Immunodominant IgM and IgG epitopes recognized by antibodies induced in enterovirus A71-associated hand, foot and mouth disease patients
Enterovirus A71 (EV-A71) is one of the main causative agents of hand, foot and mouth disease (HFMD). Unlike other enteroviruses that cause HFMD, EV-A71 is more frequently associated with severe neurological complications and fatality. Little is known about the immunogenicity of viral non-structural...
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| Format: | Conference or Workshop Item |
| Language: | English |
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2016
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| Online Access: | http://eprints.um.edu.my/16614/1/abstract_yoke_fun_chan.pdf |
| _version_ | 1825720858745765888 |
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| author | Chan, Y.F. |
| author_facet | Chan, Y.F. |
| author_sort | Chan, Y.F. |
| collection | UM |
| description | Enterovirus A71 (EV-A71) is one of the main causative agents of hand, foot and mouth disease (HFMD). Unlike other enteroviruses that cause HFMD, EV-A71 is more frequently associated with severe neurological complications and fatality. Little is known about the immunogenicity of viral non-structural proteins in humans. Previous studies have mainly focused on characterization of epitopes of EV-A71 structural proteins by using immunized animal antisera. In this study, we have characterized human antibody responses against the structural and non-structural proteins of EV-A71. Each viral protein was cloned and expressed in either bacterial or mammalian systems, and tested with antisera by western blot. Results revealed that all structural proteins (VP1-4), and non-structural proteins 2A, 3C and 3D were targets of EV-A71 IgM, whereas EV-A71 IgG recognized all the structural and non-structural proteins. Sixty three synthetic peptides predicted to be immunogenic in silico were synthesized and used for the characterization of EV-A71 linear B-cell epitopes. In total, we identified 22 IgM and 4 IgG dominant epitopes. Synthetic peptide PEP27, corresponding to residues 142-156 of VP1, was identified as the EV-A71 IgM-specific immunodominant epitope; and PEP23 mapped at VP1 41-55 was recognized as the EV-A71 IgG cross-reactive immunodominant epitope. The structural protein VP1 is the major immunodominant site targeted by anti-EV-A71 IgM and IgG antibodies. These data provide new understanding of the immune response to EV-A71 infection, which benefits the development of diagnostic tools, potential therapeutics and subunit vaccine candidates. |
| first_indexed | 2024-03-06T05:41:30Z |
| format | Conference or Workshop Item |
| id | um.eprints-16614 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:41:30Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | um.eprints-166142016-11-02T07:06:06Z http://eprints.um.edu.my/16614/ Immunodominant IgM and IgG epitopes recognized by antibodies induced in enterovirus A71-associated hand, foot and mouth disease patients Chan, Y.F. R Medicine (General) Enterovirus A71 (EV-A71) is one of the main causative agents of hand, foot and mouth disease (HFMD). Unlike other enteroviruses that cause HFMD, EV-A71 is more frequently associated with severe neurological complications and fatality. Little is known about the immunogenicity of viral non-structural proteins in humans. Previous studies have mainly focused on characterization of epitopes of EV-A71 structural proteins by using immunized animal antisera. In this study, we have characterized human antibody responses against the structural and non-structural proteins of EV-A71. Each viral protein was cloned and expressed in either bacterial or mammalian systems, and tested with antisera by western blot. Results revealed that all structural proteins (VP1-4), and non-structural proteins 2A, 3C and 3D were targets of EV-A71 IgM, whereas EV-A71 IgG recognized all the structural and non-structural proteins. Sixty three synthetic peptides predicted to be immunogenic in silico were synthesized and used for the characterization of EV-A71 linear B-cell epitopes. In total, we identified 22 IgM and 4 IgG dominant epitopes. Synthetic peptide PEP27, corresponding to residues 142-156 of VP1, was identified as the EV-A71 IgM-specific immunodominant epitope; and PEP23 mapped at VP1 41-55 was recognized as the EV-A71 IgG cross-reactive immunodominant epitope. The structural protein VP1 is the major immunodominant site targeted by anti-EV-A71 IgM and IgG antibodies. These data provide new understanding of the immune response to EV-A71 infection, which benefits the development of diagnostic tools, potential therapeutics and subunit vaccine candidates. 2016 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.um.edu.my/16614/1/abstract_yoke_fun_chan.pdf Chan, Y.F. (2016) Immunodominant IgM and IgG epitopes recognized by antibodies induced in enterovirus A71-associated hand, foot and mouth disease patients. In: European Study Group on the Molecular Biology of Picornaviruses Conference 2016, 04 - 08 September 2016, Les Diablerets, Switzerland. |
| spellingShingle | R Medicine (General) Chan, Y.F. Immunodominant IgM and IgG epitopes recognized by antibodies induced in enterovirus A71-associated hand, foot and mouth disease patients |
| title | Immunodominant IgM and IgG epitopes recognized by antibodies induced in enterovirus A71-associated hand, foot and mouth disease patients |
| title_full | Immunodominant IgM and IgG epitopes recognized by antibodies induced in enterovirus A71-associated hand, foot and mouth disease patients |
| title_fullStr | Immunodominant IgM and IgG epitopes recognized by antibodies induced in enterovirus A71-associated hand, foot and mouth disease patients |
| title_full_unstemmed | Immunodominant IgM and IgG epitopes recognized by antibodies induced in enterovirus A71-associated hand, foot and mouth disease patients |
| title_short | Immunodominant IgM and IgG epitopes recognized by antibodies induced in enterovirus A71-associated hand, foot and mouth disease patients |
| title_sort | immunodominant igm and igg epitopes recognized by antibodies induced in enterovirus a71 associated hand foot and mouth disease patients |
| topic | R Medicine (General) |
| url | http://eprints.um.edu.my/16614/1/abstract_yoke_fun_chan.pdf |
| work_keys_str_mv | AT chanyf immunodominantigmandiggepitopesrecognizedbyantibodiesinducedinenterovirusa71associatedhandfootandmouthdiseasepatients |