Gene expression profile of marine Chlorella strains from different latitudes: stress and recovery under elevated temperatures
Global warming, as a consequence of climate change, poses a critical threat to marine life, including algae. Studies on algal response at the molecular level to temperature stress have been significantly improved by advances in omics technologies. Algae are known to employ various strategies in resp...
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| Format: | Article |
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Springer Verlag
2018
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| author | Barati, Bahram Lim, Phaik Eem Gan, Sook Yee Poong, Sze Wan Phang, Siew Moi |
| author_facet | Barati, Bahram Lim, Phaik Eem Gan, Sook Yee Poong, Sze Wan Phang, Siew Moi |
| author_sort | Barati, Bahram |
| collection | UM |
| description | Global warming, as a consequence of climate change, poses a critical threat to marine life, including algae. Studies on algal response at the molecular level to temperature stress have been significantly improved by advances in omics technologies. Algae are known to employ various strategies in response to heat stress. For example, algae regulate starch synthesis to provide energy for the cell or rebuild the damaged subunits of photosystems to regain photosynthetic activity. The aim of the present study is to examine the expression of selected photosynthesis-related genes of marine Chlorella originating from different latitudes, in response to heat stress and during the recovery period. In this study, marine Chlorella strains from the Antarctic, temperate region, and the tropics were grown at their ambient and stress-inducing temperatures. The maximum quantum efficiency (F v /F m ) photosynthetic parameter was used to assess their stress levels. When subjected to heat stress, the F v /F m began to decline and when it reached ~ 0.2, the cultures were transferred to their respective ambient temperature for recovery. Total RNA was isolated from these cultures at F v /F m ~ 0.4, 0.2, and when it regained 0.4 during recovery. The expression of four genes including psbA, psaB, psbC, and rbcL was analyzed using RT-PCR. The housekeeping gene, histone subunit three (H3) was used for data normalization. Studying the genes involved in the adaptation mechanisms would enhance our knowledge on algal adaptation pathways and pave the way for genetic engineers to develop more tolerant strains. |
| first_indexed | 2024-03-06T05:56:43Z |
| format | Article |
| id | um.eprints-22355 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:56:43Z |
| publishDate | 2018 |
| publisher | Springer Verlag |
| record_format | dspace |
| spelling | um.eprints-223552019-09-13T07:40:15Z http://eprints.um.edu.my/22355/ Gene expression profile of marine Chlorella strains from different latitudes: stress and recovery under elevated temperatures Barati, Bahram Lim, Phaik Eem Gan, Sook Yee Poong, Sze Wan Phang, Siew Moi Q Science (General) QH Natural history Global warming, as a consequence of climate change, poses a critical threat to marine life, including algae. Studies on algal response at the molecular level to temperature stress have been significantly improved by advances in omics technologies. Algae are known to employ various strategies in response to heat stress. For example, algae regulate starch synthesis to provide energy for the cell or rebuild the damaged subunits of photosystems to regain photosynthetic activity. The aim of the present study is to examine the expression of selected photosynthesis-related genes of marine Chlorella originating from different latitudes, in response to heat stress and during the recovery period. In this study, marine Chlorella strains from the Antarctic, temperate region, and the tropics were grown at their ambient and stress-inducing temperatures. The maximum quantum efficiency (F v /F m ) photosynthetic parameter was used to assess their stress levels. When subjected to heat stress, the F v /F m began to decline and when it reached ~ 0.2, the cultures were transferred to their respective ambient temperature for recovery. Total RNA was isolated from these cultures at F v /F m ~ 0.4, 0.2, and when it regained 0.4 during recovery. The expression of four genes including psbA, psaB, psbC, and rbcL was analyzed using RT-PCR. The housekeeping gene, histone subunit three (H3) was used for data normalization. Studying the genes involved in the adaptation mechanisms would enhance our knowledge on algal adaptation pathways and pave the way for genetic engineers to develop more tolerant strains. Springer Verlag 2018 Article PeerReviewed Barati, Bahram and Lim, Phaik Eem and Gan, Sook Yee and Poong, Sze Wan and Phang, Siew Moi (2018) Gene expression profile of marine Chlorella strains from different latitudes: stress and recovery under elevated temperatures. Journal of Applied Phycology, 30 (6). pp. 3121-3130. ISSN 0921-8971, DOI https://doi.org/10.1007/s10811-018-1588-x <https://doi.org/10.1007/s10811-018-1588-x>. https://doi.org/10.1007/s10811-018-1588-x doi:10.1007/s10811-018-1588-x |
| spellingShingle | Q Science (General) QH Natural history Barati, Bahram Lim, Phaik Eem Gan, Sook Yee Poong, Sze Wan Phang, Siew Moi Gene expression profile of marine Chlorella strains from different latitudes: stress and recovery under elevated temperatures |
| title | Gene expression profile of marine Chlorella strains from different latitudes: stress and recovery under elevated temperatures |
| title_full | Gene expression profile of marine Chlorella strains from different latitudes: stress and recovery under elevated temperatures |
| title_fullStr | Gene expression profile of marine Chlorella strains from different latitudes: stress and recovery under elevated temperatures |
| title_full_unstemmed | Gene expression profile of marine Chlorella strains from different latitudes: stress and recovery under elevated temperatures |
| title_short | Gene expression profile of marine Chlorella strains from different latitudes: stress and recovery under elevated temperatures |
| title_sort | gene expression profile of marine chlorella strains from different latitudes stress and recovery under elevated temperatures |
| topic | Q Science (General) QH Natural history |
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