Polymerase Chain Reaction (PCR) Techniques For The Identification Of Uncultivable Oral Microorganisms
Until today there are still a high percentage of oral microorganisms have not been identified due to inability to isolate using the cultural method. However, identification of uncultivable microorganisms associated with disease will permits clinicians for a more accurate diagnosis, treatment and pre...
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| Format: | Article |
| Language: | English |
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Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia
2001
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| Online Access: | http://eprints.um.edu.my/17392/1/Himratul-Anita_W.H..pdf |
| _version_ | 1796947140263542784 |
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| author | Himratul-Aznita, W.H. |
| author_facet | Himratul-Aznita, W.H. |
| author_sort | Himratul-Aznita, W.H. |
| collection | UM |
| description | Until today there are still a high percentage of oral microorganisms have not been identified due to inability to isolate using the cultural method. However, identification of uncultivable microorganisms associated with disease will permits clinicians for a more accurate diagnosis, treatment and preventive measures. Unculturable microorganisms are also involved in disease and may account for treatment failure since their susceptibility to antimicrobial agents would be unknown. Thus, the opportunity for a rational approach to the treatment of disease relies on the state of knowledge concerning its aetiology and pathogenesis. Recently developed molecular methods have made it possible to characterise mixed microflora in their entirety, including the substantial numbers of unculturable bacteria. The development of rapid molecular methods like PCR provides a reliable identification of unculturable microorganisms. This paper will review the current literature regarding the PCR techniques used to identify uncultivable oral microflora. |
| first_indexed | 2024-03-06T05:42:24Z |
| format | Article |
| id | um.eprints-17392 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:42:24Z |
| publishDate | 2001 |
| publisher | Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia |
| record_format | dspace |
| spelling | um.eprints-173922017-05-30T12:45:03Z http://eprints.um.edu.my/17392/ Polymerase Chain Reaction (PCR) Techniques For The Identification Of Uncultivable Oral Microorganisms Himratul-Aznita, W.H. RK Dentistry Until today there are still a high percentage of oral microorganisms have not been identified due to inability to isolate using the cultural method. However, identification of uncultivable microorganisms associated with disease will permits clinicians for a more accurate diagnosis, treatment and preventive measures. Unculturable microorganisms are also involved in disease and may account for treatment failure since their susceptibility to antimicrobial agents would be unknown. Thus, the opportunity for a rational approach to the treatment of disease relies on the state of knowledge concerning its aetiology and pathogenesis. Recently developed molecular methods have made it possible to characterise mixed microflora in their entirety, including the substantial numbers of unculturable bacteria. The development of rapid molecular methods like PCR provides a reliable identification of unculturable microorganisms. This paper will review the current literature regarding the PCR techniques used to identify uncultivable oral microflora. Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia 2001 Article PeerReviewed application/pdf en http://eprints.um.edu.my/17392/1/Himratul-Anita_W.H..pdf Himratul-Aznita, W.H. (2001) Polymerase Chain Reaction (PCR) Techniques For The Identification Of Uncultivable Oral Microorganisms. Annals of Dentistry, 8 (1). pp. 43-49. ISSN 0128-7532, http://ejum.fsktm.um.edu.my/article/428.pdf |
| spellingShingle | RK Dentistry Himratul-Aznita, W.H. Polymerase Chain Reaction (PCR) Techniques For The Identification Of Uncultivable Oral Microorganisms |
| title | Polymerase Chain Reaction (PCR) Techniques For The Identification Of Uncultivable Oral Microorganisms |
| title_full | Polymerase Chain Reaction (PCR) Techniques For The Identification Of Uncultivable Oral Microorganisms |
| title_fullStr | Polymerase Chain Reaction (PCR) Techniques For The Identification Of Uncultivable Oral Microorganisms |
| title_full_unstemmed | Polymerase Chain Reaction (PCR) Techniques For The Identification Of Uncultivable Oral Microorganisms |
| title_short | Polymerase Chain Reaction (PCR) Techniques For The Identification Of Uncultivable Oral Microorganisms |
| title_sort | polymerase chain reaction pcr techniques for the identification of uncultivable oral microorganisms |
| topic | RK Dentistry |
| url | http://eprints.um.edu.my/17392/1/Himratul-Anita_W.H..pdf |
| work_keys_str_mv | AT himratulaznitawh polymerasechainreactionpcrtechniquesfortheidentificationofuncultivableoralmicroorganisms |