Identification and characterization of neutralization epitopes at VP2 and VP1 of enterovirus A71
Enterovirus A71 (EV-A71) neutralization escape mutants were generated with monoclonal antibody MAB979 (Millipore). The VP2-T141I and VP1-D14N substitutions were identified. Using reverse genetics, infectious clones with these substitutions were constructed and tested by neutralization assay with imm...
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| Format: | Article |
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Wiley
2018
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| author | Nik Nadia, Nik Mohd Nasir Tan, Chee Wah Ong, Kien Chai Sam, I-Ching Chan, Yoke Fun |
| author_facet | Nik Nadia, Nik Mohd Nasir Tan, Chee Wah Ong, Kien Chai Sam, I-Ching Chan, Yoke Fun |
| author_sort | Nik Nadia, Nik Mohd Nasir |
| collection | UM |
| description | Enterovirus A71 (EV-A71) neutralization escape mutants were generated with monoclonal antibody MAB979 (Millipore). The VP2-T141I and VP1-D14N substitutions were identified. Using reverse genetics, infectious clones with these substitutions were constructed and tested by neutralization assay with immune sera from mice and humans. The N-terminus VP1-14 is more important than EF loop VP2-141 in acute human infection, mainly because it recognised IgM present in acute infection. The N-terminus VP1 could be a useful target for diagnostics and therapeutic antibodies in acute infection. |
| first_indexed | 2024-03-06T05:53:14Z |
| format | Article |
| id | um.eprints-21125 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:53:14Z |
| publishDate | 2018 |
| publisher | Wiley |
| record_format | dspace |
| spelling | um.eprints-211252019-05-07T02:20:06Z http://eprints.um.edu.my/21125/ Identification and characterization of neutralization epitopes at VP2 and VP1 of enterovirus A71 Nik Nadia, Nik Mohd Nasir Tan, Chee Wah Ong, Kien Chai Sam, I-Ching Chan, Yoke Fun R Medicine Enterovirus A71 (EV-A71) neutralization escape mutants were generated with monoclonal antibody MAB979 (Millipore). The VP2-T141I and VP1-D14N substitutions were identified. Using reverse genetics, infectious clones with these substitutions were constructed and tested by neutralization assay with immune sera from mice and humans. The N-terminus VP1-14 is more important than EF loop VP2-141 in acute human infection, mainly because it recognised IgM present in acute infection. The N-terminus VP1 could be a useful target for diagnostics and therapeutic antibodies in acute infection. Wiley 2018 Article PeerReviewed Nik Nadia, Nik Mohd Nasir and Tan, Chee Wah and Ong, Kien Chai and Sam, I-Ching and Chan, Yoke Fun (2018) Identification and characterization of neutralization epitopes at VP2 and VP1 of enterovirus A71. Journal of Medical Virology, 90 (6). pp. 1164-1167. ISSN 0146-6615, DOI https://doi.org/10.1002/jmv.25061 <https://doi.org/10.1002/jmv.25061>. https://doi.org/10.1002/jmv.25061 doi:10.1002/jmv.25061 |
| spellingShingle | R Medicine Nik Nadia, Nik Mohd Nasir Tan, Chee Wah Ong, Kien Chai Sam, I-Ching Chan, Yoke Fun Identification and characterization of neutralization epitopes at VP2 and VP1 of enterovirus A71 |
| title | Identification and characterization of neutralization epitopes at VP2 and VP1 of enterovirus A71 |
| title_full | Identification and characterization of neutralization epitopes at VP2 and VP1 of enterovirus A71 |
| title_fullStr | Identification and characterization of neutralization epitopes at VP2 and VP1 of enterovirus A71 |
| title_full_unstemmed | Identification and characterization of neutralization epitopes at VP2 and VP1 of enterovirus A71 |
| title_short | Identification and characterization of neutralization epitopes at VP2 and VP1 of enterovirus A71 |
| title_sort | identification and characterization of neutralization epitopes at vp2 and vp1 of enterovirus a71 |
| topic | R Medicine |
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