Inhibition of chikungunya virus replication by hesperetin and naringenin
Chikungunya virus (CHIKV) is an emerging arbovirus, which has recently become globally important. In recent years, it has posed a progressive threat to humankind, causing the development of possibly life-threatening and incapacitating arthritis. Currently, there is no available effective antiviral d...
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Royal Society of Chemistry
2016
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| author | Ahmadi, A. Hassandarvish, P. Lani, R. Yadollahi, P. Jokar, A. AbuBakar, Sazaly Zandi, Keivan |
| author_facet | Ahmadi, A. Hassandarvish, P. Lani, R. Yadollahi, P. Jokar, A. AbuBakar, Sazaly Zandi, Keivan |
| author_sort | Ahmadi, A. |
| collection | UM |
| description | Chikungunya virus (CHIKV) is an emerging arbovirus, which has recently become globally important. In recent years, it has posed a progressive threat to humankind, causing the development of possibly life-threatening and incapacitating arthritis. Currently, there is no available effective antiviral drug for chikungunya infection. Thus, finding and developing lead compounds with anti-CHIKV activity that could be further developed into a practical treatment is urgently required. Several studies have reported the wide-ranging antiviral activities of flavanones; however, an inhibitory effect of selected compounds has yet to be shown against CHIKV. In this study, we investigated the antiviral properties of two types of flavanones, namely naringenin and hesperetin, against CHIKV in vitro replication. Our data have shown dose dependent inhibitory effects for naringenin and hesperetin against CHIKV intracellular replication using different assays, including the CHIKV replicon cell line, time-of-addition and virus yield assays. The antiviral activity of the compounds was further investigated by the evaluation of CHIKV protein expression using a quantitative immunofluorescence assay and western blotting. In brief, these compounds presented significant antiviral activity against CHIKV, reducing both the CHIKV replication efficiency and down-regulating the production of viral proteins involved in replication. Naringenin with IC50 = 6.818 μM (SI = 80.27) and hesperetin with IC50 = 8.500 μM (SI = 23.34) inhibited the post entry stages of CHIKV replication activity. In conclusion, the obtained data from the current study suggest that naringenin and hesperetin could be potential candidates to be developed further as anti-CHIKV therapeutic agents. |
| first_indexed | 2024-03-06T05:44:33Z |
| format | Article |
| id | um.eprints-18138 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:44:33Z |
| publishDate | 2016 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | um.eprints-181382019-02-13T08:15:30Z http://eprints.um.edu.my/18138/ Inhibition of chikungunya virus replication by hesperetin and naringenin Ahmadi, A. Hassandarvish, P. Lani, R. Yadollahi, P. Jokar, A. AbuBakar, Sazaly Zandi, Keivan R Medicine Chikungunya virus (CHIKV) is an emerging arbovirus, which has recently become globally important. In recent years, it has posed a progressive threat to humankind, causing the development of possibly life-threatening and incapacitating arthritis. Currently, there is no available effective antiviral drug for chikungunya infection. Thus, finding and developing lead compounds with anti-CHIKV activity that could be further developed into a practical treatment is urgently required. Several studies have reported the wide-ranging antiviral activities of flavanones; however, an inhibitory effect of selected compounds has yet to be shown against CHIKV. In this study, we investigated the antiviral properties of two types of flavanones, namely naringenin and hesperetin, against CHIKV in vitro replication. Our data have shown dose dependent inhibitory effects for naringenin and hesperetin against CHIKV intracellular replication using different assays, including the CHIKV replicon cell line, time-of-addition and virus yield assays. The antiviral activity of the compounds was further investigated by the evaluation of CHIKV protein expression using a quantitative immunofluorescence assay and western blotting. In brief, these compounds presented significant antiviral activity against CHIKV, reducing both the CHIKV replication efficiency and down-regulating the production of viral proteins involved in replication. Naringenin with IC50 = 6.818 μM (SI = 80.27) and hesperetin with IC50 = 8.500 μM (SI = 23.34) inhibited the post entry stages of CHIKV replication activity. In conclusion, the obtained data from the current study suggest that naringenin and hesperetin could be potential candidates to be developed further as anti-CHIKV therapeutic agents. Royal Society of Chemistry 2016 Article PeerReviewed Ahmadi, A. and Hassandarvish, P. and Lani, R. and Yadollahi, P. and Jokar, A. and AbuBakar, Sazaly and Zandi, Keivan (2016) Inhibition of chikungunya virus replication by hesperetin and naringenin. RSC Advances, 6 (73). pp. 69421-69430. ISSN 2046-2069, DOI https://doi.org/10.1039/c6ra16640g <https://doi.org/10.1039/c6ra16640g>. https://doi.org/10.1039/c6ra16640g doi:10.1039/c6ra16640g |
| spellingShingle | R Medicine Ahmadi, A. Hassandarvish, P. Lani, R. Yadollahi, P. Jokar, A. AbuBakar, Sazaly Zandi, Keivan Inhibition of chikungunya virus replication by hesperetin and naringenin |
| title | Inhibition of chikungunya virus replication by hesperetin and naringenin |
| title_full | Inhibition of chikungunya virus replication by hesperetin and naringenin |
| title_fullStr | Inhibition of chikungunya virus replication by hesperetin and naringenin |
| title_full_unstemmed | Inhibition of chikungunya virus replication by hesperetin and naringenin |
| title_short | Inhibition of chikungunya virus replication by hesperetin and naringenin |
| title_sort | inhibition of chikungunya virus replication by hesperetin and naringenin |
| topic | R Medicine |
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