Rapid pulsed-field gel electrophoresis method for Streptococcus spp.
Pulsed field gel electrophoresis (PFGE) despite being a highly reproducible and discriminatory method for molecular subtyping of many bacterial pathogens has been criticised for being time consuming. We have developed a modified, rapid PFGE method for Gram positive bacteria, in particular, Streptoco...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society for Molecular Biology and Biotechnology
2001
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| Online Access: | http://eprints.um.edu.my/15365/1/Rapid_PFGE_method_for_strep.Thong_et_al.%2C_2001.pdf |
| _version_ | 1796946893945700352 |
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| author | Thong, Kwai Lin Mohd Nasir, M.D. Goh, Y.L. Ngeow, Y.F. Parasakthi, N. |
| author_facet | Thong, Kwai Lin Mohd Nasir, M.D. Goh, Y.L. Ngeow, Y.F. Parasakthi, N. |
| author_sort | Thong, Kwai Lin |
| collection | UM |
| description | Pulsed field gel electrophoresis (PFGE) despite being a highly reproducible and discriminatory method for molecular subtyping of many bacterial pathogens has been criticised for being time consuming. We have developed a modified, rapid PFGE method for Gram positive bacteria, in particular, Streptococccus spp. Our method can be completed in only 3 days' time as compared to the standard procedure which required Lip to 6 days. We successfully applied this method on clinical isolates of Streptococcus pneumoniae (50) and Group B Streptococcus (50) obtained from the University of Malaya Medical Center with comparable results. The method for DNA preparation was reproducible when repeated analysis was carried out. The rapid method has the ability to process a large number of isolates in less time than the standard method. This will definitely enhance the rapid and accurate analysis of outbreaks of nosocomial or community acquired Streptococcal infections. |
| first_indexed | 2024-03-06T05:38:40Z |
| format | Article |
| id | um.eprints-15365 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:38:40Z |
| publishDate | 2001 |
| publisher | Malaysian Society for Molecular Biology and Biotechnology |
| record_format | dspace |
| spelling | um.eprints-153652021-12-29T05:24:27Z http://eprints.um.edu.my/15365/ Rapid pulsed-field gel electrophoresis method for Streptococcus spp. Thong, Kwai Lin Mohd Nasir, M.D. Goh, Y.L. Ngeow, Y.F. Parasakthi, N. R Medicine Pulsed field gel electrophoresis (PFGE) despite being a highly reproducible and discriminatory method for molecular subtyping of many bacterial pathogens has been criticised for being time consuming. We have developed a modified, rapid PFGE method for Gram positive bacteria, in particular, Streptococccus spp. Our method can be completed in only 3 days' time as compared to the standard procedure which required Lip to 6 days. We successfully applied this method on clinical isolates of Streptococcus pneumoniae (50) and Group B Streptococcus (50) obtained from the University of Malaya Medical Center with comparable results. The method for DNA preparation was reproducible when repeated analysis was carried out. The rapid method has the ability to process a large number of isolates in less time than the standard method. This will definitely enhance the rapid and accurate analysis of outbreaks of nosocomial or community acquired Streptococcal infections. Malaysian Society for Molecular Biology and Biotechnology 2001 Article PeerReviewed text en http://eprints.um.edu.my/15365/1/Rapid_PFGE_method_for_strep.Thong_et_al.%2C_2001.pdf Thong, Kwai Lin and Mohd Nasir, M.D. and Goh, Y.L. and Ngeow, Y.F. and Parasakthi, N. (2001) Rapid pulsed-field gel electrophoresis method for Streptococcus spp. Asia Pacific Journal of Molecular Biology and Biotechnology, 9 (2). pp. 143-145. ISSN 0128-7451, http://www.msmbb.org.my/apjmbb/html92/92143.htm |
| spellingShingle | R Medicine Thong, Kwai Lin Mohd Nasir, M.D. Goh, Y.L. Ngeow, Y.F. Parasakthi, N. Rapid pulsed-field gel electrophoresis method for Streptococcus spp. |
| title | Rapid pulsed-field gel electrophoresis method for Streptococcus spp. |
| title_full | Rapid pulsed-field gel electrophoresis method for Streptococcus spp. |
| title_fullStr | Rapid pulsed-field gel electrophoresis method for Streptococcus spp. |
| title_full_unstemmed | Rapid pulsed-field gel electrophoresis method for Streptococcus spp. |
| title_short | Rapid pulsed-field gel electrophoresis method for Streptococcus spp. |
| title_sort | rapid pulsed field gel electrophoresis method for streptococcus spp |
| topic | R Medicine |
| url | http://eprints.um.edu.my/15365/1/Rapid_PFGE_method_for_strep.Thong_et_al.%2C_2001.pdf |
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