Serum from Nipah virus patients recognises recombinant viral proteins produced in Escherichia coli
The genes for Nipah virus (NiV) proteins were amplified from viral RNA, cloned into the plasmid pTriEx-3 Hygro, expressed, and purified using immobilized metal affinity chromatography. The recombinant N, F, and G NiV proteins (rNiV-N, rNiV-F, and rNiV-G), were successfully expressed in Escherichia c...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Published: |
Institute of Infectious Diseases
2017
|
| Subjects: |