Subversion of immunoproteasome subunit expression in dengue virus serotype 2-infected HepG2 cells
Introduction: Infection with all serotypes of dengue virus (DV) results in augmented antigen presentation by MHC class I molecules. However, the upregulation of immunoproteasome subunits only results from infection with two serotypes. This study aims to elucidate changes in the expression of immunop...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Medicina Tropical (SBMT)
2017
|
| Subjects: | |
| Online Access: | http://eprints.um.edu.my/17649/1/Gan%2C_CS_%282017%29.pdf |
| _version_ | 1796947188413104128 |
|---|---|
| author | Gan, C.S. Lim, P.J. Razif, M.F.M. Yusof, R. Othman, S. |
| author_facet | Gan, C.S. Lim, P.J. Razif, M.F.M. Yusof, R. Othman, S. |
| author_sort | Gan, C.S. |
| collection | UM |
| description | Introduction: Infection with all serotypes of dengue virus (DV) results in augmented antigen presentation by MHC class I molecules. However, the upregulation of immunoproteasome subunits only results from infection with two serotypes. This study aims to elucidate changes in the expression of immunoproteasome subunits resulting from infection with DV, particularly DV serotype 2 (DV2). Methods: HepG2 cells were grown in various culture milieu. Total cellular RNA and proteins were extracted and quantified. Results: Results demonstrated sequestration of immunoproteasome subunits LMP2 and LMP7 in DV2-infected cells. Conclusions: This study provides insights into the mechanisms underlying immune evasion by DV. |
| first_indexed | 2024-03-06T05:43:08Z |
| format | Article |
| id | um.eprints-17649 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:43:08Z |
| publishDate | 2017 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | dspace |
| spelling | um.eprints-176492017-08-07T07:11:20Z http://eprints.um.edu.my/17649/ Subversion of immunoproteasome subunit expression in dengue virus serotype 2-infected HepG2 cells Gan, C.S. Lim, P.J. Razif, M.F.M. Yusof, R. Othman, S. R Medicine Introduction: Infection with all serotypes of dengue virus (DV) results in augmented antigen presentation by MHC class I molecules. However, the upregulation of immunoproteasome subunits only results from infection with two serotypes. This study aims to elucidate changes in the expression of immunoproteasome subunits resulting from infection with DV, particularly DV serotype 2 (DV2). Methods: HepG2 cells were grown in various culture milieu. Total cellular RNA and proteins were extracted and quantified. Results: Results demonstrated sequestration of immunoproteasome subunits LMP2 and LMP7 in DV2-infected cells. Conclusions: This study provides insights into the mechanisms underlying immune evasion by DV. Sociedade Brasileira de Medicina Tropical (SBMT) 2017 Article PeerReviewed application/pdf en http://eprints.um.edu.my/17649/1/Gan%2C_CS_%282017%29.pdf Gan, C.S. and Lim, P.J. and Razif, M.F.M. and Yusof, R. and Othman, S. (2017) Subversion of immunoproteasome subunit expression in dengue virus serotype 2-infected HepG2 cells. Revista da Sociedade Brasileira de Medicina Tropical, 50 (1). pp. 99-103. ISSN 0037-8682, DOI https://doi.org/10.1590/0037-8682-0207-2016 <https://doi.org/10.1590/0037-8682-0207-2016>. http://dx.doi.org/10.1590/0037-8682-0207-2016 doi:10.1590/0037-8682-0207-2016 |
| spellingShingle | R Medicine Gan, C.S. Lim, P.J. Razif, M.F.M. Yusof, R. Othman, S. Subversion of immunoproteasome subunit expression in dengue virus serotype 2-infected HepG2 cells |
| title | Subversion of immunoproteasome subunit expression in dengue virus serotype 2-infected HepG2 cells |
| title_full | Subversion of immunoproteasome subunit expression in dengue virus serotype 2-infected HepG2 cells |
| title_fullStr | Subversion of immunoproteasome subunit expression in dengue virus serotype 2-infected HepG2 cells |
| title_full_unstemmed | Subversion of immunoproteasome subunit expression in dengue virus serotype 2-infected HepG2 cells |
| title_short | Subversion of immunoproteasome subunit expression in dengue virus serotype 2-infected HepG2 cells |
| title_sort | subversion of immunoproteasome subunit expression in dengue virus serotype 2 infected hepg2 cells |
| topic | R Medicine |
| url | http://eprints.um.edu.my/17649/1/Gan%2C_CS_%282017%29.pdf |
| work_keys_str_mv | AT gancs subversionofimmunoproteasomesubunitexpressionindenguevirusserotype2infectedhepg2cells AT limpj subversionofimmunoproteasomesubunitexpressionindenguevirusserotype2infectedhepg2cells AT razifmfm subversionofimmunoproteasomesubunitexpressionindenguevirusserotype2infectedhepg2cells AT yusofr subversionofimmunoproteasomesubunitexpressionindenguevirusserotype2infectedhepg2cells AT othmans subversionofimmunoproteasomesubunitexpressionindenguevirusserotype2infectedhepg2cells |