A recombinant subunit HS aba392 as a potential vaccine for haemorrhagic septicaemia disease in livestock
Haemorrhagic septicaemia (HS) is a major disease in cattle and buffaloes, caused by certain serotypes of Pasteurella multocida, mainly B and E serotypes. Frequent HS outbreak has a major impact in many Asian countries, including Malaysia, where farmers encounter economic loss due to low milk product...
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Malaysian Society of Parasitology and Tropical Medicine
2018
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| author | Rita, D.V. Swee, K.C.W. Shamini, C. Kang, Tzin Lim Nurshamimi, N.R. Hussin, A.R. Ismail, Salmah |
| author_facet | Rita, D.V. Swee, K.C.W. Shamini, C. Kang, Tzin Lim Nurshamimi, N.R. Hussin, A.R. Ismail, Salmah |
| author_sort | Rita, D.V. |
| collection | UM |
| description | Haemorrhagic septicaemia (HS) is a major disease in cattle and buffaloes, caused by certain serotypes of Pasteurella multocida, mainly B and E serotypes. Frequent HS outbreak has a major impact in many Asian countries, including Malaysia, where farmers encounter economic loss due to low milk production as well as death of their livestock. There are four types of vaccines available; broth bacterins, alum precipitated vaccine, aluminium hydroxide gel vaccine and oil adjuvant vaccine (OAV), but these vaccines can only provide short term immunity and therefore need to be administered annually. Hence, the development of a protein vaccine using recombinant antigen can be a potential candidate for the production of HS vaccine that would give longer immunity. We have successfully cloned the ABA392 gene fragment into a protein expression vector, pET-30a. The protein was expressed from our ABA392/pET30a clone and the immunogenicity of the protein has been tested on rats. This vaccine was able to trigger an immune response and therefore has the potential to be tested as suitable vaccine candidate in future studies. It is envisaged that this subunit vaccine will make a significant contribution in the management of HS among livestock in future. |
| first_indexed | 2024-03-06T05:52:34Z |
| format | Article |
| id | um.eprints-20883 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:52:34Z |
| publishDate | 2018 |
| publisher | Malaysian Society of Parasitology and Tropical Medicine |
| record_format | dspace |
| spelling | um.eprints-208832019-04-10T06:55:06Z http://eprints.um.edu.my/20883/ A recombinant subunit HS aba392 as a potential vaccine for haemorrhagic septicaemia disease in livestock Rita, D.V. Swee, K.C.W. Shamini, C. Kang, Tzin Lim Nurshamimi, N.R. Hussin, A.R. Ismail, Salmah Q Science (General) QH Natural history R Medicine Haemorrhagic septicaemia (HS) is a major disease in cattle and buffaloes, caused by certain serotypes of Pasteurella multocida, mainly B and E serotypes. Frequent HS outbreak has a major impact in many Asian countries, including Malaysia, where farmers encounter economic loss due to low milk production as well as death of their livestock. There are four types of vaccines available; broth bacterins, alum precipitated vaccine, aluminium hydroxide gel vaccine and oil adjuvant vaccine (OAV), but these vaccines can only provide short term immunity and therefore need to be administered annually. Hence, the development of a protein vaccine using recombinant antigen can be a potential candidate for the production of HS vaccine that would give longer immunity. We have successfully cloned the ABA392 gene fragment into a protein expression vector, pET-30a. The protein was expressed from our ABA392/pET30a clone and the immunogenicity of the protein has been tested on rats. This vaccine was able to trigger an immune response and therefore has the potential to be tested as suitable vaccine candidate in future studies. It is envisaged that this subunit vaccine will make a significant contribution in the management of HS among livestock in future. Malaysian Society of Parasitology and Tropical Medicine 2018 Article PeerReviewed Rita, D.V. and Swee, K.C.W. and Shamini, C. and Kang, Tzin Lim and Nurshamimi, N.R. and Hussin, A.R. and Ismail, Salmah (2018) A recombinant subunit HS aba392 as a potential vaccine for haemorrhagic septicaemia disease in livestock. Tropical Biomedicine, 35 (4). pp. 1075-1086. ISSN 0127-5720, http://msptm.org/files/Vol35No4/1075-1086-Salmah-I.pdf |
| spellingShingle | Q Science (General) QH Natural history R Medicine Rita, D.V. Swee, K.C.W. Shamini, C. Kang, Tzin Lim Nurshamimi, N.R. Hussin, A.R. Ismail, Salmah A recombinant subunit HS aba392 as a potential vaccine for haemorrhagic septicaemia disease in livestock |
| title | A recombinant subunit HS aba392 as a potential vaccine for haemorrhagic septicaemia disease in livestock |
| title_full | A recombinant subunit HS aba392 as a potential vaccine for haemorrhagic septicaemia disease in livestock |
| title_fullStr | A recombinant subunit HS aba392 as a potential vaccine for haemorrhagic septicaemia disease in livestock |
| title_full_unstemmed | A recombinant subunit HS aba392 as a potential vaccine for haemorrhagic septicaemia disease in livestock |
| title_short | A recombinant subunit HS aba392 as a potential vaccine for haemorrhagic septicaemia disease in livestock |
| title_sort | recombinant subunit hs aba392 as a potential vaccine for haemorrhagic septicaemia disease in livestock |
| topic | Q Science (General) QH Natural history R Medicine |
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