Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route
Natural biopolymers have many attractive medical applications; however, complications due to fibrosis caused a reduction in diffusion and dispersal of nutrients and waste products. Consequently, severe immunocompatibility problems and poor mechanical and degradation properties in synthetic polymers...
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| Format: | Article |
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SAGE Publications
2019
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| author | Latfi, Ahmad Safwan Ahmad Pramanik, Sumit Poon, Chi Tat Gumel, Ahmad Mohammed Lai, Khin Wee Annuar, Mohamad Suffian Mohamad Pingguan-Murphy, Belinda |
| author_facet | Latfi, Ahmad Safwan Ahmad Pramanik, Sumit Poon, Chi Tat Gumel, Ahmad Mohammed Lai, Khin Wee Annuar, Mohamad Suffian Mohamad Pingguan-Murphy, Belinda |
| author_sort | Latfi, Ahmad Safwan Ahmad |
| collection | UM |
| description | Natural biopolymers have many attractive medical applications; however, complications due to fibrosis caused a reduction in diffusion and dispersal of nutrients and waste products. Consequently, severe immunocompatibility problems and poor mechanical and degradation properties in synthetic polymers ensue. Hence, the present study investigates a novel hydrogel material synthesized from caprolactone, ethylene glycol, ethylenediamine, polyethylene glycol, ammonium persulfate, and tetramethylethylenediamine via chemo-enzymatic route. Spectroscopic analyses indicated the formation of polyurea and polyhydroxyurethane as the primary building block of the hydrogel starting material. Biocompatibility studies showed positive observation in biosafety test using direct contact cytotoxicity assay in addition to active cellular growth on the hydrogel scaffold based on fluorescence observation. The synthesized hydrogel also exhibited (self)fluorescence properties under specific wavelength excitation. Hence, synthesized hydrogel could be a potential candidate for medical imaging as well as tissue engineering applications as a tissue expander, coating material, biosensor, and drug delivery system. |
| first_indexed | 2024-03-06T05:50:02Z |
| format | Article |
| id | um.eprints-20041 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:50:02Z |
| publishDate | 2019 |
| publisher | SAGE Publications |
| record_format | dspace |
| spelling | um.eprints-200412019-02-27T00:45:51Z http://eprints.um.edu.my/20041/ Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route Latfi, Ahmad Safwan Ahmad Pramanik, Sumit Poon, Chi Tat Gumel, Ahmad Mohammed Lai, Khin Wee Annuar, Mohamad Suffian Mohamad Pingguan-Murphy, Belinda Q Science (General) QH Natural history R Medicine Natural biopolymers have many attractive medical applications; however, complications due to fibrosis caused a reduction in diffusion and dispersal of nutrients and waste products. Consequently, severe immunocompatibility problems and poor mechanical and degradation properties in synthetic polymers ensue. Hence, the present study investigates a novel hydrogel material synthesized from caprolactone, ethylene glycol, ethylenediamine, polyethylene glycol, ammonium persulfate, and tetramethylethylenediamine via chemo-enzymatic route. Spectroscopic analyses indicated the formation of polyurea and polyhydroxyurethane as the primary building block of the hydrogel starting material. Biocompatibility studies showed positive observation in biosafety test using direct contact cytotoxicity assay in addition to active cellular growth on the hydrogel scaffold based on fluorescence observation. The synthesized hydrogel also exhibited (self)fluorescence properties under specific wavelength excitation. Hence, synthesized hydrogel could be a potential candidate for medical imaging as well as tissue engineering applications as a tissue expander, coating material, biosensor, and drug delivery system. SAGE Publications 2019 Article PeerReviewed Latfi, Ahmad Safwan Ahmad and Pramanik, Sumit and Poon, Chi Tat and Gumel, Ahmad Mohammed and Lai, Khin Wee and Annuar, Mohamad Suffian Mohamad and Pingguan-Murphy, Belinda (2019) Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route. Journal of Biomaterials Applications, 33 (6). pp. 854-865. ISSN 0885-3282, DOI https://doi.org/10.1177/0885328218812490 <https://doi.org/10.1177/0885328218812490>. https://doi.org/10.1177/0885328218812490 doi:10.1177/0885328218812490 |
| spellingShingle | Q Science (General) QH Natural history R Medicine Latfi, Ahmad Safwan Ahmad Pramanik, Sumit Poon, Chi Tat Gumel, Ahmad Mohammed Lai, Khin Wee Annuar, Mohamad Suffian Mohamad Pingguan-Murphy, Belinda Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route |
| title | Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route |
| title_full | Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route |
| title_fullStr | Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route |
| title_full_unstemmed | Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route |
| title_short | Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route |
| title_sort | structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo enzymatic route |
| topic | Q Science (General) QH Natural history R Medicine |
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