Prevalence, Virulence Genes and Antimicrobial Resistance Profiles of Salmonella Serovars from Retail Beef in Selangor, Malaysia
The aim of the present study was to investigate the prevalence of Salmonella spp., Salmonella Enteritidis and Salmonella Typhimurium in retail beef from different retail markets of Selangor area, as well as, to assess their pathogenic potential and antimicrobial resistance. A total of 240 retail bee...
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Frontiers Media
2018
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| author | Thung, Tze Y. Radu, Son Mahyudin, Nor A. Rukayadi, Yaya Zakaria, Zunita Mazlan, Nurzafirah Tan, Boon H. Lee, Epeng Yeoh, Soo L. Chin, Yih Z. Tan, Chia W. Kuan, Chee H. Basri, Dayang F. Wan Mohamed Radzi, Che Wan Jasimah |
| author_facet | Thung, Tze Y. Radu, Son Mahyudin, Nor A. Rukayadi, Yaya Zakaria, Zunita Mazlan, Nurzafirah Tan, Boon H. Lee, Epeng Yeoh, Soo L. Chin, Yih Z. Tan, Chia W. Kuan, Chee H. Basri, Dayang F. Wan Mohamed Radzi, Che Wan Jasimah |
| author_sort | Thung, Tze Y. |
| collection | UM |
| description | The aim of the present study was to investigate the prevalence of Salmonella spp., Salmonella Enteritidis and Salmonella Typhimurium in retail beef from different retail markets of Selangor area, as well as, to assess their pathogenic potential and antimicrobial resistance. A total of 240 retail beef meat samples (chuck = 60; rib = 60; round = 60; sirloin = 60) were randomly collected. The multiplex polymerase chain reaction (mPCR) in combination with the most probable number (MPN) method was employed to detect Salmonella spp., S. Enteritidis and S. Typhimurium in the meat samples. The prevalence of Salmonella spp., S. Enteritidis and S. Typhimurium in 240 beef meat samples were 7.50, 1.25, and 0.83%, respectively. The microbial loads of total Salmonella was found in the range of < 3 to 15 MPN/g. Eight different serovars of Salmonella were identified among the 23 isolates, and S. Agona was the predominant serovar (26.09%). Interestingly, all the Salmonella isolates were resistant to penicillin, erythromycin and vancomycin, but the sensitivity was observed for tetracycline, gentamicin and amoxicillin/clavulanic acid. All 23 isolates were resistant to at least three antibiotics. Two S. Typhimurium isolates (8.70%) exhibited the highest multiple antibiotic resistance (MAR) index value of 0.56 which shown resistance to nine antibiotics. PCR analysis of virulence genes showed that all Salmonella isolates (100%) were positive for the invA gene. Meanwhile, pefA was only identified in S. Enteritidis and S. Typhimurium. The findings in this study indicate that retail beef products tested were widely contaminated with multi-drug resistant (MDR) Salmonella and various virulence genes are present among the isolated Salmonella serovars. |
| first_indexed | 2024-03-06T05:51:18Z |
| format | Article |
| id | um.eprints-20471 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:51:18Z |
| publishDate | 2018 |
| publisher | Frontiers Media |
| record_format | dspace |
| spelling | um.eprints-204712019-02-25T03:51:22Z http://eprints.um.edu.my/20471/ Prevalence, Virulence Genes and Antimicrobial Resistance Profiles of Salmonella Serovars from Retail Beef in Selangor, Malaysia Thung, Tze Y. Radu, Son Mahyudin, Nor A. Rukayadi, Yaya Zakaria, Zunita Mazlan, Nurzafirah Tan, Boon H. Lee, Epeng Yeoh, Soo L. Chin, Yih Z. Tan, Chia W. Kuan, Chee H. Basri, Dayang F. Wan Mohamed Radzi, Che Wan Jasimah Q Science (General) QH Natural history T Technology (General) The aim of the present study was to investigate the prevalence of Salmonella spp., Salmonella Enteritidis and Salmonella Typhimurium in retail beef from different retail markets of Selangor area, as well as, to assess their pathogenic potential and antimicrobial resistance. A total of 240 retail beef meat samples (chuck = 60; rib = 60; round = 60; sirloin = 60) were randomly collected. The multiplex polymerase chain reaction (mPCR) in combination with the most probable number (MPN) method was employed to detect Salmonella spp., S. Enteritidis and S. Typhimurium in the meat samples. The prevalence of Salmonella spp., S. Enteritidis and S. Typhimurium in 240 beef meat samples were 7.50, 1.25, and 0.83%, respectively. The microbial loads of total Salmonella was found in the range of < 3 to 15 MPN/g. Eight different serovars of Salmonella were identified among the 23 isolates, and S. Agona was the predominant serovar (26.09%). Interestingly, all the Salmonella isolates were resistant to penicillin, erythromycin and vancomycin, but the sensitivity was observed for tetracycline, gentamicin and amoxicillin/clavulanic acid. All 23 isolates were resistant to at least three antibiotics. Two S. Typhimurium isolates (8.70%) exhibited the highest multiple antibiotic resistance (MAR) index value of 0.56 which shown resistance to nine antibiotics. PCR analysis of virulence genes showed that all Salmonella isolates (100%) were positive for the invA gene. Meanwhile, pefA was only identified in S. Enteritidis and S. Typhimurium. The findings in this study indicate that retail beef products tested were widely contaminated with multi-drug resistant (MDR) Salmonella and various virulence genes are present among the isolated Salmonella serovars. Frontiers Media 2018 Article PeerReviewed Thung, Tze Y. and Radu, Son and Mahyudin, Nor A. and Rukayadi, Yaya and Zakaria, Zunita and Mazlan, Nurzafirah and Tan, Boon H. and Lee, Epeng and Yeoh, Soo L. and Chin, Yih Z. and Tan, Chia W. and Kuan, Chee H. and Basri, Dayang F. and Wan Mohamed Radzi, Che Wan Jasimah (2018) Prevalence, Virulence Genes and Antimicrobial Resistance Profiles of Salmonella Serovars from Retail Beef in Selangor, Malaysia. Frontiers in Microbiology, 8. p. 2697. ISSN 1664-302X, DOI https://doi.org/10.3389/fmicb.2017.02697 <https://doi.org/10.3389/fmicb.2017.02697>. https://doi.org/10.3389/fmicb.2017.02697 doi:10.3389/fmicb.2017.02697 |
| spellingShingle | Q Science (General) QH Natural history T Technology (General) Thung, Tze Y. Radu, Son Mahyudin, Nor A. Rukayadi, Yaya Zakaria, Zunita Mazlan, Nurzafirah Tan, Boon H. Lee, Epeng Yeoh, Soo L. Chin, Yih Z. Tan, Chia W. Kuan, Chee H. Basri, Dayang F. Wan Mohamed Radzi, Che Wan Jasimah Prevalence, Virulence Genes and Antimicrobial Resistance Profiles of Salmonella Serovars from Retail Beef in Selangor, Malaysia |
| title | Prevalence, Virulence Genes and Antimicrobial Resistance Profiles of Salmonella Serovars from Retail Beef in Selangor, Malaysia |
| title_full | Prevalence, Virulence Genes and Antimicrobial Resistance Profiles of Salmonella Serovars from Retail Beef in Selangor, Malaysia |
| title_fullStr | Prevalence, Virulence Genes and Antimicrobial Resistance Profiles of Salmonella Serovars from Retail Beef in Selangor, Malaysia |
| title_full_unstemmed | Prevalence, Virulence Genes and Antimicrobial Resistance Profiles of Salmonella Serovars from Retail Beef in Selangor, Malaysia |
| title_short | Prevalence, Virulence Genes and Antimicrobial Resistance Profiles of Salmonella Serovars from Retail Beef in Selangor, Malaysia |
| title_sort | prevalence virulence genes and antimicrobial resistance profiles of salmonella serovars from retail beef in selangor malaysia |
| topic | Q Science (General) QH Natural history T Technology (General) |
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