Neuritogenic and in vitro antioxidant activities of Malaysian Gracilaria manilaensis Yamamoto & Trono
Numerous marine-based compounds have been identified as having diverse biological activities. Gracilaria manilaensis Yamamoto & Trono is a red agarophyte endemic to the Malaysia coastal area. It is used as a gelling and thickening agent for food industries. In this study, the rat pheochromocytom...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
Springer Verlag
2018
|
| Subjects: |
| _version_ | 1796961354666475520 |
|---|---|
| author | Pang, Jun Rui Goh, Veronica Ming Jun Tan, Cheng Yau Phang, Siew Moi Wong, Kah Hui Yow, Yoon Yen |
| author_facet | Pang, Jun Rui Goh, Veronica Ming Jun Tan, Cheng Yau Phang, Siew Moi Wong, Kah Hui Yow, Yoon Yen |
| author_sort | Pang, Jun Rui |
| collection | UM |
| description | Numerous marine-based compounds have been identified as having diverse biological activities. Gracilaria manilaensis Yamamoto & Trono is a red agarophyte endemic to the Malaysia coastal area. It is used as a gelling and thickening agent for food industries. In this study, the rat pheochromocytoma cell line (PC-12Adh) was employed as in vitro model to investigate the neuritogenic activities in hot aqueous, methanol, and ethanol extracts of G. manilaensis. Phytochemical contents and in vitro antioxidant activities of the extracts were also determined. All G. manilaensis extracts were found to increase the percentage of neurite-bearing cells and upregulated the neuronal cytoskeleton of PC-12Adh cells with no inhibition across the tested concentration range (0–0.15 mg mL −1 ). The methanol and ethanol extracts had higher phenolic and flavonoid contents than the hot aqueous extract. They also showed higher antioxidant activities against 2,2-diphenyl-1-pircryhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) free radicals and higher reducing power compared to the hot aqueous extract. It is believed that G. manilaensis may possess potent bioactive compounds that mimic the neuroactivity of the nerve growth factor (NGF) for neuronal survival, development, and differentiation. It has been suggested that agar producer G. manilaensis might be a promising dietary supplement for cognitive health in the prevention of neurological disorders. |
| first_indexed | 2024-03-06T05:53:00Z |
| format | Article |
| id | um.eprints-21041 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:53:00Z |
| publishDate | 2018 |
| publisher | Springer Verlag |
| record_format | dspace |
| spelling | um.eprints-210412019-04-19T03:25:27Z http://eprints.um.edu.my/21041/ Neuritogenic and in vitro antioxidant activities of Malaysian Gracilaria manilaensis Yamamoto & Trono Pang, Jun Rui Goh, Veronica Ming Jun Tan, Cheng Yau Phang, Siew Moi Wong, Kah Hui Yow, Yoon Yen Q Science (General) QH Natural history R Medicine Numerous marine-based compounds have been identified as having diverse biological activities. Gracilaria manilaensis Yamamoto & Trono is a red agarophyte endemic to the Malaysia coastal area. It is used as a gelling and thickening agent for food industries. In this study, the rat pheochromocytoma cell line (PC-12Adh) was employed as in vitro model to investigate the neuritogenic activities in hot aqueous, methanol, and ethanol extracts of G. manilaensis. Phytochemical contents and in vitro antioxidant activities of the extracts were also determined. All G. manilaensis extracts were found to increase the percentage of neurite-bearing cells and upregulated the neuronal cytoskeleton of PC-12Adh cells with no inhibition across the tested concentration range (0–0.15 mg mL −1 ). The methanol and ethanol extracts had higher phenolic and flavonoid contents than the hot aqueous extract. They also showed higher antioxidant activities against 2,2-diphenyl-1-pircryhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) free radicals and higher reducing power compared to the hot aqueous extract. It is believed that G. manilaensis may possess potent bioactive compounds that mimic the neuroactivity of the nerve growth factor (NGF) for neuronal survival, development, and differentiation. It has been suggested that agar producer G. manilaensis might be a promising dietary supplement for cognitive health in the prevention of neurological disorders. Springer Verlag 2018 Article PeerReviewed Pang, Jun Rui and Goh, Veronica Ming Jun and Tan, Cheng Yau and Phang, Siew Moi and Wong, Kah Hui and Yow, Yoon Yen (2018) Neuritogenic and in vitro antioxidant activities of Malaysian Gracilaria manilaensis Yamamoto & Trono. Journal of Applied Phycology, 30 (6). pp. 3253-3260. ISSN 0921-8971, DOI https://doi.org/10.1007/s10811-018-1438-x <https://doi.org/10.1007/s10811-018-1438-x>. https://doi.org/10.1007/s10811-018-1438-x doi:10.1007/s10811-018-1438-x |
| spellingShingle | Q Science (General) QH Natural history R Medicine Pang, Jun Rui Goh, Veronica Ming Jun Tan, Cheng Yau Phang, Siew Moi Wong, Kah Hui Yow, Yoon Yen Neuritogenic and in vitro antioxidant activities of Malaysian Gracilaria manilaensis Yamamoto & Trono |
| title | Neuritogenic and in vitro antioxidant activities of Malaysian Gracilaria manilaensis Yamamoto & Trono |
| title_full | Neuritogenic and in vitro antioxidant activities of Malaysian Gracilaria manilaensis Yamamoto & Trono |
| title_fullStr | Neuritogenic and in vitro antioxidant activities of Malaysian Gracilaria manilaensis Yamamoto & Trono |
| title_full_unstemmed | Neuritogenic and in vitro antioxidant activities of Malaysian Gracilaria manilaensis Yamamoto & Trono |
| title_short | Neuritogenic and in vitro antioxidant activities of Malaysian Gracilaria manilaensis Yamamoto & Trono |
| title_sort | neuritogenic and in vitro antioxidant activities of malaysian gracilaria manilaensis yamamoto trono |
| topic | Q Science (General) QH Natural history R Medicine |
| work_keys_str_mv | AT pangjunrui neuritogenicandinvitroantioxidantactivitiesofmalaysiangracilariamanilaensisyamamototrono AT gohveronicamingjun neuritogenicandinvitroantioxidantactivitiesofmalaysiangracilariamanilaensisyamamototrono AT tanchengyau neuritogenicandinvitroantioxidantactivitiesofmalaysiangracilariamanilaensisyamamototrono AT phangsiewmoi neuritogenicandinvitroantioxidantactivitiesofmalaysiangracilariamanilaensisyamamototrono AT wongkahhui neuritogenicandinvitroantioxidantactivitiesofmalaysiangracilariamanilaensisyamamototrono AT yowyoonyen neuritogenicandinvitroantioxidantactivitiesofmalaysiangracilariamanilaensisyamamototrono |