Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling
With a diverse host range, Meloidogyne incognita (root-knot nematode) is listed as one of the most economically important obligate parasites of agriculture. This nematode species establishes permanent feeding sites in plant root systems soon after infestation. A compatible host-nematode interaction...
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| Format: | Article |
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Public Library of Science
2017
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| _version_ | 1796960642717974528 |
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| author | Al-Idrus, A. Carpentier, S.C. Ahmad, M.T. Panis, B. Mohamed, Z. |
| author_facet | Al-Idrus, A. Carpentier, S.C. Ahmad, M.T. Panis, B. Mohamed, Z. |
| author_sort | Al-Idrus, A. |
| collection | UM |
| description | With a diverse host range, Meloidogyne incognita (root-knot nematode) is listed as one of the most economically important obligate parasites of agriculture. This nematode species establishes permanent feeding sites in plant root systems soon after infestation. A compatible host-nematode interaction triggers a cascade of morphological and physiological process disruptions of the host, leading to pathogenesis. Such disruption is reflected by altered gene expression in affected cells, detectable using molecular approaches. We employed a high-throughput proteomics approach to elucidate the events involved in a compatible banana-M. incognita interaction. This study serves as the first crucial step in developing natural banana resistance for the purpose of biological-based nematode management programme. We successfully profiled 114 Grand naine root proteins involved in the interaction with M. incognita at the 30th- and 60th- day after inoculation (dai). The abundance of proteins involved in fundamental biological processes, cellular component organisation and stress responses were significantly altered in inoculated root samples. In addition, the abundance of proteins in pathways associated with defence and giant cell maintenance in plants such as phenylpropanoid biosynthesis, glycolysis and citrate cycle were also implicated by the infestation. |
| first_indexed | 2024-03-06T05:47:43Z |
| format | Article |
| id | um.eprints-19245 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:47:43Z |
| publishDate | 2017 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | um.eprints-192452018-09-18T04:22:48Z http://eprints.um.edu.my/19245/ Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling Al-Idrus, A. Carpentier, S.C. Ahmad, M.T. Panis, B. Mohamed, Z. Q Science (General) QH Natural history With a diverse host range, Meloidogyne incognita (root-knot nematode) is listed as one of the most economically important obligate parasites of agriculture. This nematode species establishes permanent feeding sites in plant root systems soon after infestation. A compatible host-nematode interaction triggers a cascade of morphological and physiological process disruptions of the host, leading to pathogenesis. Such disruption is reflected by altered gene expression in affected cells, detectable using molecular approaches. We employed a high-throughput proteomics approach to elucidate the events involved in a compatible banana-M. incognita interaction. This study serves as the first crucial step in developing natural banana resistance for the purpose of biological-based nematode management programme. We successfully profiled 114 Grand naine root proteins involved in the interaction with M. incognita at the 30th- and 60th- day after inoculation (dai). The abundance of proteins involved in fundamental biological processes, cellular component organisation and stress responses were significantly altered in inoculated root samples. In addition, the abundance of proteins in pathways associated with defence and giant cell maintenance in plants such as phenylpropanoid biosynthesis, glycolysis and citrate cycle were also implicated by the infestation. Public Library of Science 2017 Article PeerReviewed Al-Idrus, A. and Carpentier, S.C. and Ahmad, M.T. and Panis, B. and Mohamed, Z. (2017) Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling. PLoS ONE, 12 (6). e0178438. ISSN 1932-6203, DOI https://doi.org/10.1371/journal.pone.0178438 <https://doi.org/10.1371/journal.pone.0178438>. http://dx.doi.org/10.1371/journal.pone.0178438 doi:10.1371/journal.pone.0178438 |
| spellingShingle | Q Science (General) QH Natural history Al-Idrus, A. Carpentier, S.C. Ahmad, M.T. Panis, B. Mohamed, Z. Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling |
| title | Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling |
| title_full | Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling |
| title_fullStr | Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling |
| title_full_unstemmed | Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling |
| title_short | Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling |
| title_sort | elucidation of the compatible interaction between banana and meloidogyne incognita via high throughput proteome profiling |
| topic | Q Science (General) QH Natural history |
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