Alcohol-induced structural transitions in the acid-denatured Bacillus licheniformis α-amylase
Alcohol-induced structural changes in the acid-denatured Bacillus licheniformis α-amylase (BLA) at pH 2.0 were studied by far-ultra violet circular dichroism, intrinsic, three-dimensional and 8-anilino-1-naphthalene sulfonic acid (ANS) fluorescence, acrylamide quenching and thermal denaturation. All...
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| Format: | Article |
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Elsevier
2017
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| author | Halim, A.A.A. Zaroog, M.S. Kadir, H.A. Tayyab, S. |
| author_facet | Halim, A.A.A. Zaroog, M.S. Kadir, H.A. Tayyab, S. |
| author_sort | Halim, A.A.A. |
| collection | UM |
| description | Alcohol-induced structural changes in the acid-denatured Bacillus licheniformis α-amylase (BLA) at pH 2.0 were studied by far-ultra violet circular dichroism, intrinsic, three-dimensional and 8-anilino-1-naphthalene sulfonic acid (ANS) fluorescence, acrylamide quenching and thermal denaturation. All the alcohols used in this study produced partial refolding in the acid-denatured BLA as evident from the increased mean residue ellipticity at 222 nm, increased intrinsic fluorescence and decreased ANS fluorescence. The order of effectiveness of these alcohols to induce a partially folded state of BLA was found to be: 2,2,2-trifluoroethanol/tert-butanol > 1-propanol/2-propanol > 2-chloroethanol > ethanol > methanol. Three-dimensional fluorescence and acrylamide quenching results obtained in the presence of 5.5 M tert-butanol also suggested formation of a partially folded state in the acid-denatured BLA. However, 5.5 M tert-butanol-induced state of BLA showed a non-cooperative thermal transition. All these results suggested formation of a partially folded state of the acid-denatured BLA in the presence of these alcohols. Furthermore, their effectiveness was found to be guided by their chain length, position of methyl groups and presence of the substituents. |
| first_indexed | 2024-03-06T05:47:30Z |
| format | Article |
| id | um.eprints-19178 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:47:30Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | um.eprints-191782018-09-07T02:18:00Z http://eprints.um.edu.my/19178/ Alcohol-induced structural transitions in the acid-denatured Bacillus licheniformis α-amylase Halim, A.A.A. Zaroog, M.S. Kadir, H.A. Tayyab, S. Q Science (General) QD Chemistry QH Natural history Alcohol-induced structural changes in the acid-denatured Bacillus licheniformis α-amylase (BLA) at pH 2.0 were studied by far-ultra violet circular dichroism, intrinsic, three-dimensional and 8-anilino-1-naphthalene sulfonic acid (ANS) fluorescence, acrylamide quenching and thermal denaturation. All the alcohols used in this study produced partial refolding in the acid-denatured BLA as evident from the increased mean residue ellipticity at 222 nm, increased intrinsic fluorescence and decreased ANS fluorescence. The order of effectiveness of these alcohols to induce a partially folded state of BLA was found to be: 2,2,2-trifluoroethanol/tert-butanol > 1-propanol/2-propanol > 2-chloroethanol > ethanol > methanol. Three-dimensional fluorescence and acrylamide quenching results obtained in the presence of 5.5 M tert-butanol also suggested formation of a partially folded state in the acid-denatured BLA. However, 5.5 M tert-butanol-induced state of BLA showed a non-cooperative thermal transition. All these results suggested formation of a partially folded state of the acid-denatured BLA in the presence of these alcohols. Furthermore, their effectiveness was found to be guided by their chain length, position of methyl groups and presence of the substituents. Elsevier 2017 Article PeerReviewed Halim, A.A.A. and Zaroog, M.S. and Kadir, H.A. and Tayyab, S. (2017) Alcohol-induced structural transitions in the acid-denatured Bacillus licheniformis α-amylase. Journal of Saudi Chemical Society, 21 (Spp.1). S349-S358. ISSN 1319-6103, DOI https://doi.org/10.1016/j.jscs.2014.04.002 <https://doi.org/10.1016/j.jscs.2014.04.002>. http://dx.doi.org/10.1016/j.jscs.2014.04.002 doi:10.1016/j.jscs.2014.04.002 |
| spellingShingle | Q Science (General) QD Chemistry QH Natural history Halim, A.A.A. Zaroog, M.S. Kadir, H.A. Tayyab, S. Alcohol-induced structural transitions in the acid-denatured Bacillus licheniformis α-amylase |
| title | Alcohol-induced structural transitions in the acid-denatured Bacillus licheniformis α-amylase |
| title_full | Alcohol-induced structural transitions in the acid-denatured Bacillus licheniformis α-amylase |
| title_fullStr | Alcohol-induced structural transitions in the acid-denatured Bacillus licheniformis α-amylase |
| title_full_unstemmed | Alcohol-induced structural transitions in the acid-denatured Bacillus licheniformis α-amylase |
| title_short | Alcohol-induced structural transitions in the acid-denatured Bacillus licheniformis α-amylase |
| title_sort | alcohol induced structural transitions in the acid denatured bacillus licheniformis α amylase |
| topic | Q Science (General) QD Chemistry QH Natural history |
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