails to provide accurate estimation of cardiac biomarkers, especially at low and high concentration ranges. Under an optimised condition, the LOD of our SERS-based μPAD was identified at 8, 10, and 1 pg mL−1, for GPBB, CK−MB and cTnT, respectively, which is well below the clinical cutoff values. Therefore, this proof-of-concept technique shows significant potential for highly sensitive quantitative detection of multiplex cardiac biomarkers in human serum to expedite medical decisions for enhanced patient care. © 2019 Elsevier B.V. |
first_indexed | 2024-03-06T05:37:14Z |
format | Article |
id | um.eprints-14767 |
institution | Universiti Malaya |
last_indexed | 2024-03-06T05:37:14Z |
publishDate | 2020 |
publisher | Elsevier |
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spelling | um.eprints-147672019-11-11T04:45:27Z http://eprints.um.edu.my/14767/ Using SERS-based microfluidic paper-based device (μPAD) for calibration-free quantitative measurement of AMI cardiac biomarkers Lim, Wei Yin Goh, Choon Hian Thevarajah, T. Malathi Goh, Boon Tong Khor, Sook Mei QC Physics QD Chemistry R Medicine Recently, surface enhanced Raman scattering (SERS) has attracted much attention in medical diagnosis applications owing to better detection sensitivity and lower limit of detection (LOD) than colorimetric detection. In this paper, a novel calibration-free SERS−based μPAD with multi-reaction zones for simultaneous quantitative detection of multiple cardiac biomarkers — GPBB, CK−MB and cTnT for early diagnosis and prognosis of acute myocardial infarction (AMI) are presented. Three distinct Raman probes were synthesised, subsequently conjugated with respective detecting antibodies and used as SERS nanotags for cardiac biomarker detection. Using a conventional calibration curve, quantitative simultaneous measurement of multiple cardiac biomarkers on SERS-based μPAD was performed based on the characteristic Raman spectral features of each reporter used in different nanotags. However, a calibration free point-of-care testing device is required for fast screening to rule-in and rule-out AMI patients. Partial least squares predictive models were developed and incorporated into the immunosensing system, to accurately quantify the three unknown cardiac biomarkers levels in serum based on the previously obtained Raman spectral data. This method allows absolute quantitative measurement when conventional calibration curve fails to provide accurate estimation of cardiac biomarkers, especially at low and high concentration ranges. Under an optimised condition, the LOD of our SERS-based μPAD was identified at 8, 10, and 1 pg mL−1, for GPBB, CK−MB and cTnT, respectively, which is well below the clinical cutoff values. Therefore, this proof-of-concept technique shows significant potential for highly sensitive quantitative detection of multiplex cardiac biomarkers in human serum to expedite medical decisions for enhanced patient care. © 2019 Elsevier B.V. Elsevier 2020 Article PeerReviewed Lim, Wei Yin and Goh, Choon Hian and Thevarajah, T. Malathi and Goh, Boon Tong and Khor, Sook Mei (2020) Using SERS-based microfluidic paper-based device (μPAD) for calibration-free quantitative measurement of AMI cardiac biomarkers. Biosensors and Bioelectronics, 147. p. 111792. ISSN 0956-5663, DOI https://doi.org/10.1016/j.bios.2019.111792 <https://doi.org/10.1016/j.bios.2019.111792>. https://doi.org/10.1016/j.bios.2019.111792 doi:10.1016/j.bios.2019.111792 |
spellingShingle | QC Physics QD Chemistry R Medicine Lim, Wei Yin Goh, Choon Hian Thevarajah, T. Malathi Goh, Boon Tong Khor, Sook Mei Using SERS-based microfluidic paper-based device (μPAD) for calibration-free quantitative measurement of AMI cardiac biomarkers |
title | Using SERS-based microfluidic paper-based device (μPAD) for calibration-free quantitative measurement of AMI cardiac biomarkers |
title_full | Using SERS-based microfluidic paper-based device (μPAD) for calibration-free quantitative measurement of AMI cardiac biomarkers |
title_fullStr | Using SERS-based microfluidic paper-based device (μPAD) for calibration-free quantitative measurement of AMI cardiac biomarkers |
title_full_unstemmed | Using SERS-based microfluidic paper-based device (μPAD) for calibration-free quantitative measurement of AMI cardiac biomarkers |
title_short | Using SERS-based microfluidic paper-based device (μPAD) for calibration-free quantitative measurement of AMI cardiac biomarkers |
title_sort | using sers based microfluidic paper based device μpad for calibration free quantitative measurement of ami cardiac biomarkers |
topic | QC Physics QD Chemistry R Medicine |
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