An in vitro potency assay using nicotinic acetylcholine receptor binding works well with antivenoms against Bungarus candidus and Naja naja
In order to facilitate/expedite the production of effective and affordable snake antivenoms, a novel in vitro potency assay was previously developed. The assay is based on an antiserum's ability to bind to postsynaptic neurotoxin (PSNT) and thereby inhibit the PSNT binding to the nicotinic acet...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2018
|
| Subjects: | |
| Online Access: | http://eprints.um.edu.my/18981/1/An_in_vitro_potency_assay_using_nicotinic_acetylcholine_receptor_binding_works_well_with_antivenoms_against_Bungarus_candidus_and_Naja_naja.pdf |
| _version_ | 1825721210301841408 |
|---|---|
| author | Ratanabanangkoon, K. Simsiriwong, P. Pruksaphon, K. Tan, K.Y. Chantrathonkul, B. Eursakun, S. Tan, C.H. |
| author_facet | Ratanabanangkoon, K. Simsiriwong, P. Pruksaphon, K. Tan, K.Y. Chantrathonkul, B. Eursakun, S. Tan, C.H. |
| author_sort | Ratanabanangkoon, K. |
| collection | UM |
| description | In order to facilitate/expedite the production of effective and affordable snake antivenoms, a novel in vitro potency assay was previously developed. The assay is based on an antiserum's ability to bind to postsynaptic neurotoxin (PSNT) and thereby inhibit the PSNT binding to the nicotinic acetylcholine receptor (nAChR). The assay was shown to work well with antiserum against Thai Naja kaouthia which produces predominantly the lethal PSNTs. In this work, the assay is demonstrated to work well with antiserum/antivenom against Bungarus candidus (BC), which also produces lethal presynaptic neurotoxins, as well as antivenom against Sri Lankan Naja naja (NN), which produces an abundance of cytotoxins. The in vitro and in vivo median effective ratios (ER50s) for various batches of antisera against BC showed a correlation (R 2) of 0.8922 (p < 0.001) while the corresponding value for the anti-NN antivenom was R 2 = 0.7898 (p < 0.01). These results, together with the known toxin profiles of various genera of elapids, suggest that this in vitro assay could be used with antisera against other species of Bungarus and Naja and possibly other neurotoxic snake venoms worldwide. The assay should significantly save numerous lives of mice and accelerate production of life-saving antivenoms. |
| first_indexed | 2024-03-06T05:46:55Z |
| format | Article |
| id | um.eprints-18981 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:46:55Z |
| publishDate | 2018 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | um.eprints-189812018-08-07T08:32:53Z http://eprints.um.edu.my/18981/ An in vitro potency assay using nicotinic acetylcholine receptor binding works well with antivenoms against Bungarus candidus and Naja naja Ratanabanangkoon, K. Simsiriwong, P. Pruksaphon, K. Tan, K.Y. Chantrathonkul, B. Eursakun, S. Tan, C.H. R Medicine In order to facilitate/expedite the production of effective and affordable snake antivenoms, a novel in vitro potency assay was previously developed. The assay is based on an antiserum's ability to bind to postsynaptic neurotoxin (PSNT) and thereby inhibit the PSNT binding to the nicotinic acetylcholine receptor (nAChR). The assay was shown to work well with antiserum against Thai Naja kaouthia which produces predominantly the lethal PSNTs. In this work, the assay is demonstrated to work well with antiserum/antivenom against Bungarus candidus (BC), which also produces lethal presynaptic neurotoxins, as well as antivenom against Sri Lankan Naja naja (NN), which produces an abundance of cytotoxins. The in vitro and in vivo median effective ratios (ER50s) for various batches of antisera against BC showed a correlation (R 2) of 0.8922 (p < 0.001) while the corresponding value for the anti-NN antivenom was R 2 = 0.7898 (p < 0.01). These results, together with the known toxin profiles of various genera of elapids, suggest that this in vitro assay could be used with antisera against other species of Bungarus and Naja and possibly other neurotoxic snake venoms worldwide. The assay should significantly save numerous lives of mice and accelerate production of life-saving antivenoms. Nature Publishing Group 2018 Article PeerReviewed application/pdf en http://eprints.um.edu.my/18981/1/An_in_vitro_potency_assay_using_nicotinic_acetylcholine_receptor_binding_works_well_with_antivenoms_against_Bungarus_candidus_and_Naja_naja.pdf Ratanabanangkoon, K. and Simsiriwong, P. and Pruksaphon, K. and Tan, K.Y. and Chantrathonkul, B. and Eursakun, S. and Tan, C.H. (2018) An in vitro potency assay using nicotinic acetylcholine receptor binding works well with antivenoms against Bungarus candidus and Naja naja. Scientific Reports, 8 (1). p. 9716. ISSN 2045-2322, DOI https://doi.org/10.1038/s41598-018-27794-3 <https://doi.org/10.1038/s41598-018-27794-3>. http://dx.doi.org/10.1038/s41598-018-27794-3 doi:10.1038/s41598-018-27794-3 |
| spellingShingle | R Medicine Ratanabanangkoon, K. Simsiriwong, P. Pruksaphon, K. Tan, K.Y. Chantrathonkul, B. Eursakun, S. Tan, C.H. An in vitro potency assay using nicotinic acetylcholine receptor binding works well with antivenoms against Bungarus candidus and Naja naja |
| title | An in vitro potency assay using nicotinic acetylcholine receptor binding works well with antivenoms against Bungarus candidus and Naja naja |
| title_full | An in vitro potency assay using nicotinic acetylcholine receptor binding works well with antivenoms against Bungarus candidus and Naja naja |
| title_fullStr | An in vitro potency assay using nicotinic acetylcholine receptor binding works well with antivenoms against Bungarus candidus and Naja naja |
| title_full_unstemmed | An in vitro potency assay using nicotinic acetylcholine receptor binding works well with antivenoms against Bungarus candidus and Naja naja |
| title_short | An in vitro potency assay using nicotinic acetylcholine receptor binding works well with antivenoms against Bungarus candidus and Naja naja |
| title_sort | in vitro potency assay using nicotinic acetylcholine receptor binding works well with antivenoms against bungarus candidus and naja naja |
| topic | R Medicine |
| url | http://eprints.um.edu.my/18981/1/An_in_vitro_potency_assay_using_nicotinic_acetylcholine_receptor_binding_works_well_with_antivenoms_against_Bungarus_candidus_and_Naja_naja.pdf |
| work_keys_str_mv | AT ratanabanangkoonk aninvitropotencyassayusingnicotinicacetylcholinereceptorbindingworkswellwithantivenomsagainstbungaruscandidusandnajanaja AT simsiriwongp aninvitropotencyassayusingnicotinicacetylcholinereceptorbindingworkswellwithantivenomsagainstbungaruscandidusandnajanaja AT pruksaphonk aninvitropotencyassayusingnicotinicacetylcholinereceptorbindingworkswellwithantivenomsagainstbungaruscandidusandnajanaja AT tanky aninvitropotencyassayusingnicotinicacetylcholinereceptorbindingworkswellwithantivenomsagainstbungaruscandidusandnajanaja AT chantrathonkulb aninvitropotencyassayusingnicotinicacetylcholinereceptorbindingworkswellwithantivenomsagainstbungaruscandidusandnajanaja AT eursakuns aninvitropotencyassayusingnicotinicacetylcholinereceptorbindingworkswellwithantivenomsagainstbungaruscandidusandnajanaja AT tanch aninvitropotencyassayusingnicotinicacetylcholinereceptorbindingworkswellwithantivenomsagainstbungaruscandidusandnajanaja AT ratanabanangkoonk invitropotencyassayusingnicotinicacetylcholinereceptorbindingworkswellwithantivenomsagainstbungaruscandidusandnajanaja AT simsiriwongp invitropotencyassayusingnicotinicacetylcholinereceptorbindingworkswellwithantivenomsagainstbungaruscandidusandnajanaja AT pruksaphonk invitropotencyassayusingnicotinicacetylcholinereceptorbindingworkswellwithantivenomsagainstbungaruscandidusandnajanaja AT tanky invitropotencyassayusingnicotinicacetylcholinereceptorbindingworkswellwithantivenomsagainstbungaruscandidusandnajanaja AT chantrathonkulb invitropotencyassayusingnicotinicacetylcholinereceptorbindingworkswellwithantivenomsagainstbungaruscandidusandnajanaja AT eursakuns invitropotencyassayusingnicotinicacetylcholinereceptorbindingworkswellwithantivenomsagainstbungaruscandidusandnajanaja AT tanch invitropotencyassayusingnicotinicacetylcholinereceptorbindingworkswellwithantivenomsagainstbungaruscandidusandnajanaja |