Extractive Bioconversion of Gamma-Cyclodextrin and Recycling of Cyclodextrin Glycosyltransferase in Liquid Biphasic System Using Thermo-Separating Polymer
An extractive bioconversion conducted on soluble starch with cyclodextrin glycosyltransferase (CGTase) enzyme in ethylene oxide-propylene oxide (EOPO)/potassium phosphates liquid biphasic system (LBS) to extract gamma-cyclodextrin (γ-CD) was examined. A range of EOPO (with potassium phosphates) mole...
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Frontiers Media
2018
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| _version_ | 1825721685755559936 |
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| author | Lin, Yu Kiat Show, Pau Loke Yap, Yee Jiun Ariff, Arbakariya Annuar, Mohamad Suffian Mohamad Lai, Oi Ming Ling, Tau Chuan Ng, Eng Poh |
| author_facet | Lin, Yu Kiat Show, Pau Loke Yap, Yee Jiun Ariff, Arbakariya Annuar, Mohamad Suffian Mohamad Lai, Oi Ming Ling, Tau Chuan Ng, Eng Poh |
| author_sort | Lin, Yu Kiat |
| collection | UM |
| description | An extractive bioconversion conducted on soluble starch with cyclodextrin glycosyltransferase (CGTase) enzyme in ethylene oxide-propylene oxide (EOPO)/potassium phosphates liquid biphasic system (LBS) to extract gamma-cyclodextrin (γ-CD) was examined. A range of EOPO (with potassium phosphates) molecular weights was screen to investigate the effect of the latter on the partioning efficency of CGTase and γ-CD. The results show that the optimal top phase γ-CD yield (74.4%) was reached in 35.0% (w/w) EOPO 970 and 10.0% (w/w) potassium phosphate with 2.0% (w/w) sodium chloride. A theoretical explanation for the effect of NaCl on γ-CD was also presented. After a 2 h bioconversion process, a total of 0.87 mg/mL concentration of γ-CD was produced in the EOPO/ phosphates LBS top phase. After the extraction of top phase from LBS, four continuous repetitive batches were successfully conducted with relative CGTase activity of 1.00, 0.86, 0.45, and 0.40 respectively. |
| first_indexed | 2024-03-06T05:54:07Z |
| format | Article |
| id | um.eprints-21423 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:54:07Z |
| publishDate | 2018 |
| publisher | Frontiers Media |
| record_format | dspace |
| spelling | um.eprints-214232019-05-30T05:05:42Z http://eprints.um.edu.my/21423/ Extractive Bioconversion of Gamma-Cyclodextrin and Recycling of Cyclodextrin Glycosyltransferase in Liquid Biphasic System Using Thermo-Separating Polymer Lin, Yu Kiat Show, Pau Loke Yap, Yee Jiun Ariff, Arbakariya Annuar, Mohamad Suffian Mohamad Lai, Oi Ming Ling, Tau Chuan Ng, Eng Poh Q Science (General) QH Natural history TA Engineering (General). Civil engineering (General) An extractive bioconversion conducted on soluble starch with cyclodextrin glycosyltransferase (CGTase) enzyme in ethylene oxide-propylene oxide (EOPO)/potassium phosphates liquid biphasic system (LBS) to extract gamma-cyclodextrin (γ-CD) was examined. A range of EOPO (with potassium phosphates) molecular weights was screen to investigate the effect of the latter on the partioning efficency of CGTase and γ-CD. The results show that the optimal top phase γ-CD yield (74.4%) was reached in 35.0% (w/w) EOPO 970 and 10.0% (w/w) potassium phosphate with 2.0% (w/w) sodium chloride. A theoretical explanation for the effect of NaCl on γ-CD was also presented. After a 2 h bioconversion process, a total of 0.87 mg/mL concentration of γ-CD was produced in the EOPO/ phosphates LBS top phase. After the extraction of top phase from LBS, four continuous repetitive batches were successfully conducted with relative CGTase activity of 1.00, 0.86, 0.45, and 0.40 respectively. Frontiers Media 2018 Article PeerReviewed Lin, Yu Kiat and Show, Pau Loke and Yap, Yee Jiun and Ariff, Arbakariya and Annuar, Mohamad Suffian Mohamad and Lai, Oi Ming and Ling, Tau Chuan and Ng, Eng Poh (2018) Extractive Bioconversion of Gamma-Cyclodextrin and Recycling of Cyclodextrin Glycosyltransferase in Liquid Biphasic System Using Thermo-Separating Polymer. Frontiers in Chemistry, 6. p. 448. ISSN 2296-2646, DOI https://doi.org/10.3389/fchem.2018.00448 <https://doi.org/10.3389/fchem.2018.00448>. https://doi.org/10.3389/fchem.2018.00448 doi:10.3389/fchem.2018.00448 |
| spellingShingle | Q Science (General) QH Natural history TA Engineering (General). Civil engineering (General) Lin, Yu Kiat Show, Pau Loke Yap, Yee Jiun Ariff, Arbakariya Annuar, Mohamad Suffian Mohamad Lai, Oi Ming Ling, Tau Chuan Ng, Eng Poh Extractive Bioconversion of Gamma-Cyclodextrin and Recycling of Cyclodextrin Glycosyltransferase in Liquid Biphasic System Using Thermo-Separating Polymer |
| title | Extractive Bioconversion of Gamma-Cyclodextrin and Recycling of Cyclodextrin Glycosyltransferase in Liquid Biphasic System Using Thermo-Separating Polymer |
| title_full | Extractive Bioconversion of Gamma-Cyclodextrin and Recycling of Cyclodextrin Glycosyltransferase in Liquid Biphasic System Using Thermo-Separating Polymer |
| title_fullStr | Extractive Bioconversion of Gamma-Cyclodextrin and Recycling of Cyclodextrin Glycosyltransferase in Liquid Biphasic System Using Thermo-Separating Polymer |
| title_full_unstemmed | Extractive Bioconversion of Gamma-Cyclodextrin and Recycling of Cyclodextrin Glycosyltransferase in Liquid Biphasic System Using Thermo-Separating Polymer |
| title_short | Extractive Bioconversion of Gamma-Cyclodextrin and Recycling of Cyclodextrin Glycosyltransferase in Liquid Biphasic System Using Thermo-Separating Polymer |
| title_sort | extractive bioconversion of gamma cyclodextrin and recycling of cyclodextrin glycosyltransferase in liquid biphasic system using thermo separating polymer |
| topic | Q Science (General) QH Natural history TA Engineering (General). Civil engineering (General) |
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