Screening and characterisation of two strains of Pseudomonas aeruginosa from aquaculture and water environment
Aims: Pseudomonas has been associated with diseases occurring in people with weakened or compromised immune system after exposure to contaminated water. The diseases are commonly treated with antibiotics. However, the bacteria had developed resistances to commonly used antibiotics making treatment a...
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Malaysian Society for Microbiology
2018
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| author | Toh, Seng Chiew Lihan, Samuel Soh, Khar Mun Uyub, Natalia Chai, Lay Ching Müller, Moritz |
| author_facet | Toh, Seng Chiew Lihan, Samuel Soh, Khar Mun Uyub, Natalia Chai, Lay Ching Müller, Moritz |
| author_sort | Toh, Seng Chiew |
| collection | UM |
| description | Aims: Pseudomonas has been associated with diseases occurring in people with weakened or compromised immune system after exposure to contaminated water. The diseases are commonly treated with antibiotics. However, the bacteria had developed resistances to commonly used antibiotics making treatment a difficult task. Therefore, the continuous surveillance of susceptibility of Pseudomonas especially for the human pathogen P. aeruginosa to commonly clinical and aquaculture farming used antibiotics is important to ensure that serious infections remain susceptible to those antibiotics. Methodology and results: In this study, the bacteria were screened from water, sediment and fish from rivers and aquaculture farms around Kuching, Sarawak. A total number of 38 presumptive P. aeruginosa were isolated using CHROMagarTM Pseudomonas and subjected to a series of biochemical tests. Out of all the isolates tested, only two isolates designated as AS-R10(S) and BK2-OLT2(S) fulfilled the biochemical characteristics of P. aeruginosa. 16S rRNA gene sequencing further confirmed these two isolates as P. aeruginosa based on their 100% similarity with P. aeruginosa strain GD1 and P. aeruginosa strain PA1201 in NCBI database. These two isolates were tested for their susceptibilities against nine common antibiotics used in both clinical and aquaculture farming nowadays: imipenem, piperacillin, meropenem, amikacin, gentamicin, ciprofloxacin, ceftazidime, tobramycin and norfloxacin according to CLSI standard using disk diffusion method. Conclusion, significance and impact of study: The two isolates exhibited total susceptibility to all the antibiotics analysed, suggesting the effectiveness of the antimicrobial agents towards P. aeruginosa isolated from aquaculture and water environment in the study area. |
| first_indexed | 2024-03-06T05:51:16Z |
| format | Article |
| id | um.eprints-20460 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:51:16Z |
| publishDate | 2018 |
| publisher | Malaysian Society for Microbiology |
| record_format | dspace |
| spelling | um.eprints-204602019-02-25T02:16:52Z http://eprints.um.edu.my/20460/ Screening and characterisation of two strains of Pseudomonas aeruginosa from aquaculture and water environment Toh, Seng Chiew Lihan, Samuel Soh, Khar Mun Uyub, Natalia Chai, Lay Ching Müller, Moritz Q Science (General) QH Natural history Aims: Pseudomonas has been associated with diseases occurring in people with weakened or compromised immune system after exposure to contaminated water. The diseases are commonly treated with antibiotics. However, the bacteria had developed resistances to commonly used antibiotics making treatment a difficult task. Therefore, the continuous surveillance of susceptibility of Pseudomonas especially for the human pathogen P. aeruginosa to commonly clinical and aquaculture farming used antibiotics is important to ensure that serious infections remain susceptible to those antibiotics. Methodology and results: In this study, the bacteria were screened from water, sediment and fish from rivers and aquaculture farms around Kuching, Sarawak. A total number of 38 presumptive P. aeruginosa were isolated using CHROMagarTM Pseudomonas and subjected to a series of biochemical tests. Out of all the isolates tested, only two isolates designated as AS-R10(S) and BK2-OLT2(S) fulfilled the biochemical characteristics of P. aeruginosa. 16S rRNA gene sequencing further confirmed these two isolates as P. aeruginosa based on their 100% similarity with P. aeruginosa strain GD1 and P. aeruginosa strain PA1201 in NCBI database. These two isolates were tested for their susceptibilities against nine common antibiotics used in both clinical and aquaculture farming nowadays: imipenem, piperacillin, meropenem, amikacin, gentamicin, ciprofloxacin, ceftazidime, tobramycin and norfloxacin according to CLSI standard using disk diffusion method. Conclusion, significance and impact of study: The two isolates exhibited total susceptibility to all the antibiotics analysed, suggesting the effectiveness of the antimicrobial agents towards P. aeruginosa isolated from aquaculture and water environment in the study area. Malaysian Society for Microbiology 2018 Article PeerReviewed Toh, Seng Chiew and Lihan, Samuel and Soh, Khar Mun and Uyub, Natalia and Chai, Lay Ching and Müller, Moritz (2018) Screening and characterisation of two strains of Pseudomonas aeruginosa from aquaculture and water environment. Malaysian Journal of Microbiology, 14 (4). pp. 329-334. ISSN 1823-8262, DOI https://doi.org/10.21161/mjm.144186 <https://doi.org/10.21161/mjm.144186>. http://mjm.usm.my/uploads/issues/1422/MJM-144186.pdf doi:10.21161/mjm.144186 |
| spellingShingle | Q Science (General) QH Natural history Toh, Seng Chiew Lihan, Samuel Soh, Khar Mun Uyub, Natalia Chai, Lay Ching Müller, Moritz Screening and characterisation of two strains of Pseudomonas aeruginosa from aquaculture and water environment |
| title | Screening and characterisation of two strains of Pseudomonas aeruginosa from aquaculture and water environment |
| title_full | Screening and characterisation of two strains of Pseudomonas aeruginosa from aquaculture and water environment |
| title_fullStr | Screening and characterisation of two strains of Pseudomonas aeruginosa from aquaculture and water environment |
| title_full_unstemmed | Screening and characterisation of two strains of Pseudomonas aeruginosa from aquaculture and water environment |
| title_short | Screening and characterisation of two strains of Pseudomonas aeruginosa from aquaculture and water environment |
| title_sort | screening and characterisation of two strains of pseudomonas aeruginosa from aquaculture and water environment |
| topic | Q Science (General) QH Natural history |
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