Serum from Nipah virus patients recognises recombinant viral proteins produced in Escherichia coli
The genes for Nipah virus (NiV) proteins were amplified from viral RNA, cloned into the plasmid pTriEx-3 Hygro, expressed, and purified using immobilized metal affinity chromatography. The recombinant N, F, and G NiV proteins (rNiV-N, rNiV-F, and rNiV-G), were successfully expressed in Escherichia c...
Main Authors: | Tiong, Vunjia, Lam, Chui Wan, Phoon, Wai Hong, AbuBakar, Sazaly, Chang, Li Yen |
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Format: | Article |
Published: |
Institute of Infectious Diseases
2017
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Subjects: |
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