| Summary: | Alexandrium minutum is a saxitoxins-producring marine dinoflagellate that responsible for paralytic shellfish
poisoning (PSP). It is widely distributed in the tropical and temperate waters with various hydrographic regimes. The
physiological responses of this species to various macronutrients have been studied intensively, but little is known about the regulating mechanism of nutrient uptakes and their roles in saxitoxin production. The effect of varying levels of nitrogen and phosphorous nutrition on the transcriptional responses of a tropical ribotype of A. minutum was investigated in a laboratory setting. The nutrient-related genes, ammonium transporter (AmAmt1), nitrate transporter (AmNrt2), phosphate transporter (AmPiPT1), nitrate reductase (AmNas), glutamine synthetase (AmGSIII) and carbarmoyl phosphate synthase (AmCPSIII) were assembled from Sequence Read Archive (SRA) data set, and later used in the differential gene expression analyzed by qPCR. The results showed that, AmAmt1 was suppressed in excess ammonium-grown culture; conversely AmNrt2 and AmNas were induced. Gene expression of AmAmt1, AmNrt2, AmNas, AmGSIII, AmCPSII and AmPiPT1 was highly induced under P-deficient condition, suggesting that the cells are
scavenging to take up nutrients in the P-stress condition. Gene expression of AmCPSII was well correlated with the
toxin cell quota, suggesting the gene might involve in arginine metabolism and simultaneously increased the toxin
production. Nonetheless, AmGSIII expression might play a role in the stress adaption towards high toxic levels of
ammonium ambient by producing toxins as a counteracting response. The results of this study have provided better
insights into the eco-physiology of A. minutum in relation to the toxin production and its adaptive strategies in
unfavorable environments.
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