JMJD8 is a novel endoplasmic reticulum protein with a JmjC domain
Jumonji C (JmjC) domain-containing proteins have been shown to regulate cellular processes by hydroxylating or demethylating histone and non-histone targets. JMJD8 belongs to the JmjC domain-only family that was recently shown to be involved in angiogenesis and TNF-induced NF-κB signaling. Here, we...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Published: |
Nature Publishing Group
2017
|
| Subjects: |
| _version_ | 1825721202430181376 |
|---|---|
| author | Yeo, K.S. Tan, M.C. Lim, Y.Y. Ea, C.K. |
| author_facet | Yeo, K.S. Tan, M.C. Lim, Y.Y. Ea, C.K. |
| author_sort | Yeo, K.S. |
| collection | UM |
| description | Jumonji C (JmjC) domain-containing proteins have been shown to regulate cellular processes by hydroxylating or demethylating histone and non-histone targets. JMJD8 belongs to the JmjC domain-only family that was recently shown to be involved in angiogenesis and TNF-induced NF-κB signaling. Here, we employed bioinformatic analysis and immunofluorescence microscopy to examine the physiological properties of JMJD8. We demonstrated that JMJD8 localizes to the lumen of endoplasmic reticulum and that JMJD8 forms dimers or oligomers in vivo. Furthermore, we identified potential JMJD8-interacting proteins that are known to regulate protein complex assembly and protein folding. Taken together, this work demonstrates that JMJD8 is the first JmjC domain-containing protein found in the lumen of the endoplasmic reticulum that may function in protein complex assembly and protein folding. |
| first_indexed | 2024-03-06T05:46:48Z |
| format | Article |
| id | um.eprints-18941 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:46:48Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | um.eprints-189412018-07-30T08:00:24Z http://eprints.um.edu.my/18941/ JMJD8 is a novel endoplasmic reticulum protein with a JmjC domain Yeo, K.S. Tan, M.C. Lim, Y.Y. Ea, C.K. Q Science (General) QH Natural history Jumonji C (JmjC) domain-containing proteins have been shown to regulate cellular processes by hydroxylating or demethylating histone and non-histone targets. JMJD8 belongs to the JmjC domain-only family that was recently shown to be involved in angiogenesis and TNF-induced NF-κB signaling. Here, we employed bioinformatic analysis and immunofluorescence microscopy to examine the physiological properties of JMJD8. We demonstrated that JMJD8 localizes to the lumen of endoplasmic reticulum and that JMJD8 forms dimers or oligomers in vivo. Furthermore, we identified potential JMJD8-interacting proteins that are known to regulate protein complex assembly and protein folding. Taken together, this work demonstrates that JMJD8 is the first JmjC domain-containing protein found in the lumen of the endoplasmic reticulum that may function in protein complex assembly and protein folding. Nature Publishing Group 2017 Article PeerReviewed Yeo, K.S. and Tan, M.C. and Lim, Y.Y. and Ea, C.K. (2017) JMJD8 is a novel endoplasmic reticulum protein with a JmjC domain. Scientific Reports, 7 (1). p. 15407. ISSN 2045-2322, DOI https://doi.org/10.1038/s41598-017-15676-z <https://doi.org/10.1038/s41598-017-15676-z>. http://dx.doi.org/10.1038/s41598-017-15676-z doi:10.1038/s41598-017-15676-z |
| spellingShingle | Q Science (General) QH Natural history Yeo, K.S. Tan, M.C. Lim, Y.Y. Ea, C.K. JMJD8 is a novel endoplasmic reticulum protein with a JmjC domain |
| title | JMJD8 is a novel endoplasmic reticulum protein with a JmjC domain |
| title_full | JMJD8 is a novel endoplasmic reticulum protein with a JmjC domain |
| title_fullStr | JMJD8 is a novel endoplasmic reticulum protein with a JmjC domain |
| title_full_unstemmed | JMJD8 is a novel endoplasmic reticulum protein with a JmjC domain |
| title_short | JMJD8 is a novel endoplasmic reticulum protein with a JmjC domain |
| title_sort | jmjd8 is a novel endoplasmic reticulum protein with a jmjc domain |
| topic | Q Science (General) QH Natural history |
| work_keys_str_mv | AT yeoks jmjd8isanovelendoplasmicreticulumproteinwithajmjcdomain AT tanmc jmjd8isanovelendoplasmicreticulumproteinwithajmjcdomain AT limyy jmjd8isanovelendoplasmicreticulumproteinwithajmjcdomain AT eack jmjd8isanovelendoplasmicreticulumproteinwithajmjcdomain |