Fucosterol inhibits the cholinesterase activities and reduces the release of pro-inflammatory mediators in lipopolysaccharide and amyloid-induced microglial cells
According to the cholinergic hypothesis, memory impairment in patients with Alzheimer’s disease (AD) is associated with the deficit of cholinergic function in the brain. In addition, microglial activation plays an important role in AD by producing pro-inflammatory cytokines, nitric oxide (NO), and p...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Springer Verlag
2018
|
| Subjects: |
| _version_ | 1796960868299177984 |
|---|---|
| author | Wong, Chun Hoong Gan, Sook Yee Tan, Swee Ching Gany, Siti Aisya Ying, Tiong Gray, Alexander Irvine Igoli, John Chan, Elaine Wan Ling Phang, Siew Moi |
| author_facet | Wong, Chun Hoong Gan, Sook Yee Tan, Swee Ching Gany, Siti Aisya Ying, Tiong Gray, Alexander Irvine Igoli, John Chan, Elaine Wan Ling Phang, Siew Moi |
| author_sort | Wong, Chun Hoong |
| collection | UM |
| description | According to the cholinergic hypothesis, memory impairment in patients with Alzheimer’s disease (AD) is associated with the deficit of cholinergic function in the brain. In addition, microglial activation plays an important role in AD by producing pro-inflammatory cytokines, nitric oxide (NO), and prostaglandin E2 (PGE2). It was noted that lipopolysaccharide (LPS) and β-amyloid (Aβ) induced microglial activation leading to neuroinflammation and ultimately neuronal cell death. Fucosterol, a plant sterol found in brown algae, has been reported to exhibit several bioactivities. This study aimed to investigate the anti-cholinesterase activities of fucosterol and its effects on the release of pro-inflammatory mediators by LPS- and Aβ-induced microglial cells. Cholinesterase inhibition was determined using the modified Ellman colorimetric method. Expression of pro-inflammatory mediators was determined using RT-PCR and ELISA. The NO content was determined using the Griess test. Fucosterol exhibited dose-dependent inhibitory activities against both acetylcholinesterase and butyrylcholinesterase. It significantly inhibited the production of cytokines, namely interleukins (IL-6, IL-1β), tumor necrosis factor-α (TNF-α), NO, and PGE2 in LPS- or Aβ-induced microglial cells. Fucosterol provided protective effects against Aβ-mediated neuroinflammation by inhibiting the production of pro-inflammatory mediators. These findings provided insights into the development of fucosterol as a potential drug candidate for AD, a multifactorial neurodegenerative disorder. |
| first_indexed | 2024-03-06T05:51:01Z |
| format | Article |
| id | um.eprints-20373 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:51:01Z |
| publishDate | 2018 |
| publisher | Springer Verlag |
| record_format | dspace |
| spelling | um.eprints-203732019-02-18T05:36:45Z http://eprints.um.edu.my/20373/ Fucosterol inhibits the cholinesterase activities and reduces the release of pro-inflammatory mediators in lipopolysaccharide and amyloid-induced microglial cells Wong, Chun Hoong Gan, Sook Yee Tan, Swee Ching Gany, Siti Aisya Ying, Tiong Gray, Alexander Irvine Igoli, John Chan, Elaine Wan Ling Phang, Siew Moi Q Science (General) QH Natural history According to the cholinergic hypothesis, memory impairment in patients with Alzheimer’s disease (AD) is associated with the deficit of cholinergic function in the brain. In addition, microglial activation plays an important role in AD by producing pro-inflammatory cytokines, nitric oxide (NO), and prostaglandin E2 (PGE2). It was noted that lipopolysaccharide (LPS) and β-amyloid (Aβ) induced microglial activation leading to neuroinflammation and ultimately neuronal cell death. Fucosterol, a plant sterol found in brown algae, has been reported to exhibit several bioactivities. This study aimed to investigate the anti-cholinesterase activities of fucosterol and its effects on the release of pro-inflammatory mediators by LPS- and Aβ-induced microglial cells. Cholinesterase inhibition was determined using the modified Ellman colorimetric method. Expression of pro-inflammatory mediators was determined using RT-PCR and ELISA. The NO content was determined using the Griess test. Fucosterol exhibited dose-dependent inhibitory activities against both acetylcholinesterase and butyrylcholinesterase. It significantly inhibited the production of cytokines, namely interleukins (IL-6, IL-1β), tumor necrosis factor-α (TNF-α), NO, and PGE2 in LPS- or Aβ-induced microglial cells. Fucosterol provided protective effects against Aβ-mediated neuroinflammation by inhibiting the production of pro-inflammatory mediators. These findings provided insights into the development of fucosterol as a potential drug candidate for AD, a multifactorial neurodegenerative disorder. Springer Verlag 2018 Article PeerReviewed Wong, Chun Hoong and Gan, Sook Yee and Tan, Swee Ching and Gany, Siti Aisya and Ying, Tiong and Gray, Alexander Irvine and Igoli, John and Chan, Elaine Wan Ling and Phang, Siew Moi (2018) Fucosterol inhibits the cholinesterase activities and reduces the release of pro-inflammatory mediators in lipopolysaccharide and amyloid-induced microglial cells. Journal of Applied Phycology, 30 (6). pp. 3261-3270. ISSN 0921-8971, DOI https://doi.org/10.1007/s10811-018-1495-1 <https://doi.org/10.1007/s10811-018-1495-1>. https://doi.org/10.1007/s10811-018-1495-1 doi:10.1007/s10811-018-1495-1 |
| spellingShingle | Q Science (General) QH Natural history Wong, Chun Hoong Gan, Sook Yee Tan, Swee Ching Gany, Siti Aisya Ying, Tiong Gray, Alexander Irvine Igoli, John Chan, Elaine Wan Ling Phang, Siew Moi Fucosterol inhibits the cholinesterase activities and reduces the release of pro-inflammatory mediators in lipopolysaccharide and amyloid-induced microglial cells |
| title | Fucosterol inhibits the cholinesterase activities and reduces the release of pro-inflammatory mediators in lipopolysaccharide and amyloid-induced microglial cells |
| title_full | Fucosterol inhibits the cholinesterase activities and reduces the release of pro-inflammatory mediators in lipopolysaccharide and amyloid-induced microglial cells |
| title_fullStr | Fucosterol inhibits the cholinesterase activities and reduces the release of pro-inflammatory mediators in lipopolysaccharide and amyloid-induced microglial cells |
| title_full_unstemmed | Fucosterol inhibits the cholinesterase activities and reduces the release of pro-inflammatory mediators in lipopolysaccharide and amyloid-induced microglial cells |
| title_short | Fucosterol inhibits the cholinesterase activities and reduces the release of pro-inflammatory mediators in lipopolysaccharide and amyloid-induced microglial cells |
| title_sort | fucosterol inhibits the cholinesterase activities and reduces the release of pro inflammatory mediators in lipopolysaccharide and amyloid induced microglial cells |
| topic | Q Science (General) QH Natural history |
| work_keys_str_mv | AT wongchunhoong fucosterolinhibitsthecholinesteraseactivitiesandreducesthereleaseofproinflammatorymediatorsinlipopolysaccharideandamyloidinducedmicroglialcells AT gansookyee fucosterolinhibitsthecholinesteraseactivitiesandreducesthereleaseofproinflammatorymediatorsinlipopolysaccharideandamyloidinducedmicroglialcells AT tansweeching fucosterolinhibitsthecholinesteraseactivitiesandreducesthereleaseofproinflammatorymediatorsinlipopolysaccharideandamyloidinducedmicroglialcells AT ganysitiaisya fucosterolinhibitsthecholinesteraseactivitiesandreducesthereleaseofproinflammatorymediatorsinlipopolysaccharideandamyloidinducedmicroglialcells AT yingtiong fucosterolinhibitsthecholinesteraseactivitiesandreducesthereleaseofproinflammatorymediatorsinlipopolysaccharideandamyloidinducedmicroglialcells AT grayalexanderirvine fucosterolinhibitsthecholinesteraseactivitiesandreducesthereleaseofproinflammatorymediatorsinlipopolysaccharideandamyloidinducedmicroglialcells AT igolijohn fucosterolinhibitsthecholinesteraseactivitiesandreducesthereleaseofproinflammatorymediatorsinlipopolysaccharideandamyloidinducedmicroglialcells AT chanelainewanling fucosterolinhibitsthecholinesteraseactivitiesandreducesthereleaseofproinflammatorymediatorsinlipopolysaccharideandamyloidinducedmicroglialcells AT phangsiewmoi fucosterolinhibitsthecholinesteraseactivitiesandreducesthereleaseofproinflammatorymediatorsinlipopolysaccharideandamyloidinducedmicroglialcells |