A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity
Copper ion recognition and DNA interaction of a newly synthesized fluorescent Schiff base (HPyETSC) were investigated using UV-vis and fluorescent spectroscopy. Examination using these two techniques revealed that the detection of copper by HPyETSC is highly sensitive and selective, with a detection...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
Elsevier
2015
|
| Subjects: |
| _version_ | 1825721309989961728 |
|---|---|
| author | Vikneswaran, R. Syafiq, M.S. Eltayeb, N.E. Kamaruddin, M.N. Ramesh, Subramaniam Yahya, Rosiyah |
| author_facet | Vikneswaran, R. Syafiq, M.S. Eltayeb, N.E. Kamaruddin, M.N. Ramesh, Subramaniam Yahya, Rosiyah |
| author_sort | Vikneswaran, R. |
| collection | UM |
| description | Copper ion recognition and DNA interaction of a newly synthesized fluorescent Schiff base (HPyETSC) were investigated using UV-vis and fluorescent spectroscopy. Examination using these two techniques revealed that the detection of copper by HPyETSC is highly sensitive and selective, with a detection limit of 0.39 μm and the mode of interaction between HPyETSC and DNA is electrostatic, with a binding constant of 8.97 × 104 M-1. Furthermore, gel electrophoresis studies showed that HPyETSC exhibited nuclease activity through oxidative pathway. |
| first_indexed | 2024-03-06T05:48:27Z |
| format | Article |
| id | um.eprints-19492 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:48:27Z |
| publishDate | 2015 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | um.eprints-194922019-03-18T08:30:08Z http://eprints.um.edu.my/19492/ A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity Vikneswaran, R. Syafiq, M.S. Eltayeb, N.E. Kamaruddin, M.N. Ramesh, Subramaniam Yahya, Rosiyah Q Science (General) QC Physics QD Chemistry Copper ion recognition and DNA interaction of a newly synthesized fluorescent Schiff base (HPyETSC) were investigated using UV-vis and fluorescent spectroscopy. Examination using these two techniques revealed that the detection of copper by HPyETSC is highly sensitive and selective, with a detection limit of 0.39 μm and the mode of interaction between HPyETSC and DNA is electrostatic, with a binding constant of 8.97 × 104 M-1. Furthermore, gel electrophoresis studies showed that HPyETSC exhibited nuclease activity through oxidative pathway. Elsevier 2015 Article PeerReviewed Vikneswaran, R. and Syafiq, M.S. and Eltayeb, N.E. and Kamaruddin, M.N. and Ramesh, Subramaniam and Yahya, Rosiyah (2015) A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 150. pp. 175-180. ISSN 1386-1425, DOI https://doi.org/10.1016/j.saa.2015.05.087 <https://doi.org/10.1016/j.saa.2015.05.087>. http://dx.doi.org/10.1016/j.saa.2015.05.087 doi:10.1016/j.saa.2015.05.087 |
| spellingShingle | Q Science (General) QC Physics QD Chemistry Vikneswaran, R. Syafiq, M.S. Eltayeb, N.E. Kamaruddin, M.N. Ramesh, Subramaniam Yahya, Rosiyah A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity |
| title | A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity |
| title_full | A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity |
| title_fullStr | A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity |
| title_full_unstemmed | A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity |
| title_short | A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity |
| title_sort | new thio schiff base fluorophore with copper ion sensing dna binding and nuclease activity |
| topic | Q Science (General) QC Physics QD Chemistry |
| work_keys_str_mv | AT vikneswaranr anewthioschiffbasefluorophorewithcopperionsensingdnabindingandnucleaseactivity AT syafiqms anewthioschiffbasefluorophorewithcopperionsensingdnabindingandnucleaseactivity AT eltayebne anewthioschiffbasefluorophorewithcopperionsensingdnabindingandnucleaseactivity AT kamaruddinmn anewthioschiffbasefluorophorewithcopperionsensingdnabindingandnucleaseactivity AT rameshsubramaniam anewthioschiffbasefluorophorewithcopperionsensingdnabindingandnucleaseactivity AT yahyarosiyah anewthioschiffbasefluorophorewithcopperionsensingdnabindingandnucleaseactivity AT vikneswaranr newthioschiffbasefluorophorewithcopperionsensingdnabindingandnucleaseactivity AT syafiqms newthioschiffbasefluorophorewithcopperionsensingdnabindingandnucleaseactivity AT eltayebne newthioschiffbasefluorophorewithcopperionsensingdnabindingandnucleaseactivity AT kamaruddinmn newthioschiffbasefluorophorewithcopperionsensingdnabindingandnucleaseactivity AT rameshsubramaniam newthioschiffbasefluorophorewithcopperionsensingdnabindingandnucleaseactivity AT yahyarosiyah newthioschiffbasefluorophorewithcopperionsensingdnabindingandnucleaseactivity |