Intrinsic Fluorescence as a Spectral Probe for Protein Denaturation Studies in the Presence of Honey
Honey was found to quench the intrinsic fluorescence of bovine serum albumin (BSA) in a concentration dependent manner, showing complete quenching in the presence of 5% (w/v) honey. Increasing the protein concentration up to 5.0 μM did not lead to the recovery of the protein fluorescence. Urea denat...
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| Format: | Article |
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Kluwer (now part of Springer)
2015
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| _version_ | 1796960679645675520 |
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| author | Wong, Y.H. Kadir, H.A. Tayyab, S. |
| author_facet | Wong, Y.H. Kadir, H.A. Tayyab, S. |
| author_sort | Wong, Y.H. |
| collection | UM |
| description | Honey was found to quench the intrinsic fluorescence of bovine serum albumin (BSA) in a concentration dependent manner, showing complete quenching in the presence of 5% (w/v) honey. Increasing the protein concentration up to 5.0 μM did not lead to the recovery of the protein fluorescence. Urea denaturation of BSA, which otherwise shows a two-step, three-state transition, using intrinsic fluorescence of the protein as the probe failed to produce any result in the presence of 5% (w/v) honey. Thus, intrinsic fluorescence cannot be used as a spectral probe for protein denaturation studies in the presence of honey. |
| first_indexed | 2024-03-06T05:48:16Z |
| format | Article |
| id | um.eprints-19431 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:48:16Z |
| publishDate | 2015 |
| publisher | Kluwer (now part of Springer) |
| record_format | dspace |
| spelling | um.eprints-194312018-09-26T07:42:16Z http://eprints.um.edu.my/19431/ Intrinsic Fluorescence as a Spectral Probe for Protein Denaturation Studies in the Presence of Honey Wong, Y.H. Kadir, H.A. Tayyab, S. Q Science (General) QH Natural history Honey was found to quench the intrinsic fluorescence of bovine serum albumin (BSA) in a concentration dependent manner, showing complete quenching in the presence of 5% (w/v) honey. Increasing the protein concentration up to 5.0 μM did not lead to the recovery of the protein fluorescence. Urea denaturation of BSA, which otherwise shows a two-step, three-state transition, using intrinsic fluorescence of the protein as the probe failed to produce any result in the presence of 5% (w/v) honey. Thus, intrinsic fluorescence cannot be used as a spectral probe for protein denaturation studies in the presence of honey. Kluwer (now part of Springer) 2015 Article PeerReviewed Wong, Y.H. and Kadir, H.A. and Tayyab, S. (2015) Intrinsic Fluorescence as a Spectral Probe for Protein Denaturation Studies in the Presence of Honey. Journal of Applied Spectroscopy, 82 (5). pp. 845-848. ISSN 0021-9037, DOI https://doi.org/10.1007/s10812-015-0191-z <https://doi.org/10.1007/s10812-015-0191-z>. http://dx.doi.org/10.1007/s10812-015-0191-z doi:10.1007/s10812-015-0191-z |
| spellingShingle | Q Science (General) QH Natural history Wong, Y.H. Kadir, H.A. Tayyab, S. Intrinsic Fluorescence as a Spectral Probe for Protein Denaturation Studies in the Presence of Honey |
| title | Intrinsic Fluorescence as a Spectral Probe for Protein Denaturation Studies in the Presence of Honey |
| title_full | Intrinsic Fluorescence as a Spectral Probe for Protein Denaturation Studies in the Presence of Honey |
| title_fullStr | Intrinsic Fluorescence as a Spectral Probe for Protein Denaturation Studies in the Presence of Honey |
| title_full_unstemmed | Intrinsic Fluorescence as a Spectral Probe for Protein Denaturation Studies in the Presence of Honey |
| title_short | Intrinsic Fluorescence as a Spectral Probe for Protein Denaturation Studies in the Presence of Honey |
| title_sort | intrinsic fluorescence as a spectral probe for protein denaturation studies in the presence of honey |
| topic | Q Science (General) QH Natural history |
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