Lentinula edodes (shiitake mushroom): An assessment of in vitro anti-atherosclerotic bio-functionality
Mushrooms have been highly regarded as possessing enormous nutritive and medicinal values. In the present study, we evaluated the anti-oxidative and anti-atherosclerotic potential of shiitake mushroom (Lentinula edodes) using its solvent–solvent partitioned fractions that consisted of methanol:dichl...
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| Format: | Article |
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Elsevier
2018
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| _version_ | 1825721600707657728 |
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| author | Rahman, Mohammad Azizur Abdullah, Noorlidah Aminudin, Norhaniza |
| author_facet | Rahman, Mohammad Azizur Abdullah, Noorlidah Aminudin, Norhaniza |
| author_sort | Rahman, Mohammad Azizur |
| collection | UM |
| description | Mushrooms have been highly regarded as possessing enormous nutritive and medicinal values. In the present study, we evaluated the anti-oxidative and anti-atherosclerotic potential of shiitake mushroom (Lentinula edodes) using its solvent–solvent partitioned fractions that consisted of methanol:dichloromethane (M:DCM), hexane (HEX), dichloromethane (DCM), ethyl acetate (EA) and aqueous residue (AQ). The hexane fraction (1 mg/mL) mostly scavenged (67.38%, IC50 0.55 mg/mL) the 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radical, contained the highest reducing capacity (60.16 mg gallic acid equivalents/g fraction), and most potently inhibited lipid peroxidation (67.07%), low density lipo-protein oxidation and the activity of 3-hydroxy 3-methyl glutaryl co-enzyme A reductase (HMGR). GC–MS analyses of the hexane fraction identified α-tocopherol (vitamin E), oleic acid, linoleic acid, ergosterol and butyric acid as the bio-functional components present in L. edodes. Our findings suggest that L. edodes possesses anti-atherosclerotic bio-functionality that can be applied as functional food-based therapeutics against cardiovascular diseases. |
| first_indexed | 2024-03-06T05:52:49Z |
| format | Article |
| id | um.eprints-20980 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:52:49Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | um.eprints-209802019-04-17T09:08:49Z http://eprints.um.edu.my/20980/ Lentinula edodes (shiitake mushroom): An assessment of in vitro anti-atherosclerotic bio-functionality Rahman, Mohammad Azizur Abdullah, Noorlidah Aminudin, Norhaniza Q Science (General) QH Natural history Mushrooms have been highly regarded as possessing enormous nutritive and medicinal values. In the present study, we evaluated the anti-oxidative and anti-atherosclerotic potential of shiitake mushroom (Lentinula edodes) using its solvent–solvent partitioned fractions that consisted of methanol:dichloromethane (M:DCM), hexane (HEX), dichloromethane (DCM), ethyl acetate (EA) and aqueous residue (AQ). The hexane fraction (1 mg/mL) mostly scavenged (67.38%, IC50 0.55 mg/mL) the 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radical, contained the highest reducing capacity (60.16 mg gallic acid equivalents/g fraction), and most potently inhibited lipid peroxidation (67.07%), low density lipo-protein oxidation and the activity of 3-hydroxy 3-methyl glutaryl co-enzyme A reductase (HMGR). GC–MS analyses of the hexane fraction identified α-tocopherol (vitamin E), oleic acid, linoleic acid, ergosterol and butyric acid as the bio-functional components present in L. edodes. Our findings suggest that L. edodes possesses anti-atherosclerotic bio-functionality that can be applied as functional food-based therapeutics against cardiovascular diseases. Elsevier 2018 Article PeerReviewed Rahman, Mohammad Azizur and Abdullah, Noorlidah and Aminudin, Norhaniza (2018) Lentinula edodes (shiitake mushroom): An assessment of in vitro anti-atherosclerotic bio-functionality. Saudi Journal of Biological Sciences, 25 (8). pp. 1515-1523. ISSN 1319-562X, DOI https://doi.org/10.1016/j.sjbs.2016.01.021 <https://doi.org/10.1016/j.sjbs.2016.01.021>. https://doi.org/10.1016/j.sjbs.2016.01.021 doi:10.1016/j.sjbs.2016.01.021 |
| spellingShingle | Q Science (General) QH Natural history Rahman, Mohammad Azizur Abdullah, Noorlidah Aminudin, Norhaniza Lentinula edodes (shiitake mushroom): An assessment of in vitro anti-atherosclerotic bio-functionality |
| title | Lentinula edodes (shiitake mushroom): An assessment of in vitro anti-atherosclerotic bio-functionality |
| title_full | Lentinula edodes (shiitake mushroom): An assessment of in vitro anti-atherosclerotic bio-functionality |
| title_fullStr | Lentinula edodes (shiitake mushroom): An assessment of in vitro anti-atherosclerotic bio-functionality |
| title_full_unstemmed | Lentinula edodes (shiitake mushroom): An assessment of in vitro anti-atherosclerotic bio-functionality |
| title_short | Lentinula edodes (shiitake mushroom): An assessment of in vitro anti-atherosclerotic bio-functionality |
| title_sort | lentinula edodes shiitake mushroom an assessment of in vitro anti atherosclerotic bio functionality |
| topic | Q Science (General) QH Natural history |
| work_keys_str_mv | AT rahmanmohammadazizur lentinulaedodesshiitakemushroomanassessmentofinvitroantiatheroscleroticbiofunctionality AT abdullahnoorlidah lentinulaedodesshiitakemushroomanassessmentofinvitroantiatheroscleroticbiofunctionality AT aminudinnorhaniza lentinulaedodesshiitakemushroomanassessmentofinvitroantiatheroscleroticbiofunctionality |