Anticancer activity of a monobenzyltin complex C1 against MDA-MB-231 cells through induction of Apoptosis and inhibition of breast cancer stem cells
In the present study, we examined the cytotoxic effects of Schiff base complex, [N-(3,5-dichloro-2-oxidobenzylidene)-4-chlorobenzyhydrazidato](o-methylbenzyl)aquatin(IV) chloride, and C1 on MDA-MB-231 cells and derived breast cancer stem cells from MDA-MB-231 cells. The acute toxicity experiment wit...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2016
|
| Subjects: | |
| Online Access: | http://eprints.um.edu.my/17694/1/Fani%2C_S._%282016%29.pdf |
| _version_ | 1825720972935692288 |
|---|---|
| author | Fani, S. Kamalidehghan, B. Lo, Kong Mun Nigjeh, S.E. Keong, Y.S. Dehghan, F. Soori, R. Abdulla, Mahmood Ameen Chow, K.M. Ali, Hapipah Mohd Hajiaghaalipour, F. Rouhollahi, E. Hashim, N.M. |
| author_facet | Fani, S. Kamalidehghan, B. Lo, Kong Mun Nigjeh, S.E. Keong, Y.S. Dehghan, F. Soori, R. Abdulla, Mahmood Ameen Chow, K.M. Ali, Hapipah Mohd Hajiaghaalipour, F. Rouhollahi, E. Hashim, N.M. |
| author_sort | Fani, S. |
| collection | UM |
| description | In the present study, we examined the cytotoxic effects of Schiff base complex, [N-(3,5-dichloro-2-oxidobenzylidene)-4-chlorobenzyhydrazidato](o-methylbenzyl)aquatin(IV) chloride, and C1 on MDA-MB-231 cells and derived breast cancer stem cells from MDA-MB-231 cells. The acute toxicity experiment with compound C1 revealed no cytotoxic effects on rats. Fluorescent microscopic studies using Acridine Orange/Propidium Iodide (AO/PI) staining and flow cytometric analysis using an Annexin V probe confirmed the occurrence of apoptosis in C1-treated MDA-MB-231 cells. Compound C1 triggered intracellular reactive oxygen species (ROS) production and lactate dehydrogenase (LDH) releases in treated MDA-MB-231 cells. The Cellomics High Content Screening (HCS) analysis showed the induction of intrinsic pathways in treated MDA-MB-231 cells, and a luminescence assay revealed significant increases in caspase 9 and 3/7 activity. Furthermore, flow cytometric analysis showed that compound C1 induced G0/G1 arrest in treated MDA-MB-231 cells. Real time PCR and western blot analysis revealed the upregulation of the Bax protein and the downregulation of the Bcl-2 and HSP70 proteins. Additionally, this study revealed the suppressive effect of compound C1 against breast CSCs and its ability to inhibit the Wnt/β-catenin signaling pathways. Our results demonstrate the chemotherapeutic properties of compound C1 against breast cancer cells and derived breast cancer stem cells, suggesting that the anticancer capabilities of this compound should be clinically assessed. |
| first_indexed | 2024-03-06T05:43:16Z |
| format | Article |
| id | um.eprints-17694 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:43:16Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | um.eprints-176942019-01-30T01:46:08Z http://eprints.um.edu.my/17694/ Anticancer activity of a monobenzyltin complex C1 against MDA-MB-231 cells through induction of Apoptosis and inhibition of breast cancer stem cells Fani, S. Kamalidehghan, B. Lo, Kong Mun Nigjeh, S.E. Keong, Y.S. Dehghan, F. Soori, R. Abdulla, Mahmood Ameen Chow, K.M. Ali, Hapipah Mohd Hajiaghaalipour, F. Rouhollahi, E. Hashim, N.M. Q Science (General) R Medicine In the present study, we examined the cytotoxic effects of Schiff base complex, [N-(3,5-dichloro-2-oxidobenzylidene)-4-chlorobenzyhydrazidato](o-methylbenzyl)aquatin(IV) chloride, and C1 on MDA-MB-231 cells and derived breast cancer stem cells from MDA-MB-231 cells. The acute toxicity experiment with compound C1 revealed no cytotoxic effects on rats. Fluorescent microscopic studies using Acridine Orange/Propidium Iodide (AO/PI) staining and flow cytometric analysis using an Annexin V probe confirmed the occurrence of apoptosis in C1-treated MDA-MB-231 cells. Compound C1 triggered intracellular reactive oxygen species (ROS) production and lactate dehydrogenase (LDH) releases in treated MDA-MB-231 cells. The Cellomics High Content Screening (HCS) analysis showed the induction of intrinsic pathways in treated MDA-MB-231 cells, and a luminescence assay revealed significant increases in caspase 9 and 3/7 activity. Furthermore, flow cytometric analysis showed that compound C1 induced G0/G1 arrest in treated MDA-MB-231 cells. Real time PCR and western blot analysis revealed the upregulation of the Bax protein and the downregulation of the Bcl-2 and HSP70 proteins. Additionally, this study revealed the suppressive effect of compound C1 against breast CSCs and its ability to inhibit the Wnt/β-catenin signaling pathways. Our results demonstrate the chemotherapeutic properties of compound C1 against breast cancer cells and derived breast cancer stem cells, suggesting that the anticancer capabilities of this compound should be clinically assessed. Nature Publishing Group 2016 Article PeerReviewed application/pdf en http://eprints.um.edu.my/17694/1/Fani%2C_S._%282016%29.pdf Fani, S. and Kamalidehghan, B. and Lo, Kong Mun and Nigjeh, S.E. and Keong, Y.S. and Dehghan, F. and Soori, R. and Abdulla, Mahmood Ameen and Chow, K.M. and Ali, Hapipah Mohd and Hajiaghaalipour, F. and Rouhollahi, E. and Hashim, N.M. (2016) Anticancer activity of a monobenzyltin complex C1 against MDA-MB-231 cells through induction of Apoptosis and inhibition of breast cancer stem cells. Scientific Reports, 6. p. 38992. ISSN 2045-2322, DOI https://doi.org/10.1038/srep38992 <https://doi.org/10.1038/srep38992>. http://dx.doi.org/10.1038/srep38992 doi:10.1038/srep38992 |
| spellingShingle | Q Science (General) R Medicine Fani, S. Kamalidehghan, B. Lo, Kong Mun Nigjeh, S.E. Keong, Y.S. Dehghan, F. Soori, R. Abdulla, Mahmood Ameen Chow, K.M. Ali, Hapipah Mohd Hajiaghaalipour, F. Rouhollahi, E. Hashim, N.M. Anticancer activity of a monobenzyltin complex C1 against MDA-MB-231 cells through induction of Apoptosis and inhibition of breast cancer stem cells |
| title | Anticancer activity of a monobenzyltin complex C1 against MDA-MB-231 cells through induction of Apoptosis and inhibition of breast cancer stem cells |
| title_full | Anticancer activity of a monobenzyltin complex C1 against MDA-MB-231 cells through induction of Apoptosis and inhibition of breast cancer stem cells |
| title_fullStr | Anticancer activity of a monobenzyltin complex C1 against MDA-MB-231 cells through induction of Apoptosis and inhibition of breast cancer stem cells |
| title_full_unstemmed | Anticancer activity of a monobenzyltin complex C1 against MDA-MB-231 cells through induction of Apoptosis and inhibition of breast cancer stem cells |
| title_short | Anticancer activity of a monobenzyltin complex C1 against MDA-MB-231 cells through induction of Apoptosis and inhibition of breast cancer stem cells |
| title_sort | anticancer activity of a monobenzyltin complex c1 against mda mb 231 cells through induction of apoptosis and inhibition of breast cancer stem cells |
| topic | Q Science (General) R Medicine |
| url | http://eprints.um.edu.my/17694/1/Fani%2C_S._%282016%29.pdf |
| work_keys_str_mv | AT fanis anticanceractivityofamonobenzyltincomplexc1againstmdamb231cellsthroughinductionofapoptosisandinhibitionofbreastcancerstemcells AT kamalidehghanb anticanceractivityofamonobenzyltincomplexc1againstmdamb231cellsthroughinductionofapoptosisandinhibitionofbreastcancerstemcells AT lokongmun anticanceractivityofamonobenzyltincomplexc1againstmdamb231cellsthroughinductionofapoptosisandinhibitionofbreastcancerstemcells AT nigjehse anticanceractivityofamonobenzyltincomplexc1againstmdamb231cellsthroughinductionofapoptosisandinhibitionofbreastcancerstemcells AT keongys anticanceractivityofamonobenzyltincomplexc1againstmdamb231cellsthroughinductionofapoptosisandinhibitionofbreastcancerstemcells AT dehghanf anticanceractivityofamonobenzyltincomplexc1againstmdamb231cellsthroughinductionofapoptosisandinhibitionofbreastcancerstemcells AT soorir anticanceractivityofamonobenzyltincomplexc1againstmdamb231cellsthroughinductionofapoptosisandinhibitionofbreastcancerstemcells AT abdullamahmoodameen anticanceractivityofamonobenzyltincomplexc1againstmdamb231cellsthroughinductionofapoptosisandinhibitionofbreastcancerstemcells AT chowkm anticanceractivityofamonobenzyltincomplexc1againstmdamb231cellsthroughinductionofapoptosisandinhibitionofbreastcancerstemcells AT alihapipahmohd anticanceractivityofamonobenzyltincomplexc1againstmdamb231cellsthroughinductionofapoptosisandinhibitionofbreastcancerstemcells AT hajiaghaalipourf anticanceractivityofamonobenzyltincomplexc1againstmdamb231cellsthroughinductionofapoptosisandinhibitionofbreastcancerstemcells AT rouhollahie anticanceractivityofamonobenzyltincomplexc1againstmdamb231cellsthroughinductionofapoptosisandinhibitionofbreastcancerstemcells AT hashimnm anticanceractivityofamonobenzyltincomplexc1againstmdamb231cellsthroughinductionofapoptosisandinhibitionofbreastcancerstemcells |