Facile formation of colloidal silver nanoparticles using electrolysis technique and their antimicrobial activity

In this research work, colloidal silver nanoparticles were successfully produced via an electrolysis method and their antibacterial activity was evaluated. This study aims to determine the optimum concentration of colloidal silver nanoparticles to produce an effective inhibition zone for Escherichia...

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Main Author: Lai, Chin Wei
Format: Article
Published: Institution of Engineering and Technology 2018
Subjects:
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author Lai, Chin Wei
author_facet Lai, Chin Wei
author_sort Lai, Chin Wei
collection UM
description In this research work, colloidal silver nanoparticles were successfully produced via an electrolysis method and their antibacterial activity was evaluated. This study aims to determine the optimum concentration of colloidal silver nanoparticles to produce an effective inhibition zone for Escherichia coli bacteria growth. Based on the results obtained, the applied current and reaction times during the electrolysis played a crucial role in determining the colloidal silver nanoparticles’ concentration (ppm). It was found that a minimum content of colloidal silver nanoparticles (0.02 ppm) was required to perform the 0.1 mm inhibition zone diameter on agar medium spread with E. coli bacteria. Interestingly, high concentrations of colloidal silver nanoparticles could exhibit high conductivity (µS/cm) as well as the high intensity of the maximum plasmon peak from the UV/vis absorption spectra at about 390 nm. In conclusion, this research work revealed the destruction of the cytoplasmic membrane and the rupture of the internal organisation of E. -coli bacteria, leading to the leakage of the cytoplasmic contents and cell death after having contact with sufficient concentration of colloidal silver nanoparticles.
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spelling um.eprints-215642019-06-27T03:54:02Z http://eprints.um.edu.my/21564/ Facile formation of colloidal silver nanoparticles using electrolysis technique and their antimicrobial activity Lai, Chin Wei QC Physics QD Chemistry TP Chemical technology In this research work, colloidal silver nanoparticles were successfully produced via an electrolysis method and their antibacterial activity was evaluated. This study aims to determine the optimum concentration of colloidal silver nanoparticles to produce an effective inhibition zone for Escherichia coli bacteria growth. Based on the results obtained, the applied current and reaction times during the electrolysis played a crucial role in determining the colloidal silver nanoparticles’ concentration (ppm). It was found that a minimum content of colloidal silver nanoparticles (0.02 ppm) was required to perform the 0.1 mm inhibition zone diameter on agar medium spread with E. coli bacteria. Interestingly, high concentrations of colloidal silver nanoparticles could exhibit high conductivity (µS/cm) as well as the high intensity of the maximum plasmon peak from the UV/vis absorption spectra at about 390 nm. In conclusion, this research work revealed the destruction of the cytoplasmic membrane and the rupture of the internal organisation of E. -coli bacteria, leading to the leakage of the cytoplasmic contents and cell death after having contact with sufficient concentration of colloidal silver nanoparticles. Institution of Engineering and Technology 2018 Article PeerReviewed Lai, Chin Wei (2018) Facile formation of colloidal silver nanoparticles using electrolysis technique and their antimicrobial activity. Micro & Nano Letters, 13 (3). pp. 407-410. ISSN 1750-0443, DOI https://doi.org/10.1049/mnl.2017.0805 <https://doi.org/10.1049/mnl.2017.0805>. https://doi.org/10.1049/mnl.2017.0805 doi:10.1049/mnl.2017.0805
spellingShingle QC Physics
QD Chemistry
TP Chemical technology
Lai, Chin Wei
Facile formation of colloidal silver nanoparticles using electrolysis technique and their antimicrobial activity
title Facile formation of colloidal silver nanoparticles using electrolysis technique and their antimicrobial activity
title_full Facile formation of colloidal silver nanoparticles using electrolysis technique and their antimicrobial activity
title_fullStr Facile formation of colloidal silver nanoparticles using electrolysis technique and their antimicrobial activity
title_full_unstemmed Facile formation of colloidal silver nanoparticles using electrolysis technique and their antimicrobial activity
title_short Facile formation of colloidal silver nanoparticles using electrolysis technique and their antimicrobial activity
title_sort facile formation of colloidal silver nanoparticles using electrolysis technique and their antimicrobial activity
topic QC Physics
QD Chemistry
TP Chemical technology
work_keys_str_mv AT laichinwei facileformationofcolloidalsilvernanoparticlesusingelectrolysistechniqueandtheirantimicrobialactivity