Polymethacrylate coated electrospun PHB fibers: An exquisite outlook for fabrication of paper-based biosensors
Electrospun polyhydroxybutyrate (PHB) fibers were dip-coated by polymethyl methacrylate-co-methacrylic acid, poly(MMA-co-MAA), which was synthesized in different molar ratios of the monomers via free-radical polymerization. Fabricated platfrom was employed for immobilization of the dengue antibody a...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015
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| Online Access: | http://eprints.um.edu.my/15688/1/Polymethacrylate_coated_electrospun_PHB_fibers_An_exquisite_outlook_for_fabrication.pdf |
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| author | Hosseini, S. Azari, P. Farahmand, E. Gan, S.N. Rothan, H.A. Yusof, R. Koole, L.H. Djordjevic, I. Ibrahim, F. |
| author_facet | Hosseini, S. Azari, P. Farahmand, E. Gan, S.N. Rothan, H.A. Yusof, R. Koole, L.H. Djordjevic, I. Ibrahim, F. |
| author_sort | Hosseini, S. |
| collection | UM |
| description | Electrospun polyhydroxybutyrate (PHB) fibers were dip-coated by polymethyl methacrylate-co-methacrylic acid, poly(MMA-co-MAA), which was synthesized in different molar ratios of the monomers via free-radical polymerization. Fabricated platfrom was employed for immobilization of the dengue antibody and subsequent detection of dengue enveloped virus in enzyme-linked immunosorbent assay (ELISA). There is a major advantage for combination of electrospun fibers and copolymers. Fiber structre of electrospun PHB provides large specific surface area available for biomolecular interaction. In addition, polymer coated parts of the platform inherited the premanent presence of surface carboxyl (-COOH) groups from MAA segments of the copolymer which can be effectively used for covalent and physical protein immobilization. By tuning the concentration of MAA monomers in polymerization reaction the concentration of surface -COOH groups can be carefully controlled. Therefore two different techniques have been used for immobilization of the dengue antibody aimed for dengue detection: physical attachment of dengue antibodies to the surface and covalent immobilization of antibodies through carbodiimide chemistry. In that perspective, several different characterization techniques were employed to investigate the new polymeric fiber platform such as scanning electron microscopy (SEM), atomic force microscopy (AFM), water contact angle (WCA) measurement and UV-vis titration. Regardless of the immobilization techniques, substantially higher signal intensity was recorded from developed platform in comparison to the conventional ELISA assay. (C) 2015 Elsevier B.V. All rights reserved. |
| first_indexed | 2024-03-06T05:39:23Z |
| format | Article |
| id | um.eprints-15688 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:39:23Z |
| publishDate | 2015 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | um.eprints-156882017-11-01T06:07:32Z http://eprints.um.edu.my/15688/ Polymethacrylate coated electrospun PHB fibers: An exquisite outlook for fabrication of paper-based biosensors Hosseini, S. Azari, P. Farahmand, E. Gan, S.N. Rothan, H.A. Yusof, R. Koole, L.H. Djordjevic, I. Ibrahim, F. T Technology (General) TA Engineering (General). Civil engineering (General) Electrospun polyhydroxybutyrate (PHB) fibers were dip-coated by polymethyl methacrylate-co-methacrylic acid, poly(MMA-co-MAA), which was synthesized in different molar ratios of the monomers via free-radical polymerization. Fabricated platfrom was employed for immobilization of the dengue antibody and subsequent detection of dengue enveloped virus in enzyme-linked immunosorbent assay (ELISA). There is a major advantage for combination of electrospun fibers and copolymers. Fiber structre of electrospun PHB provides large specific surface area available for biomolecular interaction. In addition, polymer coated parts of the platform inherited the premanent presence of surface carboxyl (-COOH) groups from MAA segments of the copolymer which can be effectively used for covalent and physical protein immobilization. By tuning the concentration of MAA monomers in polymerization reaction the concentration of surface -COOH groups can be carefully controlled. Therefore two different techniques have been used for immobilization of the dengue antibody aimed for dengue detection: physical attachment of dengue antibodies to the surface and covalent immobilization of antibodies through carbodiimide chemistry. In that perspective, several different characterization techniques were employed to investigate the new polymeric fiber platform such as scanning electron microscopy (SEM), atomic force microscopy (AFM), water contact angle (WCA) measurement and UV-vis titration. Regardless of the immobilization techniques, substantially higher signal intensity was recorded from developed platform in comparison to the conventional ELISA assay. (C) 2015 Elsevier B.V. All rights reserved. Elsevier 2015-07-15 Article PeerReviewed application/pdf en http://eprints.um.edu.my/15688/1/Polymethacrylate_coated_electrospun_PHB_fibers_An_exquisite_outlook_for_fabrication.pdf Hosseini, S. and Azari, P. and Farahmand, E. and Gan, S.N. and Rothan, H.A. and Yusof, R. and Koole, L.H. and Djordjevic, I. and Ibrahim, F. (2015) Polymethacrylate coated electrospun PHB fibers: An exquisite outlook for fabrication of paper-based biosensors. Biosensors and Bioelectronics, 69. pp. 257-264. ISSN 0956-5663, DOI https://doi.org/10.1016/j.bios.2015.02.034 <https://doi.org/10.1016/j.bios.2015.02.034>. http://www.sciencedirect.com/science/article/pii/S0956566315001359 10.1016/j.bios.2015.02.034 |
| spellingShingle | T Technology (General) TA Engineering (General). Civil engineering (General) Hosseini, S. Azari, P. Farahmand, E. Gan, S.N. Rothan, H.A. Yusof, R. Koole, L.H. Djordjevic, I. Ibrahim, F. Polymethacrylate coated electrospun PHB fibers: An exquisite outlook for fabrication of paper-based biosensors |
| title | Polymethacrylate coated electrospun PHB fibers: An exquisite outlook for fabrication of paper-based biosensors |
| title_full | Polymethacrylate coated electrospun PHB fibers: An exquisite outlook for fabrication of paper-based biosensors |
| title_fullStr | Polymethacrylate coated electrospun PHB fibers: An exquisite outlook for fabrication of paper-based biosensors |
| title_full_unstemmed | Polymethacrylate coated electrospun PHB fibers: An exquisite outlook for fabrication of paper-based biosensors |
| title_short | Polymethacrylate coated electrospun PHB fibers: An exquisite outlook for fabrication of paper-based biosensors |
| title_sort | polymethacrylate coated electrospun phb fibers an exquisite outlook for fabrication of paper based biosensors |
| topic | T Technology (General) TA Engineering (General). Civil engineering (General) |
| url | http://eprints.um.edu.my/15688/1/Polymethacrylate_coated_electrospun_PHB_fibers_An_exquisite_outlook_for_fabrication.pdf |
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