Increasing genetic diversity of Salmonella enterica serovar Typhi isolates from Papua New Guinea over the period from 1992 to 1999
Pulsed-field gel electrophoresis (PFGE) of XbaI-digested chromosomal DNA was performed on 133 strains of Salmonella enterica serovar Typhi obtained from Papua New Guinea, with the objective of assessing the temporal variation of these strains. Fifty-two strains that were isolated in 1992 and 1994 we...
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| Language: | English |
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American Society for Microbiology
2002
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| Online Access: | http://eprints.um.edu.my/15368/1/Increasing_genetic_diversity_of_Salmonella_enterica_serovar_Typhi_isolates_fro_Papua_New_Guinea.pdf |
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| author | Thong, Kwai Lin Goh, Yee Ling Yasin, Rohani M. Lau, Ming Guek Passey, Megan Winston, Gibson Yoannes, Mition Pang, Tikki Reeder, John C. |
| author_facet | Thong, Kwai Lin Goh, Yee Ling Yasin, Rohani M. Lau, Ming Guek Passey, Megan Winston, Gibson Yoannes, Mition Pang, Tikki Reeder, John C. |
| author_sort | Thong, Kwai Lin |
| collection | UM |
| description | Pulsed-field gel electrophoresis (PFGE) of XbaI-digested chromosomal DNA was performed on 133 strains of Salmonella enterica serovar Typhi obtained from Papua New Guinea, with the objective of assessing the temporal variation of these strains. Fifty-two strains that were isolated in 1992 and 1994 were of one phage type, D2, and only two predominant PFGE profiles, X1 and X2, were present. Another 81 strains isolated between 1997 and 1999 have shown divergence, with four new phage types, UVS I (n = 63), UVS (n = 5), VNS (n = 4), and D1 (n = 9), and more genetic variability as evidenced by the multiple and new PFGE XbaI profiles (21 profiles; Dice coefficient, F = 0.71 to 0.97). The two profiles X1 and X2 have remained the stable, dominant subtypes since 1992. Cluster analysis based on the unweighted pair group method using arithmetic averages algorithm identifies two main clusters (at 87% similarity), indicating that the divergence of the PFGE subtypes was probably derived from some genomic mutations of the X1 and X2 subtypes. The majority of isolates were from patients with mild and moderate typhoid fever and had various XbaI profiles. A single isolate from a patient with fatal typhoid fever had a unique X11 profile, while four of six isolates from patients with severe typhoid fever had the X1 pattern. In addition, 12 paired serovar Typhi isolates recovered from the blood and fecal swabs of individual patients exhibited similar PFGE patterns, while in another 11 individuals paired isolates exhibited different PFGE patterns. Three pairs of isolates recovered from three individuals had different phage types and PFGE patterns, indicating infection with multiple strains. The study reiterates the usefulness of PFGE in assessing the genetic diversity of S. enterica serovar Typhi for both long-term epidemiology and in vivo stability and instability within an individual patient.
Typhoid fever is a systemic prolonged febrile illness caused by Salmonella enterica serovar Typhi. It continues to be a worldwide health problem, especially in developing countries with their poor sanitation, poor standards of personal hygiene, and contaminated food. Current estimates from the World Health Organization suggest that the worldwide incidence of typhoid fever is approximately 16 million cases annually, with more than 600,000 deaths. Effective epidemiological surveillance is needed to monitor the presence and spread of the disease. For serovar Typhi, the primary tools are serotyping and phage typing (6). However, these phenotypic methods lack discrimination and are often complemented by the more sensitive and discriminative molecular techniques (1). Pulsed-field gel electrophoresis (PFGE) is one of the most common technique used to perform comparative chromosomal DNA analysis of serovar Typhi (6, 15, 21).
Previous studies of serovar Typhi have shown significant genetic diversity, as demonstrated by PFGE differences among 400 isolates obtained from various regions with endemic infection, including Malaysia, Indonesia, Thailand, and Chile (21, 22, 23). In contrast, PFGE analysis of a collection of 52 serovar Typhi isolates obtained in 1992 to 1994 from Papua New Guinea were very homogeneous (25). The present study was carried out to extend the monitoring of genetic diversity to more recent isolations of serovar Typhi strains from Papua New Guinea with respect to the phage types and PFGE profiles. |
| first_indexed | 2024-03-06T05:38:40Z |
| format | Article |
| id | um.eprints-15368 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:38:40Z |
| publishDate | 2002 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | um.eprints-153682021-12-30T09:03:33Z http://eprints.um.edu.my/15368/ Increasing genetic diversity of Salmonella enterica serovar Typhi isolates from Papua New Guinea over the period from 1992 to 1999 Thong, Kwai Lin Goh, Yee Ling Yasin, Rohani M. Lau, Ming Guek Passey, Megan Winston, Gibson Yoannes, Mition Pang, Tikki Reeder, John C. Q Science (General) QH Natural history QR Microbiology Pulsed-field gel electrophoresis (PFGE) of XbaI-digested chromosomal DNA was performed on 133 strains of Salmonella enterica serovar Typhi obtained from Papua New Guinea, with the objective of assessing the temporal variation of these strains. Fifty-two strains that were isolated in 1992 and 1994 were of one phage type, D2, and only two predominant PFGE profiles, X1 and X2, were present. Another 81 strains isolated between 1997 and 1999 have shown divergence, with four new phage types, UVS I (n = 63), UVS (n = 5), VNS (n = 4), and D1 (n = 9), and more genetic variability as evidenced by the multiple and new PFGE XbaI profiles (21 profiles; Dice coefficient, F = 0.71 to 0.97). The two profiles X1 and X2 have remained the stable, dominant subtypes since 1992. Cluster analysis based on the unweighted pair group method using arithmetic averages algorithm identifies two main clusters (at 87% similarity), indicating that the divergence of the PFGE subtypes was probably derived from some genomic mutations of the X1 and X2 subtypes. The majority of isolates were from patients with mild and moderate typhoid fever and had various XbaI profiles. A single isolate from a patient with fatal typhoid fever had a unique X11 profile, while four of six isolates from patients with severe typhoid fever had the X1 pattern. In addition, 12 paired serovar Typhi isolates recovered from the blood and fecal swabs of individual patients exhibited similar PFGE patterns, while in another 11 individuals paired isolates exhibited different PFGE patterns. Three pairs of isolates recovered from three individuals had different phage types and PFGE patterns, indicating infection with multiple strains. The study reiterates the usefulness of PFGE in assessing the genetic diversity of S. enterica serovar Typhi for both long-term epidemiology and in vivo stability and instability within an individual patient. Typhoid fever is a systemic prolonged febrile illness caused by Salmonella enterica serovar Typhi. It continues to be a worldwide health problem, especially in developing countries with their poor sanitation, poor standards of personal hygiene, and contaminated food. Current estimates from the World Health Organization suggest that the worldwide incidence of typhoid fever is approximately 16 million cases annually, with more than 600,000 deaths. Effective epidemiological surveillance is needed to monitor the presence and spread of the disease. For serovar Typhi, the primary tools are serotyping and phage typing (6). However, these phenotypic methods lack discrimination and are often complemented by the more sensitive and discriminative molecular techniques (1). Pulsed-field gel electrophoresis (PFGE) is one of the most common technique used to perform comparative chromosomal DNA analysis of serovar Typhi (6, 15, 21). Previous studies of serovar Typhi have shown significant genetic diversity, as demonstrated by PFGE differences among 400 isolates obtained from various regions with endemic infection, including Malaysia, Indonesia, Thailand, and Chile (21, 22, 23). In contrast, PFGE analysis of a collection of 52 serovar Typhi isolates obtained in 1992 to 1994 from Papua New Guinea were very homogeneous (25). The present study was carried out to extend the monitoring of genetic diversity to more recent isolations of serovar Typhi strains from Papua New Guinea with respect to the phage types and PFGE profiles. American Society for Microbiology 2002 Article PeerReviewed application/pdf en http://eprints.um.edu.my/15368/1/Increasing_genetic_diversity_of_Salmonella_enterica_serovar_Typhi_isolates_fro_Papua_New_Guinea.pdf Thong, Kwai Lin and Goh, Yee Ling and Yasin, Rohani M. and Lau, Ming Guek and Passey, Megan and Winston, Gibson and Yoannes, Mition and Pang, Tikki and Reeder, John C. (2002) Increasing genetic diversity of Salmonella enterica serovar Typhi isolates from Papua New Guinea over the period from 1992 to 1999. Journal of Clinical Microbiology, 40 (11). pp. 4156-4160. ISSN 0095-1137, DOI https://doi.org/10.1128/JCM.40.11.4156-4160.2002 <https://doi.org/10.1128/JCM.40.11.4156-4160.2002>. https://doi.org/10.1128/JCM.40.11.4156-4160.2002 doi:10.1128/JCM.40.11.4156-4160.2002 |
| spellingShingle | Q Science (General) QH Natural history QR Microbiology Thong, Kwai Lin Goh, Yee Ling Yasin, Rohani M. Lau, Ming Guek Passey, Megan Winston, Gibson Yoannes, Mition Pang, Tikki Reeder, John C. Increasing genetic diversity of Salmonella enterica serovar Typhi isolates from Papua New Guinea over the period from 1992 to 1999 |
| title | Increasing genetic diversity of Salmonella enterica serovar Typhi isolates from Papua New Guinea over the period from 1992 to 1999 |
| title_full | Increasing genetic diversity of Salmonella enterica serovar Typhi isolates from Papua New Guinea over the period from 1992 to 1999 |
| title_fullStr | Increasing genetic diversity of Salmonella enterica serovar Typhi isolates from Papua New Guinea over the period from 1992 to 1999 |
| title_full_unstemmed | Increasing genetic diversity of Salmonella enterica serovar Typhi isolates from Papua New Guinea over the period from 1992 to 1999 |
| title_short | Increasing genetic diversity of Salmonella enterica serovar Typhi isolates from Papua New Guinea over the period from 1992 to 1999 |
| title_sort | increasing genetic diversity of salmonella enterica serovar typhi isolates from papua new guinea over the period from 1992 to 1999 |
| topic | Q Science (General) QH Natural history QR Microbiology |
| url | http://eprints.um.edu.my/15368/1/Increasing_genetic_diversity_of_Salmonella_enterica_serovar_Typhi_isolates_fro_Papua_New_Guinea.pdf |
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