Chemopreventive properties of phytosterols and maslinic acid extracted from coleus tuberosus in inhibiting the expression of EBV early-antigen in raji cells
Bioassay-guided fractionation of a MeOH extract of tubers of Coleus tuberosus afforded the active anti-tumor-promoting compounds identified as the triterpenoid 2 alpha,3 beta-dihydroxyolean-12-en-28-oic acid (maslinic acid; CT2) and a phytosterol mixture (CT1). CT1 consists of stigmasterol (32%), be...
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| Format: | Article |
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John Wiley & Sons
2010
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| _version_ | 1825720670681563136 |
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| author | Mooi, L.Y. Wahab, N.A. Lajis, N. Ali, A.M. |
| author_facet | Mooi, L.Y. Wahab, N.A. Lajis, N. Ali, A.M. |
| author_sort | Mooi, L.Y. |
| collection | UM |
| description | Bioassay-guided fractionation of a MeOH extract of tubers of Coleus tuberosus afforded the active anti-tumor-promoting compounds identified as the triterpenoid 2 alpha,3 beta-dihydroxyolean-12-en-28-oic acid (maslinic acid; CT2) and a phytosterol mixture (CT1). CT1 consists of stigmasterol (32%), beta-sitosterol (40.3%), and campesterol (27.7%) as determined by capillary gas chromatography. CT1 and CT2 showed very strong anti-tumor-promoting activities at IC(50) 0.7 mu g/ml and 0.1 mu g/ml, respectively, in a convenient, short-term in vitro assay, i.e., the inhibition of Epstein Barr virus (EBV) activation induced by phorbol 12-myristate 13-acetate (PMA) and sodium butyrate. We report for the first time the anti-tumor-promoting activity of 2 alpha,3 beta-dihydroxyolean-12-en-28-oic acid and show that a mixture of stigmasterol, beta-sitosterol, and campesterol is more potent than the individual components in inhibiting tumor-promoting activity. |
| first_indexed | 2024-03-06T05:38:39Z |
| format | Article |
| id | um.eprints-15358 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:38:39Z |
| publishDate | 2010 |
| publisher | John Wiley & Sons |
| record_format | dspace |
| spelling | um.eprints-153582015-12-28T12:50:05Z http://eprints.um.edu.my/15358/ Chemopreventive properties of phytosterols and maslinic acid extracted from coleus tuberosus in inhibiting the expression of EBV early-antigen in raji cells Mooi, L.Y. Wahab, N.A. Lajis, N. Ali, A.M. Q Science (General) Bioassay-guided fractionation of a MeOH extract of tubers of Coleus tuberosus afforded the active anti-tumor-promoting compounds identified as the triterpenoid 2 alpha,3 beta-dihydroxyolean-12-en-28-oic acid (maslinic acid; CT2) and a phytosterol mixture (CT1). CT1 consists of stigmasterol (32%), beta-sitosterol (40.3%), and campesterol (27.7%) as determined by capillary gas chromatography. CT1 and CT2 showed very strong anti-tumor-promoting activities at IC(50) 0.7 mu g/ml and 0.1 mu g/ml, respectively, in a convenient, short-term in vitro assay, i.e., the inhibition of Epstein Barr virus (EBV) activation induced by phorbol 12-myristate 13-acetate (PMA) and sodium butyrate. We report for the first time the anti-tumor-promoting activity of 2 alpha,3 beta-dihydroxyolean-12-en-28-oic acid and show that a mixture of stigmasterol, beta-sitosterol, and campesterol is more potent than the individual components in inhibiting tumor-promoting activity. John Wiley & Sons 2010 Article PeerReviewed Mooi, L.Y. and Wahab, N.A. and Lajis, N. and Ali, A.M. (2010) Chemopreventive properties of phytosterols and maslinic acid extracted from coleus tuberosus in inhibiting the expression of EBV early-antigen in raji cells. Chemistry & Biodiversity, 7 (5). pp. 1267-1275. ISSN 1612-1872, |
| spellingShingle | Q Science (General) Mooi, L.Y. Wahab, N.A. Lajis, N. Ali, A.M. Chemopreventive properties of phytosterols and maslinic acid extracted from coleus tuberosus in inhibiting the expression of EBV early-antigen in raji cells |
| title | Chemopreventive properties of phytosterols and maslinic acid extracted from coleus tuberosus in inhibiting the expression of EBV early-antigen in raji cells |
| title_full | Chemopreventive properties of phytosterols and maslinic acid extracted from coleus tuberosus in inhibiting the expression of EBV early-antigen in raji cells |
| title_fullStr | Chemopreventive properties of phytosterols and maslinic acid extracted from coleus tuberosus in inhibiting the expression of EBV early-antigen in raji cells |
| title_full_unstemmed | Chemopreventive properties of phytosterols and maslinic acid extracted from coleus tuberosus in inhibiting the expression of EBV early-antigen in raji cells |
| title_short | Chemopreventive properties of phytosterols and maslinic acid extracted from coleus tuberosus in inhibiting the expression of EBV early-antigen in raji cells |
| title_sort | chemopreventive properties of phytosterols and maslinic acid extracted from coleus tuberosus in inhibiting the expression of ebv early antigen in raji cells |
| topic | Q Science (General) |
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