Experimental and computational approaches to reveal the potential of Ficus deltoidea leaves extract as α-amylase inhibitor
Ficus deltoidea leaves extract are known to have good therapeutic properties such as antioxidant, anti-inflammatory and anti-diabetic. We showed that 50% ethanol-water extract of F. deltoidea leaves and its pungent compounds vitexin and isovitexin exhibited significant (p < 0.05) α-amylase inhibi...
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Taylor & Francis
2018
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| author | Abu Bakar, Amirul Ridzuan Manaharan, Thamilvaani Merican, Amir Feisal Mohamad, Saharuddin |
| author_facet | Abu Bakar, Amirul Ridzuan Manaharan, Thamilvaani Merican, Amir Feisal Mohamad, Saharuddin |
| author_sort | Abu Bakar, Amirul Ridzuan |
| collection | UM |
| description | Ficus deltoidea leaves extract are known to have good therapeutic properties such as antioxidant, anti-inflammatory and anti-diabetic. We showed that 50% ethanol-water extract of F. deltoidea leaves and its pungent compounds vitexin and isovitexin exhibited significant (p < 0.05) α-amylase inhibition with IC50 (vitexin: 4.6 μM [0.02 μg/mL]; isovitexin: 0.06 μg/mL [13.8 μM] and DPPH scavenging with IC50 (vitexin: 92.5 μM [0.4 μg/mL]; isovitexin: 0.5 μg/mL [115.4 μM]). Additionally, molecular docking analysis confirmed that vitexin has a higher binding affinity (-7.54 kcal/mol) towards α-amylase compared to isovitexin (−5.61 kcal/mol). On the other hand, the molecular dynamics findings showed that vitexin-α-amylase complex is more stable during the simulation of 20 ns when compared to the isovitexin-α-amylase complex. Our results suggest that vitexin is more potent and stable against α-amylase enzyme, thus it could develop as a therapeutic drug for the treatment of diabetes. |
| first_indexed | 2024-03-06T05:57:48Z |
| format | Article |
| id | um.eprints-22720 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:57:48Z |
| publishDate | 2018 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | um.eprints-227202019-10-08T07:59:51Z http://eprints.um.edu.my/22720/ Experimental and computational approaches to reveal the potential of Ficus deltoidea leaves extract as α-amylase inhibitor Abu Bakar, Amirul Ridzuan Manaharan, Thamilvaani Merican, Amir Feisal Mohamad, Saharuddin Q Science (General) QH Natural history Ficus deltoidea leaves extract are known to have good therapeutic properties such as antioxidant, anti-inflammatory and anti-diabetic. We showed that 50% ethanol-water extract of F. deltoidea leaves and its pungent compounds vitexin and isovitexin exhibited significant (p < 0.05) α-amylase inhibition with IC50 (vitexin: 4.6 μM [0.02 μg/mL]; isovitexin: 0.06 μg/mL [13.8 μM] and DPPH scavenging with IC50 (vitexin: 92.5 μM [0.4 μg/mL]; isovitexin: 0.5 μg/mL [115.4 μM]). Additionally, molecular docking analysis confirmed that vitexin has a higher binding affinity (-7.54 kcal/mol) towards α-amylase compared to isovitexin (−5.61 kcal/mol). On the other hand, the molecular dynamics findings showed that vitexin-α-amylase complex is more stable during the simulation of 20 ns when compared to the isovitexin-α-amylase complex. Our results suggest that vitexin is more potent and stable against α-amylase enzyme, thus it could develop as a therapeutic drug for the treatment of diabetes. Taylor & Francis 2018 Article PeerReviewed Abu Bakar, Amirul Ridzuan and Manaharan, Thamilvaani and Merican, Amir Feisal and Mohamad, Saharuddin (2018) Experimental and computational approaches to reveal the potential of Ficus deltoidea leaves extract as α-amylase inhibitor. Natural Product Research, 32 (4). pp. 473-476. ISSN 1478-6419, DOI https://doi.org/10.1080/14786419.2017.1312393 <https://doi.org/10.1080/14786419.2017.1312393>. https://doi.org/10.1080/14786419.2017.1312393 doi:10.1080/14786419.2017.1312393 |
| spellingShingle | Q Science (General) QH Natural history Abu Bakar, Amirul Ridzuan Manaharan, Thamilvaani Merican, Amir Feisal Mohamad, Saharuddin Experimental and computational approaches to reveal the potential of Ficus deltoidea leaves extract as α-amylase inhibitor |
| title | Experimental and computational approaches to reveal the potential of Ficus deltoidea leaves extract as α-amylase inhibitor |
| title_full | Experimental and computational approaches to reveal the potential of Ficus deltoidea leaves extract as α-amylase inhibitor |
| title_fullStr | Experimental and computational approaches to reveal the potential of Ficus deltoidea leaves extract as α-amylase inhibitor |
| title_full_unstemmed | Experimental and computational approaches to reveal the potential of Ficus deltoidea leaves extract as α-amylase inhibitor |
| title_short | Experimental and computational approaches to reveal the potential of Ficus deltoidea leaves extract as α-amylase inhibitor |
| title_sort | experimental and computational approaches to reveal the potential of ficus deltoidea leaves extract as α amylase inhibitor |
| topic | Q Science (General) QH Natural history |
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