Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Acanthamoeba and Cytotoxicity Activities
Poly (DL-lactide-co-glycolide) (PLGA) microspheres were synthesized as delivery system for the natural anti-parasitic compounds, Periglaucine A (PGA) and Betulinic acid (BA). Periglaucine A and Betulinic acid were encapsulated in PLGA nanoparticles by single emulsion method with an average particle...
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| Format: | Article |
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MDPI
2018
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| _version_ | 1796961425338400768 |
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| author | Mahboob, Tooba Nawaz, Muhammad Tan, Tian Chye Samudi, Chandramathi Wiart, Christophe Nissapatorn, Veeranoot |
| author_facet | Mahboob, Tooba Nawaz, Muhammad Tan, Tian Chye Samudi, Chandramathi Wiart, Christophe Nissapatorn, Veeranoot |
| author_sort | Mahboob, Tooba |
| collection | UM |
| description | Poly (DL-lactide-co-glycolide) (PLGA) microspheres were synthesized as delivery system for the natural anti-parasitic compounds, Periglaucine A (PGA) and Betulinic acid (BA). Periglaucine A and Betulinic acid were encapsulated in PLGA nanoparticles by single emulsion method with an average particle size of approximately 100–500 nm. Periglaucine A and Betulinic acid encapsulation efficiency was observed to be 90% and 35% respectively. Anti-Acanthamoeba property of Periglaucine A and Betulinic acid remained intact after encapsulation. PGA-PLGA and BA-PLGA nanoparticles demonstrated inhibition in viability of Acanthamoeba triangularis trophozoites by 74.9%, 59.9%, 49.9% and 71.2%, 52.2%, 88% respectively at concentration of 100 µg/mL, 50 µg/mL and 25 µg/mL. Cytotoxicity of PGA-PLGA and BA-PLGA nanoparticles has been evaluated against lung epithelial cell line and showed dose dependent cytotoxicity value of IC50 2 µg/mL and 20 µg/mL respectively. Futher, increased viability was observed in lung epithelial cell line in higher doses of synthesized polymeric nanoparticles. Results indicate that poly (DL-lactide-co-glycolide) (PLGA) nanoparticles could be exploratory delivery systems for natural products to improve their therapeutic efficacy. |
| first_indexed | 2024-03-06T05:53:58Z |
| format | Article |
| id | um.eprints-21371 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:53:58Z |
| publishDate | 2018 |
| publisher | MDPI |
| record_format | dspace |
| spelling | um.eprints-213712019-05-28T05:31:10Z http://eprints.um.edu.my/21371/ Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Acanthamoeba and Cytotoxicity Activities Mahboob, Tooba Nawaz, Muhammad Tan, Tian Chye Samudi, Chandramathi Wiart, Christophe Nissapatorn, Veeranoot R Medicine Poly (DL-lactide-co-glycolide) (PLGA) microspheres were synthesized as delivery system for the natural anti-parasitic compounds, Periglaucine A (PGA) and Betulinic acid (BA). Periglaucine A and Betulinic acid were encapsulated in PLGA nanoparticles by single emulsion method with an average particle size of approximately 100–500 nm. Periglaucine A and Betulinic acid encapsulation efficiency was observed to be 90% and 35% respectively. Anti-Acanthamoeba property of Periglaucine A and Betulinic acid remained intact after encapsulation. PGA-PLGA and BA-PLGA nanoparticles demonstrated inhibition in viability of Acanthamoeba triangularis trophozoites by 74.9%, 59.9%, 49.9% and 71.2%, 52.2%, 88% respectively at concentration of 100 µg/mL, 50 µg/mL and 25 µg/mL. Cytotoxicity of PGA-PLGA and BA-PLGA nanoparticles has been evaluated against lung epithelial cell line and showed dose dependent cytotoxicity value of IC50 2 µg/mL and 20 µg/mL respectively. Futher, increased viability was observed in lung epithelial cell line in higher doses of synthesized polymeric nanoparticles. Results indicate that poly (DL-lactide-co-glycolide) (PLGA) nanoparticles could be exploratory delivery systems for natural products to improve their therapeutic efficacy. MDPI 2018 Article PeerReviewed Mahboob, Tooba and Nawaz, Muhammad and Tan, Tian Chye and Samudi, Chandramathi and Wiart, Christophe and Nissapatorn, Veeranoot (2018) Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Acanthamoeba and Cytotoxicity Activities. Pathogens, 7 (3). p. 62. ISSN 2076-0817, DOI https://doi.org/10.3390/pathogens7030062 <https://doi.org/10.3390/pathogens7030062>. https://doi.org/10.3390/pathogens7030062 doi:10.3390/pathogens7030062 |
| spellingShingle | R Medicine Mahboob, Tooba Nawaz, Muhammad Tan, Tian Chye Samudi, Chandramathi Wiart, Christophe Nissapatorn, Veeranoot Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Acanthamoeba and Cytotoxicity Activities |
| title | Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Acanthamoeba and Cytotoxicity Activities |
| title_full | Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Acanthamoeba and Cytotoxicity Activities |
| title_fullStr | Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Acanthamoeba and Cytotoxicity Activities |
| title_full_unstemmed | Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Acanthamoeba and Cytotoxicity Activities |
| title_short | Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Acanthamoeba and Cytotoxicity Activities |
| title_sort | preparation of poly dl lactide co glycolide nanoparticles encapsulated with periglaucine a and betulinic acid for in vitro anti acanthamoeba and cytotoxicity activities |
| topic | R Medicine |
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