Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production
The pellet diameter and morphology of an underutilized European Ganoderma pfeifferi (EGP) mycelium was studied in a repeated-batch fermentation (RBF) for exopolysaccharide (EPS) production. In preliminary fermentation, growth screening was performed for initial pH, glucose concentration, and agitati...
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| Format: | Article |
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Elsevier
2019
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| _version_ | 1796961806423425024 |
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| author | Supramani, Sugenendran Jailani, Nurulhuda Ramarao, Kivaandra Mohd Zain, Nurul Amalina Klaus, Anita Ahmad, Rahayu Wan-Mohtar, Wan Abd Al Qadr Imad |
| author_facet | Supramani, Sugenendran Jailani, Nurulhuda Ramarao, Kivaandra Mohd Zain, Nurul Amalina Klaus, Anita Ahmad, Rahayu Wan-Mohtar, Wan Abd Al Qadr Imad |
| author_sort | Supramani, Sugenendran |
| collection | UM |
| description | The pellet diameter and morphology of an underutilized European Ganoderma pfeifferi (EGP) mycelium was studied in a repeated-batch fermentation (RBF) for exopolysaccharide (EPS) production. In preliminary fermentation, growth screening was performed for initial pH, glucose concentration, and agitation speed. At 30 °C, the favourable conditions for EPS production (0.58 g/L) and EPS productivity (0.058 g/L day −1 ) were 15 g/L glucose, initial pH 6, and 100 rpm, while the highest dry cell weight (DCW; 3.63 g/L) was achieved at 9 g/L glucose, initial pH 4, and 120 rpm. Morphologically verified, large, compact pellets (diameter: 40 μm < d < 40.67 μm) were associated with high DCW while small dispersed pellets (diameter: 11.67 μm < d < 14.67 μm) were associated with high EPS production. In RBF, EGP mycelium exhibited the ability to self-immobilize and high stability for repeated use with increasing smooth pellet diameter from RBF1 (7.33 μm < d < 7.67 μm) to RBF4 (16.67 μm < d < 18.33 μm). The fermentation period was subsequently shortened from 48 (batch) to 24 days in four consecutive cycles of RBF, and the productivity of EPS increased from 0.053 g/L day −1 to 0.108 g/L day −1 . In an FTIR analysis, crude EPS of EGP showed the presence of β-glucan characteristics at 1075 cm −1 and 891 cm −1 wavelengths, similar with European G. lucidum BCCM 31549. In addition, 1 H NMR showed similar β-glycosidic linkages when compared with laminarin standard. The proposed strategy demonstrated that G. pfeifferi pellet morphology can withstand extended fermentation cycles for efficient EPS production. © 2019 Elsevier Ltd |
| first_indexed | 2024-03-06T05:59:38Z |
| format | Article |
| id | um.eprints-23360 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:59:38Z |
| publishDate | 2019 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | um.eprints-233602020-01-09T01:04:06Z http://eprints.um.edu.my/23360/ Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production Supramani, Sugenendran Jailani, Nurulhuda Ramarao, Kivaandra Mohd Zain, Nurul Amalina Klaus, Anita Ahmad, Rahayu Wan-Mohtar, Wan Abd Al Qadr Imad Q Science (General) QR Microbiology The pellet diameter and morphology of an underutilized European Ganoderma pfeifferi (EGP) mycelium was studied in a repeated-batch fermentation (RBF) for exopolysaccharide (EPS) production. In preliminary fermentation, growth screening was performed for initial pH, glucose concentration, and agitation speed. At 30 °C, the favourable conditions for EPS production (0.58 g/L) and EPS productivity (0.058 g/L day −1 ) were 15 g/L glucose, initial pH 6, and 100 rpm, while the highest dry cell weight (DCW; 3.63 g/L) was achieved at 9 g/L glucose, initial pH 4, and 120 rpm. Morphologically verified, large, compact pellets (diameter: 40 μm < d < 40.67 μm) were associated with high DCW while small dispersed pellets (diameter: 11.67 μm < d < 14.67 μm) were associated with high EPS production. In RBF, EGP mycelium exhibited the ability to self-immobilize and high stability for repeated use with increasing smooth pellet diameter from RBF1 (7.33 μm < d < 7.67 μm) to RBF4 (16.67 μm < d < 18.33 μm). The fermentation period was subsequently shortened from 48 (batch) to 24 days in four consecutive cycles of RBF, and the productivity of EPS increased from 0.053 g/L day −1 to 0.108 g/L day −1 . In an FTIR analysis, crude EPS of EGP showed the presence of β-glucan characteristics at 1075 cm −1 and 891 cm −1 wavelengths, similar with European G. lucidum BCCM 31549. In addition, 1 H NMR showed similar β-glycosidic linkages when compared with laminarin standard. The proposed strategy demonstrated that G. pfeifferi pellet morphology can withstand extended fermentation cycles for efficient EPS production. © 2019 Elsevier Ltd Elsevier 2019 Article PeerReviewed Supramani, Sugenendran and Jailani, Nurulhuda and Ramarao, Kivaandra and Mohd Zain, Nurul Amalina and Klaus, Anita and Ahmad, Rahayu and Wan-Mohtar, Wan Abd Al Qadr Imad (2019) Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production. Biocatalysis and Agricultural Biotechnology, 19. p. 101118. ISSN 1878-8181, DOI https://doi.org/10.1016/j.bcab.2019.101118 <https://doi.org/10.1016/j.bcab.2019.101118>. https://doi.org/10.1016/j.bcab.2019.101118 doi:10.1016/j.bcab.2019.101118 |
| spellingShingle | Q Science (General) QR Microbiology Supramani, Sugenendran Jailani, Nurulhuda Ramarao, Kivaandra Mohd Zain, Nurul Amalina Klaus, Anita Ahmad, Rahayu Wan-Mohtar, Wan Abd Al Qadr Imad Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production |
| title | Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production |
| title_full | Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production |
| title_fullStr | Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production |
| title_full_unstemmed | Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production |
| title_short | Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production |
| title_sort | pellet diameter and morphology of european ganoderma pfeifferi in a repeated batch fermentation for exopolysaccharide production |
| topic | Q Science (General) QR Microbiology |
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