The role of nutrient-related genes in understanding the saxitoxin production of Alexandrium minutum

Alexandrium minutum is a saxitoxins-producring marine dinoflagellate that responsible for paralytic shellfish poisoning (PSP). It is widely distributed in the tropical and temperate waters with various hydrographic regimes. The physiological responses of this species to various macronutrients have...

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Main Authors: Hii, Kieng Soon, Kon, N.F., Lim, P.T., Leaw, Chui Pin
Format: Conference or Workshop Item
Language:English
Published: 2018
Subjects:
Online Access:http://eprints.um.edu.my/20256/1/Pages%20from%20ICHA%202018_Abstract%20Book-2.pdf
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author Hii, Kieng Soon
Kon, N.F.
Lim, P.T.
Leaw, Chui Pin
author_facet Hii, Kieng Soon
Kon, N.F.
Lim, P.T.
Leaw, Chui Pin
author_sort Hii, Kieng Soon
collection UM
description Alexandrium minutum is a saxitoxins-producring marine dinoflagellate that responsible for paralytic shellfish poisoning (PSP). It is widely distributed in the tropical and temperate waters with various hydrographic regimes. The physiological responses of this species to various macronutrients have been studied intensively, but little is known about the regulating mechanism of nutrient uptakes and their roles in saxitoxin production. The effect of varying levels of nitrogen and phosphorous nutrition on the transcriptional responses of a tropical ribotype of A. minutum was investigated in a laboratory setting. The nutrient-related genes, ammonium transporter (AmAmt1), nitrate transporter (AmNrt2), phosphate transporter (AmPiPT1), nitrate reductase (AmNas), glutamine synthetase (AmGSIII) and carbarmoyl phosphate synthase (AmCPSIII) were assembled from Sequence Read Archive (SRA) data set, and later used in the differential gene expression analyzed by qPCR. The results showed that, AmAmt1 was suppressed in excess ammonium-grown culture; conversely AmNrt2 and AmNas were induced. Gene expression of AmAmt1, AmNrt2, AmNas, AmGSIII, AmCPSII and AmPiPT1 was highly induced under P-deficient condition, suggesting that the cells are scavenging to take up nutrients in the P-stress condition. Gene expression of AmCPSII was well correlated with the toxin cell quota, suggesting the gene might involve in arginine metabolism and simultaneously increased the toxin production. Nonetheless, AmGSIII expression might play a role in the stress adaption towards high toxic levels of ammonium ambient by producing toxins as a counteracting response. The results of this study have provided better insights into the eco-physiology of A. minutum in relation to the toxin production and its adaptive strategies in unfavorable environments.
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spelling um.eprints-202562019-02-08T03:34:17Z http://eprints.um.edu.my/20256/ The role of nutrient-related genes in understanding the saxitoxin production of Alexandrium minutum Hii, Kieng Soon Kon, N.F. Lim, P.T. Leaw, Chui Pin Q Science (General) QH301 Biology Alexandrium minutum is a saxitoxins-producring marine dinoflagellate that responsible for paralytic shellfish poisoning (PSP). It is widely distributed in the tropical and temperate waters with various hydrographic regimes. The physiological responses of this species to various macronutrients have been studied intensively, but little is known about the regulating mechanism of nutrient uptakes and their roles in saxitoxin production. The effect of varying levels of nitrogen and phosphorous nutrition on the transcriptional responses of a tropical ribotype of A. minutum was investigated in a laboratory setting. The nutrient-related genes, ammonium transporter (AmAmt1), nitrate transporter (AmNrt2), phosphate transporter (AmPiPT1), nitrate reductase (AmNas), glutamine synthetase (AmGSIII) and carbarmoyl phosphate synthase (AmCPSIII) were assembled from Sequence Read Archive (SRA) data set, and later used in the differential gene expression analyzed by qPCR. The results showed that, AmAmt1 was suppressed in excess ammonium-grown culture; conversely AmNrt2 and AmNas were induced. Gene expression of AmAmt1, AmNrt2, AmNas, AmGSIII, AmCPSII and AmPiPT1 was highly induced under P-deficient condition, suggesting that the cells are scavenging to take up nutrients in the P-stress condition. Gene expression of AmCPSII was well correlated with the toxin cell quota, suggesting the gene might involve in arginine metabolism and simultaneously increased the toxin production. Nonetheless, AmGSIII expression might play a role in the stress adaption towards high toxic levels of ammonium ambient by producing toxins as a counteracting response. The results of this study have provided better insights into the eco-physiology of A. minutum in relation to the toxin production and its adaptive strategies in unfavorable environments. 2018 Conference or Workshop Item PeerReviewed text en http://eprints.um.edu.my/20256/1/Pages%20from%20ICHA%202018_Abstract%20Book-2.pdf Hii, Kieng Soon and Kon, N.F. and Lim, P.T. and Leaw, Chui Pin (2018) The role of nutrient-related genes in understanding the saxitoxin production of Alexandrium minutum. In: 18th International Conference on Harmful Algae (ICHA 2018), 21-26 October 2018, Nantes, France. https://www.icha2018.com/medias/content/files/ICHA_2018_Abstract_Book_v7.pdf
spellingShingle Q Science (General)
QH301 Biology
Hii, Kieng Soon
Kon, N.F.
Lim, P.T.
Leaw, Chui Pin
The role of nutrient-related genes in understanding the saxitoxin production of Alexandrium minutum
title The role of nutrient-related genes in understanding the saxitoxin production of Alexandrium minutum
title_full The role of nutrient-related genes in understanding the saxitoxin production of Alexandrium minutum
title_fullStr The role of nutrient-related genes in understanding the saxitoxin production of Alexandrium minutum
title_full_unstemmed The role of nutrient-related genes in understanding the saxitoxin production of Alexandrium minutum
title_short The role of nutrient-related genes in understanding the saxitoxin production of Alexandrium minutum
title_sort role of nutrient related genes in understanding the saxitoxin production of alexandrium minutum
topic Q Science (General)
QH301 Biology
url http://eprints.um.edu.my/20256/1/Pages%20from%20ICHA%202018_Abstract%20Book-2.pdf
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