Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology
Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was p...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI
2017
|
| Subjects: | |
| Online Access: | http://eprints.um.edu.my/19035/1/Optimization_of_Reducing_Sugar_Production_from_Manihot_glaziovii_Starch_Using_Response_Surface_Methodology.pdf |
| _version_ | 1796960600942706688 |
|---|---|
| author | Sebayang, A.H. Masjuki, Haji Hassan Ong, H.C. Dharma, S. Silitonga, A.S. Kusumo, F. Mahlia, T.M.I. Bahar, A.H. |
| author_facet | Sebayang, A.H. Masjuki, Haji Hassan Ong, H.C. Dharma, S. Silitonga, A.S. Kusumo, F. Mahlia, T.M.I. Bahar, A.H. |
| author_sort | Sebayang, A.H. |
| collection | UM |
| description | Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was performed with selected hydrolysis parameters, including substrate loading, stroke speed, α-amylase concentration and amyloglucosidase concentration. From the experiment, the resulting optimum conditions are 23.88% (w/v) substrate loading, 109.43 U/g α-amylase concentration, 65.44 U/mL amyloglucosidase concentration and 74.87 rpm stroke speed, which yielded 196.23 g/L reducing sugar. The fermentation process was also carried out, with a production value of 0.45 g ethanol/g reducing sugar, which is equivalent to 88.61% of ethanol yield after fermentation by using Saccharomyces cerevisiae (S. cerevisiae). The physical and chemical properties of the produced ethanol are within the specifications of the ASTM D4806 standard. The good quality of ethanol produced from this study indicates that Manihot glaziovii (M. glaziovii) has great potential as bioethanol feedstock. |
| first_indexed | 2024-03-06T05:47:04Z |
| format | Article |
| id | um.eprints-19035 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:47:04Z |
| publishDate | 2017 |
| publisher | MDPI |
| record_format | dspace |
| spelling | um.eprints-190352018-10-16T05:22:30Z http://eprints.um.edu.my/19035/ Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology Sebayang, A.H. Masjuki, Haji Hassan Ong, H.C. Dharma, S. Silitonga, A.S. Kusumo, F. Mahlia, T.M.I. Bahar, A.H. TJ Mechanical engineering and machinery Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was performed with selected hydrolysis parameters, including substrate loading, stroke speed, α-amylase concentration and amyloglucosidase concentration. From the experiment, the resulting optimum conditions are 23.88% (w/v) substrate loading, 109.43 U/g α-amylase concentration, 65.44 U/mL amyloglucosidase concentration and 74.87 rpm stroke speed, which yielded 196.23 g/L reducing sugar. The fermentation process was also carried out, with a production value of 0.45 g ethanol/g reducing sugar, which is equivalent to 88.61% of ethanol yield after fermentation by using Saccharomyces cerevisiae (S. cerevisiae). The physical and chemical properties of the produced ethanol are within the specifications of the ASTM D4806 standard. The good quality of ethanol produced from this study indicates that Manihot glaziovii (M. glaziovii) has great potential as bioethanol feedstock. MDPI 2017 Article PeerReviewed application/pdf en http://eprints.um.edu.my/19035/1/Optimization_of_Reducing_Sugar_Production_from_Manihot_glaziovii_Starch_Using_Response_Surface_Methodology.pdf Sebayang, A.H. and Masjuki, Haji Hassan and Ong, H.C. and Dharma, S. and Silitonga, A.S. and Kusumo, F. and Mahlia, T.M.I. and Bahar, A.H. (2017) Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology. Energies, 10 (1). p. 35. ISSN 1996-1073, DOI https://doi.org/10.3390/en10010035 <https://doi.org/10.3390/en10010035>. http://dx.doi.org/10.3390/en10010035 doi:10.3390/en10010035 |
| spellingShingle | TJ Mechanical engineering and machinery Sebayang, A.H. Masjuki, Haji Hassan Ong, H.C. Dharma, S. Silitonga, A.S. Kusumo, F. Mahlia, T.M.I. Bahar, A.H. Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology |
| title | Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology |
| title_full | Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology |
| title_fullStr | Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology |
| title_full_unstemmed | Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology |
| title_short | Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology |
| title_sort | optimization of reducing sugar production from manihot glaziovii starch using response surface methodology |
| topic | TJ Mechanical engineering and machinery |
| url | http://eprints.um.edu.my/19035/1/Optimization_of_Reducing_Sugar_Production_from_Manihot_glaziovii_Starch_Using_Response_Surface_Methodology.pdf |
| work_keys_str_mv | AT sebayangah optimizationofreducingsugarproductionfrommanihotglazioviistarchusingresponsesurfacemethodology AT masjukihajihassan optimizationofreducingsugarproductionfrommanihotglazioviistarchusingresponsesurfacemethodology AT onghc optimizationofreducingsugarproductionfrommanihotglazioviistarchusingresponsesurfacemethodology AT dharmas optimizationofreducingsugarproductionfrommanihotglazioviistarchusingresponsesurfacemethodology AT silitongaas optimizationofreducingsugarproductionfrommanihotglazioviistarchusingresponsesurfacemethodology AT kusumof optimizationofreducingsugarproductionfrommanihotglazioviistarchusingresponsesurfacemethodology AT mahliatmi optimizationofreducingsugarproductionfrommanihotglazioviistarchusingresponsesurfacemethodology AT baharah optimizationofreducingsugarproductionfrommanihotglazioviistarchusingresponsesurfacemethodology |