Bioethanol production from agarophyte red seaweed, Gelidium elegans, using a novel sample preparation method for analysing bioethanol content by gas chromatography
In this study, Gelidium elegans is investigated for ethanol production. A combination of factors including different temperatures, acid concentration and incubation time was evaluated to determine the suitable saccharification conditions. The combination of 2.5% (w/v) H2SO4 at 120 °C for 40 min was...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Published: |
Springer Verlag (Germany)
2019
|
| Subjects: |
| _version_ | 1796960809021079552 |
|---|---|
| author | Hessami, Mohammad Javad Cheng, Sit Foon Ambati, Ranga Rao Yeong, Hui Yin Phang, Siew Moi |
| author_facet | Hessami, Mohammad Javad Cheng, Sit Foon Ambati, Ranga Rao Yeong, Hui Yin Phang, Siew Moi |
| author_sort | Hessami, Mohammad Javad |
| collection | UM |
| description | In this study, Gelidium elegans is investigated for ethanol production. A combination of factors including different temperatures, acid concentration and incubation time was evaluated to determine the suitable saccharification conditions. The combination of 2.5% (w/v) H2SO4 at 120 °C for 40 min was selected for hydrolysis of the seaweed biomass, followed by purification, and fermentation to yield ethanol. The galactose and glucose were dominant reducing sugars in the G. elegans hydrolysate and under optimum condition of dilute acid hydrolysis, 39.42% of reducing sugars was produced and fermentation resulted in ethanol concentration of 13.27 ± 0.47 g/L. A modified method was evaluated for sample preparation for gas chromatography (GC) analysis of the ethanol content. A solvent mixture of acetonitrile and iso-butanol precipitated dissolved organic residues and reduced water content in GC samples at least by 90%. Results showed that this method could be successfully used for bioethanol production from seaweed. |
| first_indexed | 2024-03-06T05:50:07Z |
| format | Article |
| id | um.eprints-20073 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:50:07Z |
| publishDate | 2019 |
| publisher | Springer Verlag (Germany) |
| record_format | dspace |
| spelling | um.eprints-200732019-01-22T01:47:10Z http://eprints.um.edu.my/20073/ Bioethanol production from agarophyte red seaweed, Gelidium elegans, using a novel sample preparation method for analysing bioethanol content by gas chromatography Hessami, Mohammad Javad Cheng, Sit Foon Ambati, Ranga Rao Yeong, Hui Yin Phang, Siew Moi Q Science (General) QD Chemistry QH Natural history In this study, Gelidium elegans is investigated for ethanol production. A combination of factors including different temperatures, acid concentration and incubation time was evaluated to determine the suitable saccharification conditions. The combination of 2.5% (w/v) H2SO4 at 120 °C for 40 min was selected for hydrolysis of the seaweed biomass, followed by purification, and fermentation to yield ethanol. The galactose and glucose were dominant reducing sugars in the G. elegans hydrolysate and under optimum condition of dilute acid hydrolysis, 39.42% of reducing sugars was produced and fermentation resulted in ethanol concentration of 13.27 ± 0.47 g/L. A modified method was evaluated for sample preparation for gas chromatography (GC) analysis of the ethanol content. A solvent mixture of acetonitrile and iso-butanol precipitated dissolved organic residues and reduced water content in GC samples at least by 90%. Results showed that this method could be successfully used for bioethanol production from seaweed. Springer Verlag (Germany) 2019 Article PeerReviewed Hessami, Mohammad Javad and Cheng, Sit Foon and Ambati, Ranga Rao and Yeong, Hui Yin and Phang, Siew Moi (2019) Bioethanol production from agarophyte red seaweed, Gelidium elegans, using a novel sample preparation method for analysing bioethanol content by gas chromatography. 3 Biotech, 9 (1). p. 25. ISSN 2190-572X, DOI https://doi.org/10.1007/s13205-018-1549-8 <https://doi.org/10.1007/s13205-018-1549-8>. https://doi.org/10.1007/s13205-018-1549-8 doi:10.1007/s13205-018-1549-8 |
| spellingShingle | Q Science (General) QD Chemistry QH Natural history Hessami, Mohammad Javad Cheng, Sit Foon Ambati, Ranga Rao Yeong, Hui Yin Phang, Siew Moi Bioethanol production from agarophyte red seaweed, Gelidium elegans, using a novel sample preparation method for analysing bioethanol content by gas chromatography |
| title | Bioethanol production from agarophyte red seaweed, Gelidium elegans, using a novel sample preparation method for analysing bioethanol content by gas chromatography |
| title_full | Bioethanol production from agarophyte red seaweed, Gelidium elegans, using a novel sample preparation method for analysing bioethanol content by gas chromatography |
| title_fullStr | Bioethanol production from agarophyte red seaweed, Gelidium elegans, using a novel sample preparation method for analysing bioethanol content by gas chromatography |
| title_full_unstemmed | Bioethanol production from agarophyte red seaweed, Gelidium elegans, using a novel sample preparation method for analysing bioethanol content by gas chromatography |
| title_short | Bioethanol production from agarophyte red seaweed, Gelidium elegans, using a novel sample preparation method for analysing bioethanol content by gas chromatography |
| title_sort | bioethanol production from agarophyte red seaweed gelidium elegans using a novel sample preparation method for analysing bioethanol content by gas chromatography |
| topic | Q Science (General) QD Chemistry QH Natural history |
| work_keys_str_mv | AT hessamimohammadjavad bioethanolproductionfromagarophyteredseaweedgelidiumelegansusinganovelsamplepreparationmethodforanalysingbioethanolcontentbygaschromatography AT chengsitfoon bioethanolproductionfromagarophyteredseaweedgelidiumelegansusinganovelsamplepreparationmethodforanalysingbioethanolcontentbygaschromatography AT ambatirangarao bioethanolproductionfromagarophyteredseaweedgelidiumelegansusinganovelsamplepreparationmethodforanalysingbioethanolcontentbygaschromatography AT yeonghuiyin bioethanolproductionfromagarophyteredseaweedgelidiumelegansusinganovelsamplepreparationmethodforanalysingbioethanolcontentbygaschromatography AT phangsiewmoi bioethanolproductionfromagarophyteredseaweedgelidiumelegansusinganovelsamplepreparationmethodforanalysingbioethanolcontentbygaschromatography |